2002
DOI: 10.1016/s0378-1135(02)00097-4
|View full text |Cite
|
Sign up to set email alerts
|

Application of N-PCR for diagnosis of distemper in dogs and fur animals

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
15
0
1

Year Published

2003
2003
2018
2018

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 33 publications
(19 citation statements)
references
References 9 publications
2
15
0
1
Order By: Relevance
“…The sensitivity of RT-PCR alone was inadequate enough to detect viral RNA. This work co related with the superiority of nested PCR as a RNA detection tool by Rzezutka et al, 2002 and falls in line to the higher sensitivity of detection as stated as Yong-Hwan Kim et al, 2001. Non-specific reactions were common in conventional antigen detection methods such as AGPT and DOT-ELISA.…”
Section: Nested Pcr Combined With Rt Pcrmentioning
confidence: 59%
“…The sensitivity of RT-PCR alone was inadequate enough to detect viral RNA. This work co related with the superiority of nested PCR as a RNA detection tool by Rzezutka et al, 2002 and falls in line to the higher sensitivity of detection as stated as Yong-Hwan Kim et al, 2001. Non-specific reactions were common in conventional antigen detection methods such as AGPT and DOT-ELISA.…”
Section: Nested Pcr Combined With Rt Pcrmentioning
confidence: 59%
“…The application of molecular techniques (for example, gel-based RT-PCR) in CDV diagnosis has been successfully used in different types of samples collected from animals clinically suspected of infection (Frisk et al 1999, Kim et al 2001, Rzezutka & Mizak 2002, Gebara et al 2004. It is a specific, practical and rapid tool in CDV diagnosis ante mortem considering the non-specific clinical signs of the disease, but it is not a quantitative and very sensitive assay.…”
Section: Discussionmentioning
confidence: 99%
“…ELISA assay may detect high antibody titers to CDV for several months after vaccination or after subclinical or clinical infection, turning false positive results (Martella et al 2008). Molecular assays, such as RT-PCR (Frisk et al 1999, Kim et al 2001, Rzezutka & Mizak 2002, Gebara et al 2004) are sensitive and specific, but not quantitative.…”
Section: Canine Distemper Virus Detection In Asymptomatic Andmentioning
confidence: 99%
“…CDV immunoassays for detection of CDV antigen, such as immunofluorescence staining and ELISA [28][29][30][31][32], in general are not sensitive enough to detect CDV in asymptomatic dogs or during acute CD infection [31]. Various conventional, nested, and real-time reverse transcription polymerase chain reaction (RT-PCR) assays, which offer high degrees of detection sensitivity and specificity, have also been developed for CDV [33][34][35][36][37][38][39][40][41][42]. However, requiring an experienced technician and a relatively expensive thermocycler, these assays have been applied to CDV diagnosis mostly in professional laboratories.…”
Section: Introductionmentioning
confidence: 99%