“…Immunofluorescences were performed according to our previous work [ 28 , 29 , 30 ]. Briefly, cells seeded on glass coverslips at the optimal density of 1 × 10 3 were fixed with 4% paraformaldehyde, permeabilized, blocked in PBS + 10% FBS, 0.1% Triton X-100 for 1 h at RT, and incubated overnight at 4 °C with the primary antibody, that based on the analyses were: anti-collagen I (COL.1) (1:300, Chemicon International, Temecula, CA, USA) and anti-collagen III (1:300, COL.3 Chemicon International, Temecula, CA, USA); anti-SOD1 (1:400, Santa Cruz Biotechnology, CA, USA).…”