MicroRNA-7 (miR-7) is a highly conserved short non-coding RNA involved in various bioprocesses via the regulation of multiple target genes. To enrich our knowledge of the functions of miR-7 in innate immune regulation in echinoderms, we first investigated the targeting relationship between miR-7 and PAK1 in the sea cucumber Apostichopus japonicus and then explored the functions of miR-7, the PAK1 gene, and the miR-7/PAK1 axis in the pathogen-induced immune response of A. japonicus. Our results showed that miR-7 can bind to the 3ʹUTR of PAK1 and negatively regulate the expression of PAK1 in A. japonicus. Overexpression and inhibition of miR-7 and inhibition of the expression of PAK1 can alter phagocytosis, cellular agglutination, and lysozyme contents in A. japonicus. Both miR-7 and the PAK1 gene are involved in immune defense against Vibrio splendidus infection; the miR-7/AjPAK1 axis showed immune regulatory function at 48 to 72 h post-infection (hpi) after V. splendidus infection in A. japonicus. In summary, the results of this study established that miR-7 regulates the pathogen-induced immune response by targeting PAK1 in A. japonicus.
Appreciation education is an educational method that helps students to get better development by finding and encouraging their shining points. With the deepening reform of quality education, the society's concept of education has changed. It not only requires the all-round development of students' moral grounding, intellectual ability, physical vigor, aesthetic sensibility, and work skills, but also encourages the application of appreciation for students' education and classroom management. How to understand and use appreciation education has become the focus. By digging deeper and analyzing the connotation, theoretical basis and role of appreciation education, this study summarizes the current situation, characteristics and citation of the application of appreciation education in order to enhance the effectiveness of mathematics teaching and provide guidelines for theoretical researchers and front-line practitioners.
The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.
Nuclear factor-kappa B (NF-κB) pathways have a close relationship with many diseases, especially in terms of the regulation of inflammation and the immune response. Non-coding RNAs (ncRNAs) are a heterogeneous subset of endogenous RNAs that directly affect cellular function in the absence of proteins or peptide products; these include microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), etc. Studies on the roles of ncRNAs in targeting the NF-κB pathways in aquatic animals are scarce. A few research studies have confirmed detailed regulatory mechanisms among ncRNAs and the NF-κB pathways in aquatic animals. This comprehensive review is presented concerning ncRNAs targeting the NF-κB pathway in aquatic animals and provides new insights into NF-κB pathways regulatory mechanisms of aquatic animals. The review discusses new possibilities for developing non-coding-RNA-based antiviral applications in fisheries.
An image encryption algorithm for the double scrambling of the pixel position and bit was cryptanalyzed. In the original image encryption algorithm, the positions of pixels were shuffled totally with the chaotic sequence. Then, the 0 and 1-bit positions of image pixels were scrambled through the use of another chaotic sequence generated by the input key. The authors claimed that the algorithm was able to resist the chosen-plaintext attack. However, through the analysis of the encryption algorithm, it was found that the equivalent key of the whole encryption algorithm was the scrambling sequence T in the global scrambling stage, the pixel bit level scrambling sequence WT and the diffusion sequence S. The generation of scrambling sequence T is related to the sum of all pixel values of the plaintext image, while the generation of WT and S is not associated with the image to be encrypted. By using a chosen-plaintext attack, these equivalent key streams can be cracked so as to realize the decoding of the original chaotic encryption algorithm. Both theoretical analysis and experimental results verify the feasibility of the chosen-plaintext attack strategy. Finally, an improved algorithm was proposed to overcome the defect, which can resist the chosen-plaintext attack and has the encryption effect of a “one time pad”.
The operation mode of distribution network is diversified, and it is difficult to accurately locate the fault point when the fault occurs in the case of multiple power sources and branches. In order to accurately locate the fault point and quickly restore the power supply in the fault-free area, a bisection fault location method based on improved matrix is proposed. According to the feedback information of terminal feeder equipment, the improved matrix algorithm is used to form the grid structure matrix and fault information matrix base on the connection relationship of distribution network. The grid structure matrix reflects the connection relationship between nodes and the power flow direction of the distribution network in normal operation. The fault information matrix is the flow direction of power flowing through terminal equipment when the system fails. According to the grid matrix and fault information matrix, the fault judgment matrix is obtained to judge the fault area. Finally, the dichotomy method is used to judge the fault area and determine the specific fault point. The effectiveness and practicability of the method are verified by an example.
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