International private umbilical cord blood banking has expanded rapidly in recent years since the first cord blood transplant which was 20 years ago. Private companies offer parents the opportunity to store umbilical cord blood for the possible future use by their child or other family members. The private cord blood industry has been criticised by a number of professional bodies including the EU Ethics Committee, the Royal College of Obstetrics and Gynaecology, the Royal College of Midwives and the US College of Paediatrics. This review presents the arguments from the opponents of private cord blood banking, and then makes the case for private cord banking based on the latest scientific and clinical evidence.
The diagnosis of genetic disease in preimplantation embryos is discussed. The typing of spermatozoa may be feasible for factors such as the presence of an X and Y chromosome. Embryos might be typed by non-invasive methods, by assessing their uptake of metabolites although the widest opportunities may arise by the use of invasive methods which involve the removal of one or a small number of cells. The methods of diagnosis are discussed, including enzyme assays and the use of DNA probes, preliminary results with human embryos are presented and the difficulties related to these techniques are debated. The low rate of implantation of replaced embryos will mean that many embryos will have to be diagnosed, and certain embryological factors such as the high incidence of chromosomal imbalance and the problems of 'imprinting' might obscure certain diagnoses. The advantages and disadvantages of the method are discussed.
The mesenchymal stem cells (dental pulp stem cells; DPSC) found inside teeth represent a significant future source of stem cells for regenerative medicine procedures. This review describes the ontogeny of DPSC; the laboratory processing and collection of DPSC; the immuno-cytochemical characterisation of DPSC; the differentiation between adult DPSC and DPSC obtained from exfoliated deciduous teeth (SHED) and their potential use in regenerative medicine procedures in the future both in dental and general medical applications.
There are now various sources of stem cells. Those derived from blastocysts, named embryo stem (ES) cells, have attracted most attention and are highly multipotent. Human cord blood became widely used as a source of stem cells with differing properties to ES cells and their therapeutic application has grown steadily as they are stored in increasing numbers of stem cell banks. Other sources of human stem cells are derived from peripheral blood and amniotic fluid. They may arise from a common origin in epiblast. This review stresses the use of cord blood stem cells, but describes new approaches which may supersede the use of most stem cells. The advantages and disadvantages of these various classes are described in relation to potential methods involving gene conversion to change somatic cells to ES cells.
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