NMR
metabolomics are primarily conducted with 1D nuclear Overhauser
enhancement spectroscopy (NOESY) presat for water suppression and
Carr–Purcell–Meiboom–Gill (CPMG) presat as a
T2 filter to remove macromolecule signals. Others pulse
sequences exist for these two objectives but are not often used in
metabolomics studies, because they are less robust or unknown to the
NMR metabolomics community. However, recent improvements on alternative
pulse sequences provide attractive alternatives to 1D NOESY presat
and CPMG presat. We focus this perspective on PURGE, a water suppression
technique, and PROJECT presat, a T2 filter. These two pulse
sequences, when optimized, performed at least on par with 1D NOESY
presat and CPMG presat, if not better. These pulse sequences were
tested on common samples for metabolomics, human plasma, and urine.