Application of Extracellular Vesicles in Aquatic Animals: A Review of the Latest Decade

Zhao, Na; Deng, Qiuxia; Zhu, Chunhua; Zhang, Bo

doi:10.1080/23308249.2021.1985429

Cited by 4 publications

(11 citation statements)

References 179 publications

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“…CircRNAs are novel ncRNAs with a stable structure of covalently closed continuous loops ( 173 ). Current data show that circRNAs can act as miRNA sponges and subsequently inactivate the post-transcriptional attenuation functions of corresponding miRNAs ( 174 , 175 ). To date, most studies concerning circRNAs have focused on human diseases; studies on circRNAs’ targets (such as mRNA and miRNA) are largely lacking.…”

Section: Cernas Involved In the Nf-κb Pathwaysmentioning

confidence: 99%

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

Zhao

Zou²,

Yan³

et al. 2023

Front. Immunol.

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Nuclear factor-kappa B (NF-κB) pathways have a close relationship with many diseases, especially in terms of the regulation of inflammation and the immune response. Non-coding RNAs (ncRNAs) are a heterogeneous subset of endogenous RNAs that directly affect cellular function in the absence of proteins or peptide products; these include microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), etc. Studies on the roles of ncRNAs in targeting the NF-κB pathways in aquatic animals are scarce. A few research studies have confirmed detailed regulatory mechanisms among ncRNAs and the NF-κB pathways in aquatic animals. This comprehensive review is presented concerning ncRNAs targeting the NF-κB pathway in aquatic animals and provides new insights into NF-κB pathways regulatory mechanisms of aquatic animals. The review discusses new possibilities for developing non-coding-RNA-based antiviral applications in fisheries.

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Section: Cernas Involved In the Nf-κb Pathwaysmentioning

confidence: 99%

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

Zhao

Zou²,

Yan³

et al. 2023

Front. Immunol.

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“…Exosomes are 30–150 nm extracellular vesicles with a lipid bilayer structure and contain various nucleic acids, proteins, and lipids [ 20 , 21 , 22 ]. Exosomes are transported from donor cells to recipient cells and considered important regulatory molecules involved in various functions, including intercellular communication and transport, providing potential biomarkers for diagnosis and monitoring [ 20 , 21 , 22 , 23 , 24 , 25 ]. Piwi-interacting RNAs (piRNAs) are a class of 26–31 nt small RNAs.…”

Section: Introductionmentioning

confidence: 99%

“…Piwi-interacting RNAs (piRNAs) are a class of 26–31 nt small RNAs. They were initially discovered in mammalian germ cells in 2006 [ 26 , 27 ] and are important components of exosomes in teleosts [ 20 ]. They have roles in transposon silencing, gametogenesis, the maintenance of genomic integrity, and stem cell lineages [ 20 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…They were initially discovered in mammalian germ cells in 2006 [ 26 , 27 ] and are important components of exosomes in teleosts [ 20 ]. They have roles in transposon silencing, gametogenesis, the maintenance of genomic integrity, and stem cell lineages [ 20 , 28 , 29 , 30 ]. Sex-inclined piRNAs are involved in various physiological processes, including sex development, sex differentiation, sex determination, and sex regulation in aquatic animals [ 20 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…They have roles in transposon silencing, gametogenesis, the maintenance of genomic integrity, and stem cell lineages [ 20 , 28 , 29 , 30 ]. Sex-inclined piRNAs are involved in various physiological processes, including sex development, sex differentiation, sex determination, and sex regulation in aquatic animals [ 20 , 30 , 31 , 32 , 33 , 34 ]. We have previously shown that sex-inclined exosomal piRNAs could be employed as biomarkers to identify the male and pseudo-male fish, with the same karyotype as female fish and the same physiological characteristics as male fish [ 34 ], in half smooth tongue sole ( Cynoglossus semilaevis ) [ 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Sex-Inclined Piwi-Interacting RNAs in Serum Exosomes for Sex Determination in the Greater Amberjack (Seriola dumerili)

Deng

Zhao

et al. 2023

IJMS

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The greater amberjack (Seriola dumerili) is a gonochoristic fish with no sexual dimorphism in appearance, making sex identification difficult. Piwi-interacting RNAs (piRNAs) function in transposon silencing and gametogenesis and are involved in various physiological processes, including sex development and differentiation. Exosomal piRNAs can be indicators for the determination of sex and physiological status. In this study, four piRNAs were differentially expressed in both serum exosomes and gonads between male and female greater amberjack. Three piRNAs (piR-dre-32793, piR-dre-5797, and piR-dre-73318) were significantly up-regulated and piR-dre-332 was significantly down-regulated in serum exosomes and gonads of male fish, compared to female fish, consistent with the serum exosomal results. According to the relative expression of four marker piRNAs derived from the serum exosomes of greater amberjack, the highest relative expression of piR-dre-32793, piR-dre-5797, and piR-dre-73318 in seven female fish and that of piR-dre-332 in seven male fish can be used as the standard for sex determination. The method of sex identification can ascertain the sex of greater amberjack by blood collection from the living body, without sacrificing fish. The four piRNAs did not show sex-inclined expression in the hypothalamus, pituitary, heart, liver, intestine, and muscle tissue. A piRNA–target interaction network involving 32 piRNA-mRNA pairs was generated. Sex-related target genes were enriched in sex-related pathways, including oocyte meiosis, transforming growth factor-beta signaling pathway, progesterone-mediated oocyte maturation, and gonadotropin releasing hormone signaling pathway. These results provide a basis for sex determination in greater amberjack and improve our understanding of the mechanisms underlying sex development and differentiation in the species.

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Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

Pelyhe,

Sturve

2023

Fish Physiol Biochem

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Extracellular vesicles (EVs) are 50–1,000 nm lipid bilayer-bound vesicles, released into the extracellular environment by various cell types for intercellular communication purposes. The quantitative and qualitative characteristics of EVs can be affected by stress and pathological conditions. The majority of extracellular vesicle (EV) studies have been performed on mammalian cell lines or bodily fluids. EVs have been previously described from bodily fluids like plasma, serum or mucus in different fish species, however the available knowledge of fish cell line derived EVs is limited and in the vast majority of studies, the overall focus is on small EVs (< 200 nm). We isolated large and small extracellular vesicles from zebrafish (Danio rerio) liver (ZFL), rainbow trout (Oncorhynchus mykiss) liver (RTL-W1), gill (RTgill-W1) and intestinal epithelial (RTgutGC) cell lines using stepwise centrifugation and characterized the size and morphology of EVs. Here we demonstrated that large and small extracellular vesicles can be successfully isolated using stepwise centrifugation from the serum-free medium of the selected piscine cell lines after a 24-h incubation period. The size distribution of large and small EVs isolated from the piscine cell lines suggest that large and small EV groups show high diversity in size ranges, containing heterogenous subpopulations in sizes, and the results highly depend on the applied method and whether filtration steps were included following the isolation. The spherical morphology of EVs was verified by transmission electron microscopy.

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Application of Extracellular Vesicles in Aquatic Animals: A Review of the Latest Decade

Cited by 4 publications

References 179 publications

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

Non-coding RNAs targeting NF-κB pathways in aquatic animals: A review

Sex-Inclined Piwi-Interacting RNAs in Serum Exosomes for Sex Determination in the Greater Amberjack (Seriola dumerili)

Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

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