Application of peroxidase labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses

Afshar, Abbas Aghaei; Dulac, G C; Bouffard, Alice

doi:10.1016/0166-0934(89)90158-4

Cited by 36 publications

(15 citation statements)

References 8 publications

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“…The method for this assay was adapted from Afshar et al (1989). One ml of allantoic fluid sample was filtered through a 0.45-/im filter, and then diluted 1:10 in Earle's minimum essential medium supplemented with 20% foetal bovine serum and 50 /<g/ml gentamycin (MEM).…”

Section: Indirect Immunoperoxidase Assay (Ipa)mentioning

confidence: 99%

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

et al. 1998

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Section: Indirect Immunoperoxidase Assay (Ipa)mentioning

confidence: 99%

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

et al. 1998

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“…The infectivity assay was performed by Peroxidase Linked Assay (PLA) as described by AFSHAR et al (1989). Briefly, 50 µl of double serial dilutions of virus samples were diluted in growth medium and distributed on the microtitre plate.…”

Section: Virus Titrationmentioning

confidence: 99%

Effect of prostaglandin A₁ in porcine cells persistently infected with classical swine fever virus

Freitas

Caldas

Rebello

2003

J. Basic Microbiol.

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Cyclopentenone prostaglandins are potent inhibitors of a wide variety of RNA and DNA viruses. In this report we describe that prostaglandin A1 (PGA1) potently inhibited the replication of classical swine fever virus in cultures of PK-15 cells. The highest non-toxic dose (5 microg/ml) inhibited virus yield in 99% at the initial phase of infection and in 77% in persistent infected cells. However when PGA1 was removed from persistently infected cells, the inhibition of virus replication was partially reverted.

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“…The PLA was performed based in Afshar et al (1989) and Office International des Epizooties -OIE Manual of Standards (1992) in microtitre plates and was standardized as described. 50 µl of double serial dilutions of virus samples were diluted in growth medium and distributed on the microtitre plate.…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin A1 Inhibits Replication of Classical Swine Fever Virus

Freitas¹,

Caldas²,

Rebello

1998

Mem. Inst. Oswaldo Cruz

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Prostaglandins (Pgs) have been shown to inhibit the replication of several DNA and RNA viruses. Here we report the effect of prostaglandin (PgA1) on the multiplication of a positive strand RNA virus, Classical Swine Fever Virus (CSFV) in PK15 cells. PgA1 was found to inhibit the multiplication of CSFV. At a concentration of 5 µg/ml, which was nontoxic to the cells, PgA1 inhibitis virus production in 99%. In PgA1 treated cells the size and number of characteristic Classical Swine Fever focus decreased in amount

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Application of peroxidase labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses

Cited by 36 publications

References 8 publications

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

Effect of prostaglandin A₁ in porcine cells persistently infected with classical swine fever virus

Prostaglandin A1 Inhibits Replication of Classical Swine Fever Virus

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Application of peroxidase labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses

Cited by 36 publications

References 8 publications

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

Excretion of pathogenic Newcastle disease virus by double‐crested cormorants (Phalacrocorax auritus) in absence of mortality or clinical signs of disease

Effect of prostaglandin A1 in porcine cells persistently infected with classical swine fever virus

Prostaglandin A1 Inhibits Replication of Classical Swine Fever Virus

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Effect of prostaglandin A₁ in porcine cells persistently infected with classical swine fever virus