Application of dedifferentiated fat cells for periodontal tissue regeneration

Sugawara, Atsunori; Sato, Soh

doi:10.1007/s13577-013-0075-6

Cited by 26 publications

(21 citation statements)

References 16 publications

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“…Newly formed alveolar bone was observed by HE staining after 4 weeks of cell transplantation. Small amounts of newly formed cementum‐like structures were also observed on the exposed root surface (Sugawara & Sato, ). Akita et al.…”

Section: Dfat Cells For Oral and Maxillofacial Tissue Engineering—in mentioning

confidence: 99%

“…Newly formed alveolar bone was observed by HE staining after 4 weeks of cell transplantation. Small amounts of newly formed cementum-like structures were also observed on the exposed root surface (Sugawara & Sato, 2014 T A B L E 1 Expression pattern of CD antigens in human Dedifferentiated Fat (DFAT) cells and in other types of stem cells alveolar bone than the ASCs+PLGA or PLGA alone groups . Kikuta and colleagues seeded DFAT cells in β-tricalcium phosphate (β-TCP)/collagen I sponges and cultured in OIM for 2 weeks to form tissue-engineered bone (TEB).…”

Section: Periodontal Tissue Regenerationmentioning

confidence: 99%

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Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

et al. 2018

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Tissue engineering is a promising method for the regeneration of oral and maxillofacial tissues. Proper selection of a cell source is important for the desired application. This review describes the discovery and usefulness of dedifferentiated fat (DFAT) cells as a cell source for tissue engineering. Dedifferentiated Fat cells are a highly homogeneous cell population (high purity), highly proliferative, and possess a multilineage potential for differentiation into various cell types under proper in vitro inducing conditions and in vivo. Moreover, DFAT cells have a higher differentiation capability of becoming osteoblasts, chondrocytes, and adipocytes than do bone marrow-derived mesenchymal stem cells and/or adipose tissue-derived stem cells. The usefulness of DFAT cells in vivo for periodontal tissue, bone, peripheral nerve, muscle, cartilage, and fat tissue regeneration was reported. Dedifferentiated Fat cells obtained from the human buccal fat pad (BFP) are a minimally invasive procedure with limited esthetic complications for patients. The BFP is a convenient and accessible anatomical site to harvest DFAT cells for dentists and oral surgeons, and thus is a promising cell source for oral and maxillofacial tissue engineering.

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Section: Dfat Cells For Oral and Maxillofacial Tissue Engineering—in mentioning

confidence: 99%

Section: Periodontal Tissue Regenerationmentioning

confidence: 99%

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

et al. 2018

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“…According to a systematic review, PDL-derived cells showed a favorable effect on new PDL formation, although there was no statistically significant difference between PDL-derived or Bone marrow derived stem cells [48]. Other cell types like DPSC [49], GMSC [50], DFAT [51], SHED [52] have also been able to successfully regenerate periodontal tissue.…”

Section: Periodontal Complexmentioning

confidence: 99%

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Kanwal¹,

D’souza²,

Muthiah³

et al. 2017

OJST

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Aim: 1) To review the literature of various applications of stem cell regeneration in dentistry from 2010 to 2016. 2) To review these studies and to summarize the current status of stem cell regeneration in intra oral applications.3) To present the available successful data with regard to stem cell regeneration in dentistry and to highlight the future trends. Materials and Methods: Search Protocol: A systematic search was made in the PubMed database with the key words gingiva, papillary reconstruction, periodontal ligament, dental pulp, salivary gland, enamel re-mineralization, dentin, cementum, bone, whole tooth, cleft palate, regeneration and stem cells. All articles from 2010 to 2016, relevant to the topic were included. After the search a total of 1826 articles were obtained which were screened and categorized by three independent reviewers as review papers, in vitro, animal and human studies, pertaining to stem cell regeneration in intra oral applications. On the basis of the extracted data and outcome measures, conclusions were drawn. Results: Maximum number of animal studies has been done to regenerate periodontal tissues, bone, dentin and pulp tissues. Few human studies have been done till date. Although clinically, researchers have been able to regenerate periodontal-like tissue, bone and pulp-like tissue, they still haven't been able to regenerate tissues that completely resemble tissues in their natural form. Conclusion: The future of stem cell therapy in dental applications looks promising. The predictability and efficacy of outcomes, as well as safety concerns of stem cell therapy is yet to be fully established. Ongoing research and development of newer scaffolds, understanding various signaling molecules and their cues, understanding gene expression and proteomics of stem cells are the future directions that will take us a step forward to achieving successful regeneration.

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“…DFAT cells have emerged as a potential cell source for regenerative medicine because of their transdifferentiation capability and similarity to ASCs and bone marrow MSCs [75][76][77] . Multiple studies have demonstrated differentiation of DFAT cells into multiple lineages including adipogenic, osteogenic, chondrogenic, myogenic, angiogenic and neurogenic lineages (Figure 3).…”

Section: Differentiation Potential Of Dfat Cellsmentioning

confidence: 99%

Dedifferentiated fat cells: A cell source for regenerative medicine

Jumabay¹,

Boström²

2015

WJSC

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The identification of an ideal cell source for tissue regeneration remains a challenge in the stem cell field. The ability of progeny cells to differentiate into other cell types is important for the processes of tissue reconstruction and tissue engineering and has clinical, biochemical or molecular implications. The adaptation of stem cells from adipose tissue for use in regenerative medicine has created a new role for adipocytes. Mature adipocytes can easily be isolated from adipose cell suspensions and allowed to dedifferentiate into lipidfree multipotent cells, referred to as dedifferentiated fat (DFAT) cells. Compared to other adult stem cells, the DFAT cells have unique advantages in their abundance, ease of isolation and homogeneity. Under proper condition in vitro and in vivo , the DFAT cells have exhibited adipogenic, osteogenic, chondrogenic, cardiomyogenc, angiogenic, myogenic, and neurogenic potentials. In this review, we first discuss the phenomena of dedifferentiation and transdifferentiation of cells, and then dedifferentiation of adipocytes in particular. Understanding the dedifferentiation process itself may contribute to our knowledge of normal growth processes, as well as mechanisms of disease. Second, we highlight new developments in DFAT cell culture and summarize the current understanding of DFAT cell properties. The unique features of DFAT cells are promising for clinical applications such as tissue regeneration.

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Application of dedifferentiated fat cells for periodontal tissue regeneration

Cited by 26 publications

References 16 publications

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Dedifferentiated fat cells: A cell source for regenerative medicine

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