2008
DOI: 10.18000/ijabeg.10024
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Detection Of Canine Distemper Virus Using Nested PCR

Abstract: Canine Distemper Virus is one of the most contagious RNA virus, which causes many fatal respiratory problems in dogs, seals, Red foxes, lions, badgers etc.,. Detection of CDV poses a very big and important task among veterinarians. This report mainly emphasis on the isolation and detection of the canine distemper virus in a novel way. Nasal and ocular samples from 25 dogs were collected from the clinical ward. Out of that, the first 5 were cultured in cell lines, namely MDCK and VERO. These cultured samples we… Show more

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“…From clinical cases, the virus can be isolated from peripheral blood leukocytes, cerebrospinal fluid and brain samples in B95a (Mori et al, 1994), Vero, MDCK (Muthiah et al, 2008) and MV 1 Lu cells (Lednicky et al, 2004). Further, the virus can be detected using serological tests like ELISA and VNT (Twark and Dodds, 2000), molecular tests like amplification of N, F or H gene by RT-PCR followed by DNA sequencing (Ashmi et al, 2017;Mira et al, 2018) and qRT-PCR (Elia et al, 2006;Scagliarini et al, 2007); along with other techniques like direct FAT, immunohistochemistry and in situ hybridisation (Maes et al, 2003).…”
Section: Introductionmentioning
confidence: 99%
“…From clinical cases, the virus can be isolated from peripheral blood leukocytes, cerebrospinal fluid and brain samples in B95a (Mori et al, 1994), Vero, MDCK (Muthiah et al, 2008) and MV 1 Lu cells (Lednicky et al, 2004). Further, the virus can be detected using serological tests like ELISA and VNT (Twark and Dodds, 2000), molecular tests like amplification of N, F or H gene by RT-PCR followed by DNA sequencing (Ashmi et al, 2017;Mira et al, 2018) and qRT-PCR (Elia et al, 2006;Scagliarini et al, 2007); along with other techniques like direct FAT, immunohistochemistry and in situ hybridisation (Maes et al, 2003).…”
Section: Introductionmentioning
confidence: 99%