Application of High Performance Liquid Chromatography/Mass Spectrometry with Thermospray Ionization to the Detection of Quassinoids Extracted fromQuassia amara L.

Development and Validation of Liquid Chromatography Combined with Tandem Mass Spectrometry Methods for the Quantitation of Simalikalactone E in Extracts of Quassia amara L. and in Mouse Blood

“…Vitányi et al . () described a reversed‐phase high‐performance (HP) LC–MS method with thermospray ionisation after post‐column addition of ammonium acetate to quantitate the [M + H] + and [M + NH 4 ] + ions of three quassinoids (quassin, neoquassin and 18‐hydroxyquassin). More recently, Pohlit et al .…”

“…Quassinoids have been analysed using liquid chromatography (LC) coupled to mass spectrometry (MS; for a review, see Chakraborty and Pal, 2013). Vitányi et al (1997) described a reversed-phase high-performance (HP) LC-MS method with thermospray ionisation after post-column addition of ammonium acetate to quantitate the [M + H] + and [M + NH 4 ] + ions of three quassinoids (quassin, neoquassin and 18-hydroxyquassin). More recently, Pohlit et al (2009) quantitated the quassinoids isobrucein B and neosergeolide in Picrolemma sprucei infusions using triple quadrupole HPLC with electrospray ionisation (ESI) MS/MS and multiple reaction monitoring (MRM), with limits of quantitation (LOQ) of 0.25 and 0.5 mg/L respectively.…”