Quassinoids: Chemistry and Novel Detection Techniques

Chakraborty, Debraj; Pal, Amita

doi:10.1007/978-3-642-22144-6_144

Cited by 9 publications

(11 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a first approach, we decided to analyze the contents of the active compounds in the lyophilized extracts used in the present study. The presence of ecdysteroids, such as turkesterone and β-sitosterol, as well as eurycomanone and other quassinoids in herbal extracts, have been analyzed by using methanol for sample extraction, as it was used in the present study [73][74][75][76]. The initial screening was performed spectrophotometrically, and the identification of active compounds was carried out by LC-MS, and quantified by LC-UV [77], using HDL-cholesterol as standard [78] , E.…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Exercise and Phytoanabolic Extracts in Castrated Male and Female Mice

et al. 2021

View full text Add to dashboard Cite

Dry extracts from the Eurasian plants, Ajuga turkestanica, Eurycoma longifolia, and Urtica dioica have been used as anabolic supplements, despite the limited scientific data on these effects. To assess their actions on early sarcopenia signs, male and female castrated mice were supplemented with lyophilized extracts of the three plants, isolated or in association (named TLU), and submitted to resistance exercise. Ovariectomy (OVX) led to body weight increase and non-high-density cholesterol (HDL) cholesterol elevation, which had been restored by exercise plus U. dioica extract, or by exercise and TLU, respectively. Orchiectomy (ORX) caused skeletal muscle weight loss, accompanied by increased adiposity, being the latter parameter reduced by exercise plus E. longifolia or U. dioica extracts. General physical activity was improved by exercise plus herbal extracts in either OVX or ORX animals. Exercise combined with TLU improved resistance to fatigue in OVX animals, though A. turkestanica enhanced the grip strength in ORX mice. E. longifolia or TLU also reduced the ladder climbing time in ORX mice. Resistance exercise plus herbal extracts partly altered gastrocnemius fiber size frequencies in OVX or ORX mice. We provide novel data that tested ergogenic extracts, when combined with resistance exercise, improved early sarcopenia alterations in castrated male and female mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined Effects of Exercise and Phytoanabolic Extracts in Castrated Male and Female Mice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Anticancer activity of eurycomanonehas been contributed by the α,β-unsaturated ketone group present in the eurycomanone structure. The α,β-unsaturated ketone group also play a crucial role in anti-inflammatory activity through the inhibition of NF-κB pathway [8,[37][38][39][40].…”

Section: Ic 50 Of Eurycomanonementioning

confidence: 99%

Cytotoxicity of Eurycomanone and Fargesin in RAW 264.7 Murine Macrophages

Rosly

Roof

Ramli

et al. 2021

JPRI

View full text Add to dashboard Cite

Bioactive natural compounds derived from plants are the source for the development of new drugs. Numerous in vitro studies have explored the anti-inflammatory effect of eurycomanone and fargesin, derived from Eurycoma longifolia and Flosmagnoliae, respectively. However, before anti-inflammatory investigation is conducted, it is important to obtain the safe doses of these compounds to ensure the validity of the anti-inflammatory results. Therefore, the present study was aimed to investigate the cytotoxicity of eurycomanone and fargesin towards macrophage RAW 264.7. cells to determine the safe doses of these compounds. Different concentrations of eurycomanone and fargesin were subjected to RAW 264.7 cells. The cytotoxicity of the compounds was evaluated by MTT assay and 50% inhibitory concentration (IC50) of these compounds was determined. Morphological changes of RAW 264.7 cells upon exposure to these compounds were also observed. Eurycomanone exhibited its cytotoxic effect by reducing RAW 264.7 cell viability dose-dependently with the IC50 of 94.17 µM. Meanwhile, fargesin had slight cytotoxicity towards RAW 264.7 cells with the IC50 of 173.5µM. Eurycomanone was more cytotoxic towards RAW 264.7 cells compared to fargesin. In conclusion, eurycomanone and fargesin at concentration up to 25 µM, was not toxic to the RAW 264.7 murine macrophages cells and the findings can be applied in the future anti-inflammatory study.

show abstract

“…Quassinoids have been analysed using liquid chromatography (LC) coupled to mass spectrometry (MS; for a review, see Chakraborty and Pal, ). Vitányi et al .…”

Section: Introductionmentioning

confidence: 97%

“…Quassinoids have been analysed using liquid chromatography (LC) coupled to mass spectrometry (MS; for a review, see Chakraborty and Pal, 2013). Vitányi et al (1997) described a reversed-phase high-performance (HP) LC-MS method with thermospray ionisation after post-column addition of ammonium acetate to quantitate the [M + H] + and [M + NH 4 ] + ions of three quassinoids (quassin, neoquassin and 18-hydroxyquassin).…”

Section: Introductionmentioning

confidence: 99%