Application of HPLC coupled with DAD, APcI-MS and NMR to the analysis of lutein and zeaxanthin stereoisomers in thermally processed vegetables

Aman, Robert; Biehl, J; Carle, Reinhold; Conrad, Jürgen; Beifuß, Uwe; Schieber, Andreas

doi:10.1016/j.foodchem.2004.10.031

Cited by 118 publications

(101 citation statements)

References 34 publications

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“…10C-E). Rather, unidentified peaks are likely isomers of the xanthophylls identified in previous studies using the same HPLC conditions (30,34). HPLC chromatogram profiles are similar to a study using micelles to deliver lutein and zeaxanthin to differentiated ARPE-19 cells (30).…”

Section: Carotenoids Are Not Extensively Metabolized By Arpe-19 Cellssupporting

confidence: 70%

“…Lutein, zeaxanthin, and -carotene were analyzed using an Agilent Technologies 1200 series diode array and multiple wavelength detector HPLC system (Santa Clara, CA) using a method previously described (30,34). A column C30 type carotenoid, 4.6 × 250 mm, 3 m (YMC, Inc., Milford, MA) was used with methanol:methyl-tertiary-butyl-ether (MTBE) (90:10, v/v) at a flow rate of 0.9 ml/min as mobile phase.…”

Section: Methods 1: Hplc Analysis Of Carotenoidsmentioning

confidence: 99%

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Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins

Thomas

Harrison

2016

Journal of Lipid Research

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Section: Carotenoids Are Not Extensively Metabolized By Arpe-19 Cellssupporting

confidence: 70%

Section: Methods 1: Hplc Analysis Of Carotenoidsmentioning

confidence: 99%

Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins

Thomas

Harrison

2016

Journal of Lipid Research

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“…The H-NMR result in Fig. 5 showed the similar peak pattern to those of Aman's work [14] and Khachik's work [15]. From the result, it can be concluded that the purified free lutein sample contained only free lutein, free lutein's stereoisomers and anhydrolutein.…”

Section: Liquid Chromatography Purificationsupporting

confidence: 69%

Purification of Free Lutein from Marigold Flowers by Liquid Chromatography

Boonnoun¹,

Opaskonkun²,

Prasitchoke³

et al. 2012

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Abstract.Marigold is a rich source of a Xanthophyll called lutein, which shows antioxidant and anti-cancer activities and is beneficial to eye health. This has led to various extraction and purification studies to obtain the high purity free lutein suitable for human applications. Liquid chromatography is extensively used to purify high value natural compounds because the process results in high purity products. The suitable mobile phase and stationary phase are the key factors to achieve high purity. In this work, chromatographic separation of lutein extracted from marigold flowers was investigated, using silica gel and mixture of hexane:ethyl acetate as a stationary and a mobile phase, respectively. Initially, the suitable composition of hexane:ethyl acetate was determined using a thin layer chromatography. Hexane:ethyl acetate mixture at 70:30 volume ratio was found to be an appropriate mobile phase for a normal phase chromatographic separation of free lutein. Preliminary experiments on a semi-preparative and a preparative column carried out at the mobile phase flow rate of 10 ml/min suggested that as high as 97.1 % purity free lutien could be obtained with a 60 % approximate yield. Moreover, since the separation by chromatography arises from the adsorption of free lutein onto silica gel, thus to better understand the process, a batch adsorption study was carried out to obtain the equilibrium adsorption data. The isotherm plotted from these data was found to be reasonably described by Langmuir adsorption model.

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“…A quinta fração de EB-DCM, a amostra FE DCM DCM 1, apresentou, no gradiente de polaridade 4,25 (Tabela 2), duas manchas azuis e uma amarela, indicando ser este um gradiente de polaridade que permite separar os carotenoides da clorofila, em uma mesma eluição. Em relação à amostra FE DCM DCM 2, analisada por CP (Tabela 2), apenas foram observadas cor amarelo-claro (AMC) no papel, nos 10 (43,44).…”

Section: Resultsunclassified

ESTUDO FITOQUÍMICO DE Hydrangea sp. POR MEIO DE MÉTODOS CLÁSSICOS DE ANÁLISE POR ESPECTROSCOPIA NO ULTRAVIOLETA VISÍVEL (UV-VIS) E CROMATOGRAFIA EM COLUNA E EM PAPEL

Bernardi

Nicolini

2017

Infarma

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The phytochemical research is an issue that can be approach by several areas of science. The Hydrangea sp., also known in Brazil, as "hortênsia" is an ornamental plant widely known because the presence of flavonoids, a class of antioxidant compounds, which can interact with aluminum ions in the soil, changing the color of the sepals of the Hydrangea flowers. In this work, the objective was to extract chlorophyll from leaves of Hydrangea sp. using three different solvents (dichloromethane, acetone and aqueous solution of acetone, 80 %). The obtained results indicated that in addition to chlorophyll, there were strong spectroscopic evidence of the presence of the carotenoids β-carotene and/or lutein, and lycopene. The used method can be applied to explain that: i) the leaves are green by the presence of the pigment chlorophyll; however, ii) they not only contain chlorophyll in their constitution, also have yellow and reddish pigments and; iii) the aging leaves lose their green color, by the breakage of the resonance of the porphyrin ring from chlorophyll and hence the other colors in the plant predominates. Keywords: carotenoids; chlorophyll; Hydrangea RESUMOA investigação fitoquímica é um tema que pode ser abordado por diversas áreas da ciência. A Hydrangea sp., também conhecida com hortensia, é um planta ornamental muito conhecida por possuir flavonoides, compostos antioxidantes, que interagem com íons alumínio no solo, alterando as cores das sépalas das flores. Este trabalho teve como objetivo extrair clorofila das folhas de Hydrangea sp. utilizando três solventes (diclorometano, acetona e solução aquosa de acetona, 80 %). Os dados indicaram que além de extrair clorofila, também há fortes evidências espectroscópicas da presença dos carotenoides β-caroteno e/ou luteína e licopeno. Assim, o método utilizado pode ser aplicado em aulas práticas para explicar que: i) As folhas são verdes pela presença do pigmento clorofila; ii) no entanto, elas não contém apenas clorofila em sua constituição; também possuem pigmentos amarelos e avermelhados; e iii) ao envelhecer, as folhas perdem a coloração verde, há pela quebra da ressonância do anel porfirínico da clorofila e, consequentemente, as outras cores presentes no vegetal tornam-se predominantes.

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Application of HPLC coupled with DAD, APcI-MS and NMR to the analysis of lutein and zeaxanthin stereoisomers in thermally processed vegetables

Cited by 118 publications

References 34 publications

Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins

Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins

Purification of Free Lutein from Marigold Flowers by Liquid Chromatography

ESTUDO FITOQUÍMICO DE Hydrangea sp. POR MEIO DE MÉTODOS CLÁSSICOS DE ANÁLISE POR ESPECTROSCOPIA NO ULTRAVIOLETA VISÍVEL (UV-VIS) E CROMATOGRAFIA EM COLUNA E EM PAPEL

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