Developing a validated liquid chromatography–mass spectrometric method for the simultaneous analysis of five bioactive quassinoid markers for the standardization of manufactured batches of Eurycoma longifolia Jack extract as antimalarial medicaments

Anti-angiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function

“…1. Results from this study are consistent with previous reports suggesting that eurycomanone is one of the major chemical constituents of TAF273 along with other quassinoids (Darise et al, 1982;Teh et al, 2011).…”

“…These findings may suggest that the anti-angiogenic activity may be partly attributed to the quassinoids, which are abundant in TAF273 (Teh et al, 2011).…”

“…Similarly all reference compounds (5 mg) were dissolved in 5 ml of methanol and then filtered. Contents of eurycomanone, 13α(21)-epoxyeurycomanone, 13,21-dihydroeurycomanone and eurycomanol were determined by HPLC fingerprint analysis (Teh et al, 2011).…”

Review on Eurycoma longifolia Pharmacological and Phytochemical Properties

“…However, these methods were not sensitive to detect non-chromophoric constituents; eurycomanol and eurycomanol-2-O-β-D-glucopyranoside present in E. longifolia. Teh et al (2011) developed and validated a HPLC method using electrospray ionization (ESI) and atmospheric pressure chemical (APCI) in positive and negative ion modes for the simultaneous determination of five quassinoid markers; eurycomanone, 13α(21)-epoxyeurycomanone, eurycomanol, eurycomanol-2-O-β-dglucopyranoside and 13,21-dihydroeurycomanone for standardization of manufactured batches of E. longifolia extracts as anti-malarial medicaments. The results showed these product batches varied in range of each constituents concentration probably due to the age of harvesting and growing conditions.…”

Kinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of <i>Eurycoma longifolia</i> Jack Aqueous Extract

“…Eurycoma longifolia Jack contains quassinoids such as 14, 15-dihydroxy-klaineanone, eurycomanone, 13, 21-dihydro-eurycomanone, 13α (21)-epoxy-eurycomanone, 14,15 βdihydroxy-klaineanone, longilactone and eurycomalactone (Han et al, 2015), eurycomanol, eurycomanol-2-O-β-D-glucopyranoside (Teh et al, 2011), alkaloids, glycosides (Bhat and Karim, 2010), glycoproteins (Sambandan et al, 2006), biophenylneolignans, triterpenes, canthine-6-one and β-carboline (Guo et al, 2005).…”