Aptazyme-induced cascade amplification integrated with a volumetric bar-chart chip for highly sensitive detection of aflatoxin B1 and adenosine triphosphate

Han, Yuanyue; Liu, Xinli; Zhao, Qiao; Gao, Yanfeng; Zhou, Dongtao; Long, Wenxiu; Wang, Yuzhen; Song, Yujun

doi:10.1039/d2an00650b

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…Interestingly, a bifunctional aptazyme consisting of Apt and G‐quadruplex was selected as the signal probe for visually quantify AFB 1 and adenosine triphosphate (Han, Liu et al., 2022). Herein, upon the correct recognition of analytes, the aptazymes exhibited a strong DNAzyme activity to catalyze the formation of a polydopamine (PDA) layer on the magnetic silica NPs, which were excellent carriers for PtNPs.…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

“…In virtue of the remarkable specificity of biorecognition elements toward analytes, immunosensor and aptasensors are capable of effectively extracting mycotoxins from complex matrices. Additionally, the integration of immunosensors and aptasensors with smart devices (e.g., smartphone and microchip) has enabled immediate signal readout, pushing a substantial advancement in the field of one‐site and in‐house detection of mycotoxins (Han, Liu et al., 2022; Jafari et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

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Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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“…For example, Han and his colleagues designed a three-channel volumetric bar graph chip (TV-chip) for the visual quantification of aflatoxin B1 and adenosine triphosphate using DNAzyme G-quadruplex, with detection limits of 0.075 pM and 0.818 pM. 19 Similarly, Song and his colleagues designed a type of volumetric bar chart to detect creatine kinase, troponin I, and myoglobin biomarkers, achieving a specificity of 87.5% and a sensitivity of 95.8%. 1 Although using volumetric chips is an innovative approach, reagent reactions may inconsistently produce gas, which can lead to false responses.…”

Section: Introductionmentioning

confidence: 99%

Designing and prototyping a novel biosensor based on a volumetric bar-chart chip for urea detection

Samadi Khezri,

Housaindokht,

Firouzi

2024

Lab Chip

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“…MSNPs-NH 2 was prepared according to the solvothermal method and modified with antibodies using a glutaraldehyde cross-linking method to produce MSNPs-NH 2 /Ab1. 33 The processes are as follows: MSNPs-NH 2 (1 mg) was dispersed in 2 mL of phosphate-buffered saline (PBS, pH 7.4) by sonication, and then 100 μL of glutaraldehyde (25%, v/v) was added and shaken for 1.5 h at room temperature. The mixture was rinsed and washed five times with PBS.…”

Section: ■ Introductionmentioning

confidence: 99%

Metal–Organic Framework-DNA Bio-Barcodes Amplified CRISPR/Cas12a Assay for Ultrasensitive Detection of Protein Biomarkers

et al. 2022

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CRISPR/Cas12a shows excellent potential in disease diagnostics. However, insensitive signal conversion strategies hindered its application in detecting protein biomarkers. Here, we report a metal–organic framework (MOF)-based DNA bio-barcode integrated with the CRISPR/Cas12a system for ultrasensitive detection of protein biomarkers. In this work, zirconium-based MOF nanoparticles were comodified with antibodies and bio-barcode phosphorylated DNA as an efficient signal converter, which not only recognized the protein biomarker to form the sandwich complex but also released the bio-barcode DNA activators after MOF dissociation to activate the trans-cleavage activity of Cas12a. Due to the obvious advantages, including numerous loaded oligonucleotides, a convenient release process, and the nontoxic release reagent, this MOF-DNA bio-barcode strategy could amplify the CRISPR/Cas12a system to achieve simple and highly sensitive detection of tumor protein biomarkers with detection limits of 0.03 pg/mL (PSA) and 0.1 pg/mL (CEA), respectively. Furthermore, this platform could detect PSA directly in clinical serum samples, offering a powerful tool for early disease diagnosis.

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Aptazyme-induced cascade amplification integrated with a volumetric bar-chart chip for highly sensitive detection of aflatoxin B1 and adenosine triphosphate

Abstract: A simple aptazymes-induced cascade signal amplification (denoted as ACSA) was integrated with a triple-channel volumetric bar-chart chip (TV-Chip) to visually quantitate aflatoxin B1 (AFB1) and adenosine triphosphate (ATP). Bifunctional aptazymes...

Cited by 9 publications

References 39 publications

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Designing and prototyping a novel biosensor based on a volumetric bar-chart chip for urea detection

Metal–Organic Framework-DNA Bio-Barcodes Amplified CRISPR/Cas12a Assay for Ultrasensitive Detection of Protein Biomarkers

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