Application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste

Imandi, Sarat Babu; Bandaru, Veera Venkata Ratnam; Somalanka, Subba Rao; Bandaru, Sita Ramalakshmi; Garapati, Hanumantha Rao

doi:10.1016/j.biortech.2007.08.071

Cited by 179 publications

(93 citation statements)

References 17 publications

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“…Ushio et al, (1996) also optimized 1.0ml of inoculum for maximum lipase production [50]. Imandi et al, (2013) reported a 2ml of inoculums [51,52].…”

Section: Effect Of Inoculum Sizementioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

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“…Ushio et al, (1996) also optimized 1.0ml of inoculum for maximum lipase production [50]. Imandi et al, (2013) reported a 2ml of inoculums [51,52].…”

Section: Effect Of Inoculum Sizementioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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“…In recent years, glycerol has been successfully used as a carbon source in the production of citric acid with Y. lipolytica [6,18,19,26,27]. In the literature, it has been reported that in addition to studies in which pure glycerol is used as a substrate, there are other studies in which crude glycerol is used as a byproduct in the production of biodiesel and soap [2,3,19,22,28].…”

Section: Discussionmentioning

confidence: 99%

Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Uçar¹,

Çelik²,

Akpınar³

et al. 2014

Turk J Bioch

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Objective: The goal of this study was to determine the influence of various carbon sources on the citric and isocitric acid production by various Yarrowia lipolytica strains. Methods:The yeasts used in our study were first investigated for organic acid production using screening media. Then, the effect of several complex carbon sources on the citric and isocitric acid production of selected yeast strains was investigated. The amount of citric and isocitric acid production was determined via enzymatic reactions. Results: In this study, 22 Y. lipolytica strains were investigated for the organic acid production. Among these strains, 2 strains (TEM YL 3 and TEM YL 20) were found to be highest organicacid producer. Taken all results together, the highest amounts of citric acid (66.2 g/L for TEM YL 3, 50.0 g/L for TEM YL 20) were observed in the production medium containing sunflower oil. Conclusion: Citric acid consumption, and thus, the need for it are constantly rising in our country, which imports citric acid. Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains. Key Words: Organic acid production, citric acid, isocitric acid, Yarrowia lipolytica. Conflict of Interest: Authors do not have any conflict of interest. ÖZETAmaç: Bu çalışmada, Yarrowia lipolytica suşları tarafından sitrik ve izositrik asit üretimi üzerine çeşitli karbon kaynaklarının etkisinin saptanması amaçlanmıştır. Metod: İlk olarak çalışmamızda kullandığımız mayalar, organik asit üretimleri açısından tarama besiyerlerinde test edilmişlerdir. Daha sonra, bu maya strainleri tarafından üretilen sitrik ve izositrik asit üretimi üzerine farklı karbon kaynaklarının etkisi araştırılmıştır. Sitrik ve izositrik asit üretim miktarları enzimatik test kitleri kullanılarak saptanmıştır. Bulgular: Bu çalışmada, organik asit üretimi açısından 22 Y. lipolytica suşu incelenmiştir. Bu 22 maya suşu arasından iki suşun (TEM YL 3 ve TEM YL 20), en yüksek organik asit üretici maya suşları olduğu bulunmuştur. Bütün sonuçlar değerlendirildiğinde, en yüksek sitrik asit üretimi (TEM YL 3 için 66.2 g/L, TEM YL 20 için 50.0 g/L) ayçiçek yağı içeren üretim ortamında saptanmıştır. Sonuç: Özellikle sitrik asit ithalatı yapılan ülkemizde, sitrik asit tüketimi ve ona olan ihtiyaç sürekli olarak artmaktadır. Atık karbon kaynaklarının değerlendirilmesi ve maliyeti düşük ancak verimi yüksek sitrik asit üreten yeni suşların kullanılarak, maksimum sitrik asit verimini sağlayacak daha ileri çalışmalara ihtiyaç duyulmaktadır. Material and Methods Microorganisms and growth conditionsDomestic Y. lipolytica strains TEM YL 3 and TEM YL 20 had been isolated from cheese [14] and identified with phenotypic and genotypic approach [14,15]. Initially yeast cells were grown aerobically at 27°C in a liquid YPD medium including (1.0 % yeast extract, 2.0 % peptone, and 2.0 % glucose). Screening of total organic acid pro...

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“…The methanol's effect in producing citric acid depends on osmotic pressure. From another aspect, methanol operates as a source of carbon (17,19,20).…”

Section: Discussionmentioning

confidence: 99%

“…The initial and remaining sugar levels were measured by dinitrosalicylic acid (DNS) method while the level of citric acid was measured using acetic anhydride pyridine method (18,19).…”

Section: Determining Methodsmentioning

confidence: 99%

Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

Yadegary

Hamidi

Alavi

et al. 2013

Jundishapur J Microbiol

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Background: Citric acid is produced in insignificant quantities in Iran. Despite its great range of utilizations, and from another aspect, high level of production of sugarcane bagasse, the related problems arising from maintenance of this acid require thinking of a measure in the direction of its optimal usage and production. Objectives: The objective of the present study is to obtain effectual variables in producing citric acid from sugarcane bagasse through Solid State Fermentation (SSF) method using Aspergillus niger mold and to optimize its mass production by employing Taguchi method. Materials and Methods:The effective parameters such as spore inoculation level, methanol percentage, solvent type, spore age, humidity percentage, initial pH of substrate, fermentation period and temperature, initial sugar percentage, autoclaving duration, nitrogen source and etc. were studied for producing citric acid from sugarcane bagasse with respect to Tagouchi method. Results: By considering the findings obtained from the tests, the highest production rate of citric acid g/kg out of untreated sugarcane bagasse is 75.45 based on the consumed sugar and a yield of 15.1 g/kg of sugarcane was achieved per day. Application of sodium hydroxide and acid pretreated sugarcane bagasse increased the production of citric acid in such a fashion that the production rates were 97.81 g/kg and 87.32 g/kg of sugarcane bagasse, respectively, compared to sodium hydroxide and acid untreated sugarcane bagasse. Conclusions:The obtained findings in the present study indicated that sugarcane bagasse is an ideal substrate in producing citric acid and the aforementioned process could be considered as a beneficial and cost-effective method in citric acid production.

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Application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste

Cited by 179 publications

References 17 publications

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

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