Application of Nanomaterials in Stem Cells, Tissue Engineering and Regenerative Medicine

Hollands, Peter

doi:10.1007/978-981-15-4802-4_5

Cited by 1 publication

(1 citation statement)

References 128 publications

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“…Effective tissue engineering requires a suitable stem cell source. In this regard, adult mesenchymal stem cells (MSCs) offer new insights into the progress of therapeutic strategies for different types of diseases (cardiovascular [ 30 ] and neurodegenerative [ 31 ]) and the development of a biohybrid system for tissue engineering applications [ 32 , 33 , 34 ]. Multipotent MSCs can differentiate towards osteogenic, adipogenic, chondrogenic, or neural lineages [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

et al. 2021

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The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

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