Detection Of Canine Distemper Virus Using Nested PCR

Abstract

Canine Distemper Virus is one of the most contagious RNA virus, which causes many fatal respiratory problems in dogs, seals, Red foxes, lions, badgers etc.,. Detection of CDV poses a very big and important task among veterinarians. This report mainly emphasis on the isolation and detection of the canine distemper virus in a novel way. Nasal and ocular samples from 25 dogs were collected from the clinical ward. Out of that, the first 5 were cultured in cell lines, namely MDCK and VERO. These cultured samples were passaged each for about 7 times which was sufficient enough for PCR detection. Field samples and cultured samples were then processed. The Viral RNA was extracted from cultured samples and the corresponding cDNAs were synthesized. Finally RT-PCR was done and it was followed by nested PCR. Out of the 25 field samples tested, 2 of them were detected positive and out of the 5 cultured samples, 3 were detected positive. Thus, the sensitivity of detection is shown to increase by about 40% when cultured, as the RNA content is prone to increase in cell culture.ergillus niger, Surface culture, Banana peel, Biomass, Fermentation.

H Gene-based Molecular Characterization of Field Isolates of Canine Distemper Virus from Cases of Canine Gastroenteritis

“…From clinical cases, the virus can be isolated from peripheral blood leukocytes, cerebrospinal fluid and brain samples in B95a (Mori et al, 1994), Vero, MDCK (Muthiah et al, 2008) and MV 1 Lu cells (Lednicky et al, 2004). Further, the virus can be detected using serological tests like ELISA and VNT (Twark and Dodds, 2000), molecular tests like amplification of N, F or H gene by RT-PCR followed by DNA sequencing (Ashmi et al, 2017;Mira et al, 2018) and qRT-PCR (Elia et al, 2006;Scagliarini et al, 2007); along with other techniques like direct FAT, immunohistochemistry and in situ hybridisation (Maes et al, 2003).…”