Application of comprehensive NMR‐based analysis strategy in annotation, isolation and structure elucidation of low molecular weight metabolites of <i>Ricinus communis</i> seeds

Vučković, Ivan; Rapinoja, Marja-Leena; Vaismaa, Matti J. P.; Vanninen, Paula; Koskela, Harri

doi:10.1002/pca.2600

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…Since its first publication, the PURGE pulse sequence has been used on a regular basis by the team that created it, 23 − 35 along with several other groups, 36 − 46 and even suggested in a Nature protocol. 47 Despite this visibility, the PURGE pulse sequence has not become routine in metabolomics.…”

Section: Alternate Water Suppression Techniques and Avoiding Some Of mentioning

confidence: 99%

Alternatives to Nuclear Overhauser Enhancement Spectroscopy Presat and Carr–Purcell–Meiboom–Gill Presat for NMR-Based Metabolomics

2017

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NMR metabolomics are primarily conducted with 1D nuclear Overhauser enhancement spectroscopy (NOESY) presat for water suppression and Carr–Purcell–Meiboom–Gill (CPMG) presat as a T2 filter to remove macromolecule signals. Others pulse sequences exist for these two objectives but are not often used in metabolomics studies, because they are less robust or unknown to the NMR metabolomics community. However, recent improvements on alternative pulse sequences provide attractive alternatives to 1D NOESY presat and CPMG presat. We focus this perspective on PURGE, a water suppression technique, and PROJECT presat, a T2 filter. These two pulse sequences, when optimized, performed at least on par with 1D NOESY presat and CPMG presat, if not better. These pulse sequences were tested on common samples for metabolomics, human plasma, and urine.

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Section: Alternate Water Suppression Techniques and Avoiding Some Of mentioning

confidence: 99%

Alternatives to Nuclear Overhauser Enhancement Spectroscopy Presat and Carr–Purcell–Meiboom–Gill Presat for NMR-Based Metabolomics

2017

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“…The principle that the presence of non-toxic metabolites indicates the presence of toxic ones has been also demonstrated in the literature for detection of ricin. Related low mass metabolites from Ricinus communis seeds indicated the presence of highly toxic protein ricin [44].…”

Section: Relationships Between Biomarkers and Toxic Principles In The...mentioning

confidence: 99%

Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum

et al. 2022

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Allium ursinum and poisonous adulterants Convallaria majalis and Arum maculatum were used as a model for detection of adulterants in edible plant. A. ursinum samples were spiked with C. majalis and A. maculatum to mimic adulteration. Metabolomic fingerprinting of all samples was performed using 1H NMR spectroscopy, and the resulting data sets were subjected to multivariate data analysis. As a result of this analysis, signals of adulterants were extracted from the data, and the structures of biomarkers of adulteration from partially purified samples were elucidated using 2D NMR and LC-MS techniques. Thus, isovitexin and vicenin II, azetidine-2-carboxylic acid, and trigonelline indicated adulteration of A. ursinum samples with C. majalis. Isovitexin was also recognized to be an indicator of adulteration of A. ursinum with A. maculatum. In conclusion, the case study of A. ursinum suggested that plant metabolomics approach could be utilized for identification of low molecular weight biomarkers of adulteration in edible plants.

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“…However, the low sensitivity of NMR spectroscopy hindered our efforts to detect minor reaction intermediates. Therefore, sensitivity enhancement techniques such as dynamic nuclear polarization (DNP) NMR methods and/or presaturation methods to increase the dynamic range of the proton spectra by pre‐saturating large water signals, are expected to be useful to detect low‐concentration species. In the future, the amount of methanol crossover will be calibrated by simultaneously analyzing anode and cathode exhausts, and the evaporation of methanol will be calibrated by measuring the time‐dependent concentration of methanol at 80°C.…”

Section: Nmr Peak Areas and Widths Of Anode Exhausts And Currents Of mentioning

confidence: 99%

Nuclear Magnetic Resonance Spectroscopic Investigation of Anode Exhaust of Direct Methanol Fuel Cells Without Isotope Enrichment

Byun

Hwang

Han

2016

Bulletin Korean Chem Soc

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Application of comprehensive NMR‐based analysis strategy in annotation, isolation and structure elucidation of low molecular weight metabolites of Ricinus communis seeds

Abstract: The applied comprehensive NMR-based analysis strategy provided identification of the prominent low molecular weight metabolites with high confidence.

Cited by 5 publications

References 30 publications

Alternatives to Nuclear Overhauser Enhancement Spectroscopy Presat and Carr–Purcell–Meiboom–Gill Presat for NMR-Based Metabolomics

Alternatives to Nuclear Overhauser Enhancement Spectroscopy Presat and Carr–Purcell–Meiboom–Gill Presat for NMR-Based Metabolomics

Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum

Nuclear Magnetic Resonance Spectroscopic Investigation of Anode Exhaust of Direct Methanol Fuel Cells Without Isotope Enrichment

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