Application of PLE for the determination of essential oil components from Thymus vulgaris L.

Dawidowicz, Andrzej L.; Rado, Ewelina; Wianowska, Dorota; Mardarowicz, Marek; Gawdzik, J.

doi:10.1016/j.talanta.2008.04.050

Cited by 80 publications

(47 citation statements)

References 16 publications

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“…This is, for instance, the case of the extraction of volatiles and essential oils that are more efficiently extracted with solvents such as nhexane [82][83][84]. However, as it has been already mentioned, one of the most important trends in this field is based on the search and application of new environmentally green and food-grade solvents.…”

Section: Methodological and Technological Advancesmentioning

confidence: 99%

Compressed fluids for the extraction of bioactive compounds

Herrero

Castro-Puyana

Mendiola

et al. 2013

TrAC Trends in Analytical Chemistry

305

150

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ABSTRACT.The improvement of sample preparation and extraction techniques for natural bioactive compounds determinations is of great importance nowadays. New concepts are being introduced in this field, not only related to enhancement of extraction efficiencies but also related to environmental impact. This evolution towards Green Chemistry is pushing to new extraction and sample preparation processes that should be faster, more reproducible and more environmentally friendly. In this context, compressed fluidsbased sample preparation techniques (such as supercritical fluid extraction and pressurized liquid extraction) are demonstrating good capabilities. In this review, an updated knowledge on the techniques will be presented together with the main technical developments and the most notable recent applications for the extraction of bioactive compounds.Keywords: bioactive compounds, natural samples, plants, agricultural by-products, pressurized liquid extraction, supercritical fluid extraction, subcritical water extraction Abbreviations: ASE, accelerated solvent extraction; DHA, docosahexaenoic acid; EAE, enzyme-assisted extraction; EPA, eicosapentaenoic acid; IL, ionic liquids; MIP, molecular imprinted polymers; PFE, pressurized fluid extraction; PHSE, pressurized hot solvent extraction; PHWE, pressurized hot water extraction; PLE, pressurized liquid extraction; PLPW, pressurized low polarity water extraction; n-3 PUFA, n-3 polyunsaturated fatty acid; RAM, restricted access media; scCO2, supercritical CO2; SFE, supercritical fluid extraction; SHWE, superheated water extraction; SPE, solid Published in TrAC. Trends in analytical chemistry [0165-99362012 vol:43 pg:67 -83 http://dx.doi.org/10.1016/j.trac.2012.12.008 3 phase extraction; SWE, subcritical water extraction; TG, triglycerides; UAE, ultrasound assisted extraction; WEPO, water extraction and particle formation on-line.

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Section: Methodological and Technological Advancesmentioning

confidence: 99%

Compressed fluids for the extraction of bioactive compounds

Herrero

Castro-Puyana

Mendiola

et al. 2013

TrAC Trends in Analytical Chemistry

305

150

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“…The same differences have been reported during the analysis of other plants using both methods, which in turn raised the question about the true nature of the volatile compounds of the analyzed herbs (Drew et al, 2012). Dawidowicz et al (2008) attributed the differences in the components of the essential oil of Thymus vulgaris obtained by hydrodistillation and those found by HS-SPME were due to the nature of the fiber. In our case this explanation can be ruled out because different types of fibers were employed.…”

Section: Discussionmentioning

confidence: 64%

Quality control tests for the crude drug ofConyza filaginoides

Ovalle-Magallanes

Rivero‐Cruz

Mata

2013

Pharmaceutical Biology

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Context: Conyza filaginoides (D.C.) Hieron (Asteraceae) is a medicinal Mexican plant highly prized in contemporary Mexico for the treatment of upset stomach and diabetes. Objective: This work was undertaken to develop a suitable high performance liquid chromatography (HPLC)-diode array detection (DAD) method for quantifying rutin (1), the main active principle from the aerial parts of C. filaginoides. Materials and methods: The method was performed using a LiChrospher 100 RP-18 column. The mobile phase was water (containing 0.1% phosphoric acid)-methanol-acetonitrile (80:5:15, v/v) at a flow rate of 1.2 mL min À1. Results: Limits of detection and quantification were 7.5 and 22.8 mg mL À1, respectively. The main recoveries measured at three concentrations were higher than 98%, with RSD 52%. Quantitative analysis of a few samples showed the presence of high concentrations of 1 (3.6 AE 0.2 g/100 g of dry plant material). The volatile components were extracted by hydrodistillation or head space solid-phase microextraction (HS-SPME), and thereafter analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Forty-three chemical constituents representing 90% of the total content of the oil were identified. The major light volatile compounds obtained by HS-SPME revealed a high content of monoterpene hydrocarbons.Conclusions: A precise, reliable, and accurate analytical HPLC method to detect and quantify 1 in the crude drug and some preparations were developed and fully validated. The volatile components of the plant are described for the first time. The proposed method would be useful for quality control assurance of this important Mexican plant.

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“…Even if in the previous literature data, the ratio of thymol in the headspace was much lower than in the distilled essential oil (Venskutonis, 1997;Dawidowicz et al, 2008;Bertoli et al, 2010); the authors underlined that the sampling method should have been optimized. Indeed, according to our results the thymol ratios almost reach that one measured in the distilled oil in two cases (crumbled drug, incubation time 30 min, temperature 25 and 60°C).…”

Section: Solid Phase Microextraction (Spme)mentioning

confidence: 92%

“…1 h equilibration time at 60 C was applied by Bicchi et al (2000) using 0.6 g plant material hermetically sealed in a 12.5 ml vial. In the research work of Dawidowicz et al (2008) the fiber was introduced into a thermostated vial (at 25°C) for 30 min, using 2.0 g dried foliage. However, real optimization of the above mentioned parameters have not been carried out yet; therefore we initiated an analytic work in this field using 0.6 g dried plant material (grounded and crumbled); our first results can be seen in Table 5.…”

Section: Solid Phase Microextraction (Spme)mentioning

confidence: 99%

Effects of different factors influencing the essential oil properties of Thymus vulgaris L.

2016

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Thymus vulgaris L. is a well-know medicinal and aromatic plant native to the Mediterranean region. The essential oil is considered as the main active constituent, being responsible for its typical odour and taste as well as for several therapeutic effects. Our aim was to demonstrate the most important factors influencing the quality and quantity parameters of thyme oil by summarizing the available literature data and our own scientific results. Genetic background, climatic and growing conditions, techniques of primary processing, storage conditions as well as different extraction methods have proven effects on the essential oil properties and, as a consequence, on its biological activity, either.

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Application of PLE for the determination of essential oil components from Thymus vulgaris L.

Cited by 80 publications

References 16 publications

Compressed fluids for the extraction of bioactive compounds

Compressed fluids for the extraction of bioactive compounds

Quality control tests for the crude drug ofConyza filaginoides

Effects of different factors influencing the essential oil properties of Thymus vulgaris L.

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