2010
DOI: 10.1590/s0100-736x2010000200007
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Abstract: Samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. Canine distemper virus vaccine samples in VERO cells were used as positive controls. RNA was isolated with Trizol®, and treated with a TURBO DNA-free kit. Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). Canine β-actin (93 bp) was utilized as the endogenous control for normalization. Quantitative results of real time PCR … Show more

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Cited by 10 publications
(3 citation statements)
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“…During experimental infection of sheep and goats, virus excretion has been demonstrated for animals with limited clinical signs [ 80 , 81 ], and it is likely that under field conditions, animals with very mild clinical signs play a role in virus transmission and maintenance. Transmission by sub-clinically infected dogs has been demonstrated for canine distemper virus, another morbillivirus [ 82 ]. Therefore, it is possible that some wild species could develop mild clinical signs and transmit a virus and, if there is an adequate supply of naïve animals to be infected, they could play a role as a bridge or maintenance host.…”
Section: Discussionmentioning
confidence: 99%
“…During experimental infection of sheep and goats, virus excretion has been demonstrated for animals with limited clinical signs [ 80 , 81 ], and it is likely that under field conditions, animals with very mild clinical signs play a role in virus transmission and maintenance. Transmission by sub-clinically infected dogs has been demonstrated for canine distemper virus, another morbillivirus [ 82 ]. Therefore, it is possible that some wild species could develop mild clinical signs and transmit a virus and, if there is an adequate supply of naïve animals to be infected, they could play a role as a bridge or maintenance host.…”
Section: Discussionmentioning
confidence: 99%
“…An ExicyclerTM 96 Real-Time Quantitative Thermal Block (Bioneer) system was used to amplify cDNA using both the designed (CDVF: 5’-CAC CTT CTA CAA CGA GCT GCG-3’ and CDVR: 5’-ATC TTC TCA CGG TTG GCC TTG-3’) and provided primer (Macrogen, Korea) of the NP gene and housekeeping gene b-actin [ 16 ].…”
Section: Methodsmentioning
confidence: 99%
“…Pleomórfico e com diâmetro variável entre 150 e 250 nm, o VCC apresenta como material genético uma fita simples de RNA de cadeia negativa constituído de aproximadamente 16 kb. 5,10 O VCC (Figura 1) possui 8 proteínas sendo duas não estruturais (C e V) e seis proteínas estruturais: NP (proteína do nucleocapsídeo), P (fosfoproteína), M (proteína da matriz), F (proteína de fusão), H (hemaglutinina) e L (grande proteína).…”
Section: Figura 10unclassified