Application of polymer-embedded proteins to fabrication of DNA array

Miyachi, Hideaki; Hiratsuka, Atsunori; Ikebukuro, Kazunori; Yano, Kazuyoshi; Muguruma, Hitoshi; Karube, Isao

doi:10.1002/1097-0290(20000805)69:3<323::aid-bit10>3.0.co;2-t

Cited by 65 publications

(47 citation statements)

References 50 publications

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“…Recent studies have confirmed that the QCM-D technique is useful for evaluating surface-related processes in liquids, including protein adsorption, complementary activity on biomaterials, and analysis of DNA hybridization (4, 14,23,26,27). Höök et al reported the effectiveness of the QCM-D technique for analyzing the adsorption kinetics of three model proteins on titanium oxide surfaces compared with ellipsometry and optical waveguide lightmode spectroscopy (14).…”

Section: Discussionmentioning

confidence: 99%

Oxygen plasma surface modification enhances immobilization of simvastatin acid

et al. 2006

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Simvastatin acid (SVA) has been reported to stimulate bone formation with increased expression of BMP-2. Therefore, immobilization of SVA onto dental implants is expected to promote osteogenesis at the bone tissue/implant interface. The aim of this study was to evaluate the immobilization behavior of SVA onto titanium (Ti), O 2 -plasma treated titanium (Ti + O2), thin-film coatings of hexamethyldisiloxane (HMDSO), and O 2 -plasma treated HMDSO (HMDSO + O2) by using the quartz crystal microbalance-dissipation (QCM-D) technique. HMDSO surfaces were activated by the introduction of an OH group and/or O 2 -functional groups by O 2 -plasma treatment. In contrast, titanium surfaces showed no appreciable compositional changes by O 2 -plasma treatment. The QCM-D technique enabled evaluation even at the adsorption behavior of a substance with a low molecular weight such as simvastatin. The largest amount of SVA was adsorbed on O 2 -plasma treated HMDSO surfaces compared to untreated titanium, HMDSO-coated titanium, and O 2 -plasma treated titanium. These findings suggested that the adsorption of SVA was enhanced on more hydrophilic surfaces concomitant with the presence of an OH group and/or O 2 -functional group resulting from the O 2 -plasma treatment, and that an organic film of HMDSO followed by O 2 -plasma treatment is a promising method for the adsorption of SVA in dental implant systems.Simvastatin, one of the liposoluble statins [inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase], has been widely used for lowering cholesterol levels. Recently, simvastatin was reported to stimulate bone formation both in vitro and in vivo in animal models of osteoporosis (10,25). This phenomenon was associated with increased expression of the bone morphogenetic protein-2 (BMP-2) gene in bone cells. Therefore, immobilization of simvastatin onto titanium implants is expected to promote osteogenesis in the bone tissue surrounding the implants through its topical application. Furthermore, one distinct advantage of simvastatin for local stimulation of bone formation is its low manufacturing cost compared to the direct administration of recombinant proteins such as BMPs. There is also a reduced possibility of eliciting antibody responses.Plasma-surface modification including plasma polymerization and plasma surface treatment is an effective and economical surface treatment technique for many materials. The technique is of growing interest in biomedical engineering because it can control the physicochemical properties of biomaterials

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Section: Discussionmentioning

confidence: 99%

Oxygen plasma surface modification enhances immobilization of simvastatin acid

et al. 2006

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“…13 The properties of the PPF film include (a) the ability to be made extremely thin (< 1 µm), (b) the good adhesion to any substrate, (c) the pin-holefree and flat surface structure, (d) the mechanical and chemical stability (a result of its highly-branched and cross-linked structure), and (e) the capability to accommodate a large amount of biomaterials, such as enzymes, 14 antibodies, 15 and oligonucleotides 16 which can be immobilized onto the surface of the film. We propose a new strategy for the fabrication of mediator biosensor, based upon PPF.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Kase

Muguruma

2004

ANAL. SCI.

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We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 -81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

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“…Plasma surface modifi cation including plasma polymerization and plasma surface treatment is an effective and economical method of surface treatment for many materials. The method is of growing interest in biomedical engineering because it can be used to control the physicochemical properties of biomaterials (3,17,18). Plasma surface treatments using various gases such as Ar, O 2 , N 2 , and SO 2 have been employed to modify blood compatibility, to infl uence cell adhesion and growth, and to control protein adsorption (2,8,21,22,26).…”

mentioning

confidence: 99%

Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surface

Matsuzaka¹,

Yoshinari²,

Kokubu³

et al. 2004

Biomed. Res.

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Property modifi cation of an implant surface is known to infl uence the behavior of cells surrounding implant material. This study examined the osteogenic cell behavior on protein immobilized surface in vitro. Following plasma surface modifi cation, fi bronectin or bone morphogenetic protein-2 (BMP-2) was immobilized. The number of cells adhering to fi bronectin-immobilized surface increased after 1 and 2 h of incubation compared with non-immobilized surface. Alkaline phosphatase activity and osteocalcin mRNA expression of the osteogenic cells on the BMP-2 immobilized surface was greater than that on the non-immobilized surface. This study demonstrated that protein can be immobilized to a polystyrene surface after treatment with O 2 plasma and that the osteogenic cells surrounding a biomaterial can be controlled by the immobilization of protein to the biomaterial.

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Application of polymer-embedded proteins to fabrication of DNA array

Cited by 65 publications

References 50 publications

Oxygen plasma surface modification enhances immobilization of simvastatin acid

Oxygen plasma surface modification enhances immobilization of simvastatin acid

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surface

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