Application of HS-SPME in the determination of potentially toxic organic compounds emitted from resin-based dental materials

Abstract

Leaching of volatile substances from resin-based dental materials may have a potential impact on the biocompatibility as well as safety of these materials. Information from manufacturers on ingredients in the materials is very often incomplete. Patients and dentists may be in contact with components emitted from cured dental fillings or from substrates applied in their preparation. Therefore, determination of the components of these materials is necessary for better prevention from possible harmful effects caused by dental fillings. The aim of this work was the isolation and identification of organic compounds evolved from four commercial resin-modified glass-ionomer cements (resin-based dental materials applied in dentistry) by using an alternative method of volatile compounds analysis-HS-SPME (headspace-solid phase microextraction). Dental materials were heated in closed vial at various temperatures and volatile substances released into the headspace phase above the sample were isolated on a thin polymeric fibre placed in SPME syringe. Identification was performed by using the GC-MS (gas chromatography-mass spectrometry) technique. Almost 50 RMGIC (resin-modified glass-ionomer cement) components (monomers and additives) were identified. The main identified leachables were: iodobenzene (DPICls-diphenyliodonium chloride degradation product), camphorquinone (photo-initiator), tert-butyl-p-hydroxyanisole (inhibitor), 4-(dimethylamino)ethyl benzoate (co-initiator), ethylene glycol dimethacrylate (monomer).

Analysis of organic components in resin‐modified pulp capping materials: critical considerations

“…Safety data sheets have been reported as incomplete for many products, including PRMs . The same was evident for the materials investigated in the present study.…”

Determination of the optimal photoinitiator concentration in dental composites based on essential material properties

“…This is a concern because photoinitiating systems are increasingly being used in the field of tissue engineering where the materials are in intimate contact with highly vascularized tissues [23 – 25]. There has been recent confirmation of the possibility of CQ exposure from resin-based materials [26], evidence of potential cytotoxicity of leached CQ molecules [27, 28]. This underscores the need for cautious formulation of materials with the intention to eliminate or, at least, significantly minimize CQ exposure to biological tissues.…”

Release and toxicity of dental resin composite

“…The possible routes of systemic intake of chemical substances released from resin based composites can be through (i) oral mucosa directly, (ii) diffusion to pulp via dentinal tubules,[2] (iii) absorption of volatile components in lungs[67] and (iv) ingestion of released components in the gastrointestinal tract. [8]…”