Application of preparative high-speed counter-current chromatography/electrospray ionization mass spectrometry for a fast screening and fractionation of polyphenols

Solvent system selectivities in countercurrent chromatography using Salicornia gaudichaudiana metabolites as practical example with off-line electrospray mass-spectrometry injection profiling

“…Low detection limits and high identification capability of MS techniques simultaneously resolve compound isolation and identification. In the past, the CCC devices were directly linked in on-line mode to the ESI-and APCI-MS spectrometer [16][17][18][19][20]. A less complicated approach is the off-line injection of HSCCC fractions in the sequence of recovery to determine rapid metabolite profiles [15,21].…”

An application of high-speed counter-current chromatography coupled with electrospray ionization mass spectrometry for separation and online identification of coumarins from Peucedanum praeruptorum Dunn

“…Mass spectrometry characterized as highly sensitive detection was applied in routine analysis, for example, LC/MS and GC/MS. To combine the advantages of the both, analytical HSCCC has been coupled to mass spectrometry with multiple ion sources, such as frit electron ionization (EI), chemical ionization (CI), fast atom bombardment (FAB) and ESI, to analyze chemical components online [8,9]; preparative HSCCC has also been combined with ESI-MS n for separation and identification of polyphenols [10].…”

The Modern Art of Identification of Natural Substances in Whole Plants

“…Yet, online detections are possible using the same detectors as for LC. Thus, the detection can be achieved by UV (including a diode array detector), (Peng et al 2005;Goncalves et al 2011) evaporative light scattering detection (Cao et al 2003;Ha et al 2011) and obviously mass spectrometry with a passive T split (Gutzeit et al 2007) or a variable flow splitter (Toribio et al 2009). …”

“…Many applications are related to flavonoids and other polyphenols (Gutzeit et al 2007;Lu et al 2007;Michel et al 2011b). Among the highest polar metabolites, glycosylated compounds such as glucosinolates (Toribio et al 2007) or saponins (Shirota et al 2008) have also been purified by CPC.…”