Application of silicon sources in yam (Dioscorea spp.) micropropagation

Rodrigues, Filipe Almendagna; Rezende, Renata Alves Lara Silva; Soares, Joyce Dória Rodrigues; Rodrigues, Vantuil Antônio; Pasqual, Moacir; Silva, Sebastião de Oliveira e

doi:10.21475/ajcs.17.11.11.pne685

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Therefore, these factors were evaluated separately, showing that Si contributed to an increase in growth characteristics, including LN, RN, PD, and FW (Table 1). Similar to the results obtained in our study, Sivanesan and Park (2014) and Rodrigues et al (2017) demonstrated that Si enhances the growth and development of several species, including an increase in the number of leaves and fresh weight of plants grown in vitro. Particularly for bananas, Si has been shown to increase the height, fresh and dry weight (Asmar et al, 2011), and increased pseudostem diameter (Asmar et al, 2013) of in vitro shoots.…”

Section: Resultssupporting

confidence: 90%

Influence of silicon and in vitro culture systems on the micropropagation and acclimatization of “Dwarf Cavendish” banana

et al. 2020

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In vitro culture systems based on liquid culture media are considered to be more effective than semisolid culture medium systems. Liquid culture media systems provide better nutrient availability for plant tissues, easier culture handling, and the potential for scaling up and automation. However, in vitro liquid culture requires more careful handling due to the potential for contamination and the possibility of negative effects, such as hyperhydricity or vitrification, that hinder the growth and development of the plant material. Temporary immersion bioreactors have emerged as a workable alternative for capturing the benefits of liquid media, though semisolid systems are still traditional. Many studies have shown that silicon (Si) is a beneficial plant nutrient. Silicon might have a positive effect in both semisolid and liquid in vitro systems. The objective of this study was to evaluate the effect of silicon on the micropropagation and acclimatization of banana plants cultivated in vitro by comparing liquid temporary immersion bioreactor technology and semisolid traditional culture systems. Different silicon concentrations (0 and 1 mL L-1) and culture systems (liquid temporary immersion bioreactor and semisolid traditional culture) were evaluated over a 36-day period. The growth characteristics plant size, fresh and dry weight, and number and length of leaves and roots were evaluated. After the 36-day in vitro growth period, plants were transferred to a greenhouse for acclimatization and were evaluated after 30 days for the same growth characteristics used in the in vitro studies. The temporary immersion bioreactor system resulted in greater growth of banana plants compared to the traditional semisolid system. Temporary immersion bioreactors also showed a positive interaction with Si and resulted in higher values for all growth characteristics in the acclimatization phase.

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Section: Resultssupporting

confidence: 90%

Influence of silicon and in vitro culture systems on the micropropagation and acclimatization of “Dwarf Cavendish” banana

et al. 2020

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“…While in the same study (Dias et al, 2017), the highest chlorophyll a and b values were observed in plants with 2.0 mg L -1 sodium silicate added, 1.0 mg L -1 concentration gave higher chlorophyll value in all three silicon sources. Rodrigues et al (2017) in their study in sweet potato with 0.0, 0.5, 1.0 and 2.0 mg L -1 concentrations of potassium, calcium and sodium silicate, the maximum number of leaves (7.0 pieces) was determined. The longest shoots (4.02 cm) were determined from media containing 1 mg L -1 sodium silicate, and the highest plant fresh weights were determined in media containing potassium and calcium silicate (0.263 g and 0.284 g, respectively).…”

Section: Resultsmentioning

confidence: 99%

Effect of Different Silicone Sources and Concentrations on in vitro Micro Propagation of 140 Ru Grape Rootstock

