2010
DOI: 10.1590/s0100-736x2010000200007
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Canine distemper virus detection in asymptomatic and non vaccinated dogs

Abstract: Samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. Canine distemper virus vaccine samples in VERO cells were used as positive controls. RNA was isolated with Trizol®, and treated with a TURBO DNA-free kit. Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). Canine β-actin (93 bp) was utilized as the endogenous control for normalization. Quantitative results of real time PCR … Show more

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Cited by 11 publications
(5 citation statements)
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References 21 publications
(25 reference statements)
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“…A vacina antiviral polivalente é essencial para os animais, sendo que ela deve ser fornecida aos animais desde filhotes, com reforço anual (FIGHERA et al, 2008;DEL PUERTO, 2010). A vacina citada previne doenças virais e bacterianas em cães e gatos, sendo que em cães previne contra cinomose, parvovirose, coronavirose, leptospirose, dentre outras.…”
Section: Resultsunclassified
“…A vacina antiviral polivalente é essencial para os animais, sendo que ela deve ser fornecida aos animais desde filhotes, com reforço anual (FIGHERA et al, 2008;DEL PUERTO, 2010). A vacina citada previne doenças virais e bacterianas em cães e gatos, sendo que em cães previne contra cinomose, parvovirose, coronavirose, leptospirose, dentre outras.…”
Section: Resultsunclassified
“…However, vaccination coverage may vary greatly between different countries and regions although most countries provide CD vaccination. In places with insufficient veterinary service, vaccination rates may be low, and stray dogs in these areas may not receive adequate vaccination protection against CDV which are susceptible infected in CDV (Del et al 2010 ). By the same reason, the prevalence rate in non-vaccinated dogs is under report.…”
Section: Discussionmentioning
confidence: 99%
“…During experimental infection of sheep and goats, virus excretion has been demonstrated for animals with limited clinical signs [ 80 , 81 ], and it is likely that under field conditions, animals with very mild clinical signs play a role in virus transmission and maintenance. Transmission by sub-clinically infected dogs has been demonstrated for canine distemper virus, another morbillivirus [ 82 ]. Therefore, it is possible that some wild species could develop mild clinical signs and transmit a virus and, if there is an adequate supply of naïve animals to be infected, they could play a role as a bridge or maintenance host.…”
Section: Discussionmentioning
confidence: 99%