Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs

Akizuki, Tanetaka; Oda, Shigeru; Komaki, Motohiro; Tsuchioka, Hiroaki; Kawakatsu, N.; Kikuchi, Akihiko; Yamato, Masayuki; Okano, Teruo; Ishikawa, Isao

doi:10.1111/j.1600-0765.2005.00799.x

Cited by 260 publications

(227 citation statements)

References 27 publications

(33 reference statements)

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“…Akizuki et al (19) investigated periodontal healing in beagle dogs following application of a PDL cell sheet, which was prepared using temperature-responsive cell culture. Dehiscence defects were surgically created on the buccal surface of the mesial roots of bilateral mandibular first molars in each dog.…”

Section: Applications Of Cell Sheet Techniques With Non-mesenchymal Smentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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Section: Applications Of Cell Sheet Techniques With Non-mesenchymal Smentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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“…This approach has been successfully used to promote periodontal regeneration in a number of small and large animal models (Akizuki et al, 2005;Flores et al, 2008;Ishikawa et al, 2009;Vaquette et al, 2012;Costa et al, 2014).…”

mentioning

confidence: 99%

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

et al. 2014

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The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with NH 4 OH/Triton X-100 and DNase solutions. The protocol was shown to remove 92% of DNA content. The structural integrity of the decellularized cell sheets was confirmed by a collagen quantification assay, immunostaining of human collagen type I and fibronectin, and scanning electron microscopy. ELISA was used to demonstrate the presence of residual basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) in the decellularized cell sheet constructs. The decellularized cell sheets were shown to have the ability to support recellularization by allogenic human periodontal ligament cells. This study describes the fabrication of decellularized periodontal ligament cell sheets that retain an intact extracellular matrix and resident growth factors and can support repopulation by allogenic cells. The decellularized hPDL cell sheet concept has the potential to be utilized in future "off-theshelf " periodontal tissue engineering strategies.

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“…Os animais foram dissecados para remoção dos incisivos em uma câmara de fluxo laminar e sob condições assépticas, segundo protocolo estabelecido 18 . Os dentes foram imediatamente mantidos em um meio básico α-MEM contendo 50 mg/L de Sulfato de Gentamicina e 2 mg/L de Anfotericina B (Cultilab, Brasil).…”

Section: Obtenção E Cultivo Das Células Do Ligamento Periodontal De Runclassified

Adesão de Células do Ligamento Periodontal de Ratos a Diferentes Superfícies de Titânio: Estudo Comparativo In Vitro

ALVES¹

2011

Pesqui. bras. odontopediatria clín. integr.

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Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs

Abstract: The periodontal ligament cell sheet has a potential to regenerate periodontal tissue and may become a novel regenerative therapy.

Cited by 260 publications

References 27 publications

Application of the cell sheet technique in tissue engineering

Application of the cell sheet technique in tissue engineering

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

Adesão de Células do Ligamento Periodontal de Ratos a Diferentes Superfícies de Titânio: Estudo Comparativo In Vitro

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