Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Abstract

We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 -81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

The effect of the layer structure on the activity of immobilized enzymes in ultrathin films

“…The catalytic activity of GOD was also estimated by measuring the increase in current in the voltammograms after adding aliquots of glucose at a fixed potential of 0.43 V vs Ag/AgCl, which is close to the potential used in other GOD-based biosensors [31]. All the electrochemical measurements were performed at room temperature (23 • C).…”

Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film

“…Their applications are as enzyme amperomertric biosensors Kase and Muguruma, 2004), quartz crystal microbalance (QCM) biosensors (Wang et al, 2004a,b;Nakanishi et al, 1996) and surface plasmon resonance biosensors Nakamura et al, 1997). There are also advantages for fabrication strategy such as BioMEMS process (Bouaidat et al, 2004;Hanein et al, 2001;Hiratsuka et al, 2004) and inside wall coating for capillary electrophoresis Loughran et al, 2003).…”

Fluorometric Method for Quantitative Determination of Glucose and Its Application to Human Serum

“…14 Despite all that, the reagents which exhibit characteristic luminescence while oxidized by hydrogen peroxide are difficult to find, so their application is limited. Electrochemical methods for the determination of glucose are widely used 15 because of their high sensitivity and rapidity. However, they are expensive and easily suffer from some reductive substances such as vitamin C and uric acid.…”