Lateef

Tangolar

2021

International Journal of Agriculture Environment and Food Sciences

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Silicon, which is widely used in different fields, has been used in plant production in vivo and in vitro studies in recent years. Especially in in vitro studies, it is seen that its effect on plant growth and development has been examined. In this study, the effect of three different silicon sources and their four concentrations on micro-propagation of 140 Ru grape rootstocks was investigated. In the study, as explants one-node micro cuttings of rootstock and MS (Murashige and Skoog) as the nutrient medium were used. 1 mg L -1 BA (Benzyl Adenine) at the stage of obtaining shoots from cuttings and in the rooting stage, 1 mg L -1 IBA (Indole Butyric Acid) were added to the nutrient medium. At both stages, 0 (Control), 0.5, 1.0 and 2.0 mg L -1 doses of potassium, sodium and calcium silicate were added to the nutrient medium. Explant viability and mortality rate, shooting rate, plant length, node number, shoot fresh and dry weight, chlorophyll content (SPAD), root number, root length, root fresh and dry weight were examined to determine the effect of the applications. The variance analysis of the study was carried out according to the Two-Way Completely Randomized Experimental Design. According to the results, among the silicon sources, the highest shooting rate (84.40%) was found in the medium containing sodium silicate. The highest shoot fresh and dry weight (0.178 g and 0.026 g, respectively) and root fresh and dry weight values (0.213 g and 0.023 g, respectively) were obtained from potassium silicate. While the number of roots was 2.98 in the medium containing potassium, it was determined as 2.91 in the medium containing calcium silicate. Media containing 1 mg L -1 silicate was found to be more successful than 0, 05, 2 mg L -1 concentrations. The highest values recorded at the concentration were 4.49 cm in plant length, 7.44 in node number, 0.183 g and 0.028 g in shoot fresh and dry weight, respectively, 28.37 in SPAD value and 3.27 in root number. As a result of the study, it is concluded that adding 1 mg L -1 concentration of potassium, calcium and sodium silicate to the nutrient medium can be used in future studies related with in micro propagation.

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“…Lim et al (2012) also reported that the effect of potassium silicate on the growth traits of begonia are mainly dependent on the cultivars. As culture 'GF 677' respond best to the lowest tested concentration (2 mg.l -1 ) while on the control media the plants didn't grow as good, this might suggest that even lower concentrations of silicon, similar to those of which Zhuo (1995) or Rodrigues et al (2017) found as most suitable, could be tested for this cultivar. The decreasing growth parameters with increasing concentrations can indicate lowered tolerance of this genotype to potassium silicate.…”

Section: Variability Of Optimal Concentration Of Silicone In Cultivatmentioning

confidence: 99%

The Effects of Potassium Silicate as a Component of Nutrient Medium for Selected in Vitro Cultures of Prunus and Corylus Genera

Kadlecová

Baránek

Magnus

et al. 2020

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Even though silicon is frequent compound in soil, its use in plant nutrient media is rare. Based on known physiological role and up to now performed studies it seems that silicon has a good potential to improve growth characteristics of in vitro cultivated plants. Before practical application, however, it is always necessary to assess the optimal conditions of application with regard to the fact that plant reactions to different chemicals added to nutritional media can vary on the species or even cultivar level. The presented study evaluate effects of potassium silicate used in in vitro cultivation media on growth parameters of Prunus persica × Prunus davidiana ‘Cadaman,‘ Prunus × amygdalopersica ‘GF 677‘ and Corylus avellana ‘Tonda di Giffoni‘ genotypes, which are frequently subject of commercial in vitro multiplication. In fact, four different concentrations of potassium silicate was added to the multiplication media, control medium was left silicon-free. Three different characteristics were observed during cultivation – number of new shoots per explant, weight of a new plant and length of new shoots, from which number of new shoots per explant was considered the most important factor. In all cases a positive effects of potassium silicate on the condition and other growth parameters of treated cultures were observed. In ‘Cadaman‘ culture significant growth changes appeared on media with 20 mg.l-1 potassium silicate, which can be recommended for future applications. For ‘GF 677‘ the best results were obtained on media with 2 mg.l-1 which can be recommended for improving condition and number of shoots on new plants. For hazelnut genotype ‘Tonda di Giffoni‘ best results were obtained on media with 10 mg.l-1 of potassium silicate, but also use of 5 mg.l-1 of potassium silicate significantly improved growth parameters. Generally, presented study provides important and practically useful insights into the practical use of silicon in cultivation media designated for commercial in vitro micropropagation.

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Application of silicon sources in yam (Dioscorea spp.) micropropagation

Cited by 7 publications

References 22 publications

Influence of silicon and in vitro culture systems on the micropropagation and acclimatization of “Dwarf Cavendish” banana

Influence of silicon and in vitro culture systems on the micropropagation and acclimatization of “Dwarf Cavendish” banana

Effect of Different Silicone Sources and Concentrations on in vitro Micro Propagation of 140 Ru Grape Rootstock

The Effects of Potassium Silicate as a Component of Nutrient Medium for Selected in Vitro Cultures of Prunus and Corylus Genera

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