Application of Rumen Microorganisms for Enhancing Biogas Production of Corn Straw and Livestock Manure in a Pilot-Scale Anaerobic Digestion System: Performance and Microbial Community Analysis

Abstract

This study aimed to assess the feasibility of rumen microorganisms inoculated in a modified pilot-scale system for enhancing biogas production of (1) solely corn straw (CS) and (2) CS with livestock manure under different solid contents and mixture ratios. The biogas liquid was proven to pretreat CS at this scale. The digestion system was started up within 32 days at a retention time of 20 days. The rumen culture was found to have a positive response to the impact on temperature and pH. The optimal solid content of CS was detected to be 3%, resulting in a stable biogas yield of 395 L kg −1 •total solid (TS) −1. A higher biogas yield of 400 L kg −1 •TS −1-420 L kg −1 •TS −1 was achieved at a solid content of 10% organic loading rate (OLR, 4.42 kg volatile solid (VS) m −3 •d −1) in co-digestion systems with CS and livestock manure. The methane content could be maintained at about 60%. Hydrogenotrophic methanogens were dominated by Methanobacterium in the solely CS digestion system, and two methanogenetic pathways, including hydrogenotrophic and acetoclastic methanogens by Methanosarcina and Methanobacterium, co-occurred for methane production during the co-digestion of CS with pig manure (PM). This study indicates that rumen microbes could be utilized in a pilot-scale digestion system and that they greatly promoted the biogas yield.

Effect of sulfonamide pollution on the growth of manure management candidate Hermetia illucens

“…With the rapid growth of the global population and the increasing demands for food from animal origin, industrial livestock and poultry production have expanded significantly in recent decades [1, 2]. The yield of livestock manure reached 2.121 billion tons in 2011, which will continue to increase rapidly to 2.875 billion tons by 2020 and 3.743 billion tons by 2030 [3, 4]. The total amount of pig manure discharge is the first in several livestock manure [3, 4].…”

Anaerobic Digestion Technology for Methane Production Using Deer Manure Under Different Experimental Conditions

“…The results showed municipal sewage sludge as a common inoculum (used in anaerobic fermentation to produce biogas) [57]. Biogas slurry is also an ideal additional source of bacteria [58,59]. Spent mushroom substrate can be used as an important choice in studying fermentation inocula [60].…”

BioEnergy and BioChemicals Production from Biomass and Residual Resources

“…The twenty-one published papers cover a variety of biomass or waste residuals that have been converted into different types of energy, biofuels or biochemicals including heat [1,11,16], methane [2,4,[7][8][9]17,[19][20][21], electricity [2,11,16], short chain fatty acids [2,19], ethanol [3,12,19], syngas [5,19], nutrient pellets [6], hydroxymethylfurfura [15], and hydrogen [2,10,14,18,19]. The key information including biomass or residuals, products, and technology for the production and type of research are summarized in Table 1.…”

“…Jin et al [9] explored the feasibility of using rumen microorganisms as an inoculum to produce biogas from corn straw and livestock manure in a modified pilot-scale anaerobic digestion system under different solid contents and mixture ratios.…”