Application of silicon in plant tissue culture

Sahebi, Mahbod; Hanafi, Mohamed; Azizi, Parisa

doi:10.1007/s11627-016-9757-6

Cited by 34 publications

(22 citation statements)

References 50 publications

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“…As mentioned earlier, one of the most important effects of silicon on plants is to increase the plants photosynthetic activity and nutrient uptake (Sahebi et al 2016). Although silicon is not being a part of any plant tissue culture media, the positive role of Si to improve physiological and morphologic potentials such as increasing callus growth and regeneration has been shown in different in vitro cultures (Ma 2004;He et al 2013;Sivanesan and Park 2014).…”

Section: Effects Of Various Adjuvant Materials Concentrations On Plantmentioning

confidence: 93%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, L-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L -1 of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg

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Section: Effects Of Various Adjuvant Materials Concentrations On Plantmentioning

confidence: 93%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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“…Moreover, in vitro approaches also offer better prospects during the selection stage in a breeding program since some environmental limitations can be solved using this system (KHALID & AFTAB, 2016). Rezende et al An alternative recently studied that aimed to reduce the saline effects on plants is to use the silicon fertilization (SAHEBI et al, 2016). Silicon (Si) is found in greater quantities on Earth's crust, and its essentiality for plants has not been proven yet.…”

Section: Introductionmentioning

confidence: 99%

Salt stress and exogenous silicon influence physiological and anatomical features of in vitro-grown cape gooseberry

et al. 2017

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Salt stress is one of several major abiotic stresses that affect plant growth and development, and there are many evidences that silicon can ameliorate the injuries caused by high salinity. This study presents the results of an assay concerning: (1) the effect of in vitro NaCl-induced salt stress in cape gooseberry plants and (2) the possible mitigating effect of silicon in saline conditions. For that, nodal segments were inoculated in Murashige and Skoog (MS) medium under salinity (0.5 and 1.0% NaCl) with different silicic acid concentrations (0, 0.5 and 1.0g L-1). Phytotechnical characteristics, photosynthetic pigments content, and leaf anatomy were evaluated after 30 days. Shoot length, root length, number of leaves and buds, fresh and dry weight, pigment content, stomatal density and leaf blade thickness were drastically reduced by increased salt level. The supply of silicon (1.0g L-1) has successfully mitigated the effect of salinity at 0.5% NaCl for chlorophyll, carotenoids, stomatal density and leaf blade thickness. When salt stress was about 1.0%, Si was not effective anymore. In conclusion, we affirmed that, in in vitro conditions, salt stress is harmful for cape gooseberry plants and the addition of silicon showed effective in mitigating the saline effects of some features.

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“…Epstein (2001) concludes that Si accumulates in plants at the same level as that of macronutrients such as calcium, magnesium, and phosphorus. Furthermore, Sahebi et al (2016) reports positive effects of Si on plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Application of silicon sources in yam (Dioscorea spp.) micropropagation

Rodrigues¹,

Rezende²,

Soares³

et al. 2017

Aust J Crop Sci

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The addition of silicon is known to produce satisfactory results in several types of plant cultures. However, there are no reports on the effects of addition of silicon to yam (Dioscorea spp.) micropropagation culture medium. The objective of the present study was to evaluate the effects of different silicon concentrations and sources on the growth characteristics of yam plants cultivated in vitro.Three sources of silicon were tested: potassium silicate, calcium silicate, and sodium silicate, each at concentrations of 0.0, 0.5, 1.0, and 2.0 mg L -1 , in all possible combinations. The experimental design was entirely randomized in a 3 × 4 factorial scheme with four replicates and twelve plants per treatment. The 2 mg L -1 sodium silicate supplement yielded the highest number of leaves (7.0) whereas the 1 mg L -1 sodium silicate produced the longest shoots (4.02 cm). Calcium silicate and potassium silicate yielded the highest fresh weight (0.2835 g and 0.2627 g, respectively). Overall, silicon promotes better plant development, with calcium silicate yielding the highest fresh weight. For in vitro yam cultivation, sodium silicate concentrations ranging between 1-2 mg L -1 are ideal.

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Application of silicon in plant tissue culture

Cited by 34 publications

References 50 publications

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Salt stress and exogenous silicon influence physiological and anatomical features of in vitro-grown cape gooseberry

Application of silicon sources in yam (Dioscorea spp.) micropropagation

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