Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

Abstract

The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

Interfacial Compatibilization into PLA/Mg Composites for Improved In Vitro Bioactivity and Stem Cell Adhesion

“…More experiments are necessary to explain the mechanism. In conclusion, in accordance with our previous works, all these data confirmed that the two types of stem cells used exhibit a different interaction and behaviour on the same film surface [ 57 , 72 ].…”

Hyaluronic Acid-Functionalized Mesoporous Silica Nanoparticles Loading Simvastatin for Targeted Therapy of Atherosclerosis

“…To obtain FITC-labeled MSN, amino-conjugated nanoparticles (NH 2 -MSN) were synthesized according to the literature [33]. Briefly, 100 mg MSN was dispersed in 50 mL methanol after 30 min of ultrasound treatment.…”

Storage of Mutant Human SOD1 in Non-Neural Cells from the Type-1 Amyotrophic Lateral Sclerosis ratG93A Model Correlated with the Lysosomes’ Dysfunction

“…Immunofluorescences were performed according to our previous work [ 28 , 29 , 30 ]. Briefly, cells seeded on glass coverslips at the optimal density of 1 × 10 3 were fixed with 4% paraformaldehyde, permeabilized, blocked in PBS + 10% FBS, 0.1% Triton X-100 for 1 h at RT, and incubated overnight at 4 °C with the primary antibody, that based on the analyses were: anti-collagen I (COL.1) (1:300, Chemicon International, Temecula, CA, USA) and anti-collagen III (1:300, COL.3 Chemicon International, Temecula, CA, USA); anti-SOD1 (1:400, Santa Cruz Biotechnology, CA, USA).…”