Application of tetrahedral -deoxyribonucleic acid electrochemistry platform coupling aptazymes and hybridized hairpin reactions for the measurement of extracellular adenosine triphosphate in plants

Zhao, Guoyan; Liu, Yongmei; Du, Jie; Zhang, Huizi; Feng, Hongmei; Lu, Xiaoquan

doi:10.1016/j.aca.2021.338681

Cited by 7 publications

(3 citation statements)

References 57 publications

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“…The aptazyme possesses the potential to be integrated in a label-free biosensing system in which the DNAzyme activity is activated once the aptamer-target binding occurs. This system's merits include quick response, simplicity, high sensing capability and selectivity [43,44]. However, there is no documentation of an aptazyme for the chemiluminescence detection of ZEN.…”

Section: Introductionmentioning

confidence: 99%

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

Guan

Neng

et al. 2023

Biosensors

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Zearalenone (ZEN), one of the most frequently occurring mycotoxin contaminants in foods and feeds, poses considerable threat to human and animal health, owing to its acute and chronic toxicities. Thus, rapid and accurate detection of ZEN has attracted broad research interest. In this work, a novel and label-free chemiluminescence aptasensor based on a ZEN aptamer and a G-quadruplex DNAzyme was constructed. It was established on a competitive assay between ZEN and an auxiliary DNA for the aptamer, leading to activation of the G-quadruplex/hemin DNAzyme and subsequent signal amplification by chemiluminescence generation after substrate addition. To maximize the detection sensitivity, numerous key parameters including truncated aptamers were optimized with molecular docking analysis. Upon optimization, our aptasensor exhibited a perfect linear relationship (R2 = 0.9996) for ZEN detection in a concentration range of 1–100 ng/mL (3.14–314.10 nM) within 40 min, achieving a detection limit of 2.85 ng/mL (8.95 nM), which was a 6.7-fold improvement over that before optimization. Most importantly, the aptasensor obtained a satisfactory recovery rate of 92.84–137.27% and 84.90–124.24% for ZEN-spiked wheat and maize samples, respectively. Overall, our label-free chemiluminescence aptasensor displayed simplicity, sensitivity, specificity and practicality in real samples, indicating high application prospects in the food supply chain for rapid detection of ZEN.

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Section: Introductionmentioning

confidence: 99%

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

Guan

Neng

et al. 2023

Biosensors

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“…The vulnerability of enzymes significantly limited the applications of enzyme amplified-HCR nucleic acid assays in complex biological samples and was also not convenient for transport and storage. Alternatively, both G-quadruplex/hemin and DNAzyme, which were dependent on the catalytic activity of the DNA/RNA itself, somehow overcame the drawbacks of protein-based enzymes and were successfully applied in many studies [29][30][31][32]. In addition to the enzymes, nanoparticles harboring complexes with fluorescent or enzymatic components are reported to be an effective strategy for amplifying the signal and significantly improving the LOD [33,34].…”

Section: Introductionmentioning

confidence: 99%

Detection of Hepatitis C virus RNA using a novel hybridization chain reaction method that competitively dampens cascade amplification

Zhang

Yao

et al. 2023

PLoS ONE

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The hybridization chain reaction (HCR) is widely used for biosensing. However, HCR does not provide the required sensitivity. In this study, we reported a method to improve the sensitivity of HCR by dampening the cascade amplification. First, we designed a biosensor based on HCR, and an initiator DNA was used to trigger the cascade amplification. Optimization of the reaction was then performed, and the results showed that the limit of detection (LOD) for the initiator DNA was about 2.5 nM. Second, we designed a series of inhibitory DNAs to dampen the HCR cascade amplification, and DNA dampeners (50 nM) were applied in the presence of the DNA initiator (50 nM). One of the DNA dampeners (D5) showed the best inhibitory efficiency of greater than 80%. This was further applied at concentrations ranging from 0 nM to 10 nM to prohibit the HCR amplification caused by a 2.5 nM initiator DNA (the limit of detection for this initiator DNA). The results showed that 0.156 nM of D5 could significantly inhibit the signal amplification (p<0.05). Additionally, the limit of detection for the dampener D5 was 16 times lower than that for the initiator DNA. Based on this detection method, we achieved a detection limit as low as 0.625 nM for HCV-RNAs. In summary, we developed a novel method with improved sensitivity to detect the target designed to prohibit the HCR cascade. Overall, this method could be used to qualitatively detect the presence of single-stranded DNA/RNA.

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“…2 Hybridized chain reaction (HCR), catalytic hairpin assembly (CHA), and rolling circle amplification (RCA) have been used to improve the sensitivity of aptazyme-based detection. [14][15][16][17] Nevertheless, nucleic acid amplification techniques suffer from some shortcomings, including the design of complex primers, expensive reagents, sophisticated procedures and specialized equipment. Therefore, there is an urgent need for a simple, rapid, low-cost and universal signal enhancement strategy for the detection of low-abundance targets.…”

Section: Introductionmentioning

confidence: 99%

Aptazyme-induced cascade amplification integrated with a volumetric bar-chart chip for highly sensitive detection of aflatoxin B1 and adenosine triphosphate

Han

Liu

Zhao

et al. 2022

Analyst

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Application of tetrahedral -deoxyribonucleic acid electrochemistry platform coupling aptazymes and hybridized hairpin reactions for the measurement of extracellular adenosine triphosphate in plants

Cited by 7 publications

References 57 publications

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

A Novel and Label-Free Chemiluminescence Detection of Zearalenone Based on a Truncated Aptamer Conjugated with a G-Quadruplex DNAzyme

Detection of Hepatitis C virus RNA using a novel hybridization chain reaction method that competitively dampens cascade amplification

Aptazyme-induced cascade amplification integrated with a volumetric bar-chart chip for highly sensitive detection of aflatoxin B1 and adenosine triphosphate

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