Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum

Ivanović, Stefan; Simić, Katarina; Lekić, Stefan; Jadranin, Milka; Vujisić, Ljubodrag; Gođevac, Dejan

doi:10.3390/metabo12090849

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…NMR spectroscopy is largely used in the field of quality evaluation of various food and medicinal plants, and it is widely used for the characterization and structure determination of natural products. NMR spectrum can provide a wealth of accurate qualitative and quantitative information even regarding the components of a mixture [3]. In most cases, in contrast to GC-MS and LC-UV/MS techniques, the NMR approach does not require separation or isolation of analytes from a mixture.…”

Section: Introductionmentioning

confidence: 99%

LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

Kılınc,

D’Urso,

Paolillo

et al. 2023

Metabolites

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The application of metabolomics to the study of plants is growing because of the current development of analytical techniques. The most commonly used analytical technology driving plant metabolomics studies is Mass Spectrometry (MS) coupled to liquid chromatography (LC). In recent years, Nuclear Magnetic Resonance (NMR) spectroscopy, not requiring a previous chromatographic separation, has been receiving growing attention for metabolite fingerprinting of natural extracts. Herein, an integrated LC-MS and 1H NMR metabolomic approach provided a comprehensive phytochemical characterization of Symphytum anatolicum whole plant, taking into account both primary and specialized metabolites. Moreover, the NMR analyses provided direct quantitative information. Species belonging to the Symphytum genus, known as comfrey, have shown several biological activities including anti-inflammatory, analgesic, hepatoprotective, antifungal, and antibacterial. The LC-MS profile showed the presence of 21 main specialized metabolites, belonging to the classes of flavonoids, phenylpropanoids, salvianols, and oxylipins. The 1H NMR spectrum revealed the occurrence of metabolites including organic acids, phenolics, flavonoids, sugars, and amino acids. A quantitative analysis of these metabolites was performed and their concentration was obtained with respect to the known concentration of TSP, by means of the software package Chenomx which allows quantification of individual components in the NMR spectra. Furthermore, the phenolic content, antioxidant activity, glucosidase, and tyrosinase inhibitory activity of S. anatolicum extract were evaluated. The resulting bioactivity profile suggests how S. anatolicum represents a source of metabolites with health-promoting activity.

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Section: Introductionmentioning

confidence: 99%

LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

Kılınc,

D’Urso,

Paolillo

et al. 2023

Metabolites

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“…As the needle is inserted and then pulled out of the body, a small amount of tissue is sampled onto the needle tip, similar to a biopsy. Considering these features, it was expected that by extending the probe ESI concept to acupuncture needles, a new method that allowed easy sampling and sensitive MS detection could be readily developed and easily implemented, in particular for the rapid in vivo detection of plant metabolites [ 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Surface-Coated Acupuncture Needles as Solid-Phase Microextraction Probes for In Vivo Analysis of Bioactive Molecules in Living Plants by Mass Spectrometry

et al. 2023

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In this work, we report the coupling of solid-phase microextraction (SPME) enabled by surface-coated acupuncture needles with nano-electrospray mass spectrometry (nanoESI-MS) for the analysis of bioactive molecules in living plants. The needle tip was oxidized by a mixture of nitric acid and hydrogen peroxide solution and then subject to surface coating via carbonization of paraffin. A combination of oxidation and surface coating resulted in a thin coating of carbon film, whereby the significantly increased surface area promoted both analyte enrichment and ionization for MS analysis. The analytical performances were evaluated through the characterization of small molecules, peptides and proteins. Compared with conventional nanoESI, our new strategy of employing surface-coated needles had a high salt tolerance. The streamlined experimental workflow could be completed within one minute. The linear dynamic ranges for L-histidine and L-lysine, as two representatives, were over two orders of magnitude with a limit of detection (LOD) of 3.0~5.0 ng/mL. A mark is made on the needle at 2 mm from the tip, the needle is then kept in the sample for 30 s. In vivo sampling and identification of α-tomatine and organic acids from the stem of a living tomato plant were demonstrated as a practical application, while the physiological activities of the plant were not disrupted due to the minimally invasive sampling. We anticipate that the developed strategy may be of potential use for real-time clinical and other on-site analyses.

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Investigation of Cuckoo‐Pint's (Arum maculatum) Phytochemistry, In Vitro Antioxidant Potential, Enzyme Inhibition, and Antimicrobial Activity

Aslan,

Kelle,

Yilmaz

et al. 2024

ChemistrySelect

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Arum maculatum, cuckoo‐pint in folk linguistics, is a poisonous but medicinal plant with tremendous properties that need investigation. This study subjected cuckoo‐pint's parts to in vitro antioxidant, enzyme inhibition, and antimicrobial susceptibility testing to investigate its biological activity. Further, phytochemistry was analyzed by both LC–MS/MS and total phenol, flavonoid, saponin, and reducing sugar content determination assays. Antioxidant activities were determined by Fe3+, Cu2+, Fe3+‐TPTZ metal‐reducing ability, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2ʹ‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) radical scavenging assays. According to the results, seeds and berries have remarkable metal‐reducing and radical‐scavenging effects as natural and synthetic analogs such as ascorbic acid, butylated hydroxyanisole, butylated hydroxytoluene, trolox, and α‐tocopherol. Inhibition studies over human carbonic anhydrase II (hCA II), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α‐amylase were performed, and IC50 values were determined for each enzyme inhibition. According to the results, the cuckoo‐pint seeds inhibited the AChE, BChE, and hCA II activity with greater IC50 values than reference inhibitors. Antimicrobial activity over pan‐resistant strains was achieved for each strain with the extracts, at least 20 mm zone diameter was obtained with the lowest concentrations. LC–MS/MS results revealed 24 bioactive metabolites associated with the extracts’ biological activity. Notably, quinic acid and epicatechin correlated with cardioprotective, neuroprotective, antioxidant, and antidiabetic activity.

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Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum

Cited by 5 publications

References 42 publications

LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

Surface-Coated Acupuncture Needles as Solid-Phase Microextraction Probes for In Vivo Analysis of Bioactive Molecules in Living Plants by Mass Spectrometry

Investigation of Cuckoo‐Pint's (Arum maculatum) Phytochemistry, In Vitro Antioxidant Potential, Enzyme Inhibition, and Antimicrobial Activity

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