Application of chromatography and coupled chromatography-mass spectrometry for the investigation of pharmacokinetics and metabolism of propofol, clofelin, phencylidine, and tramadol (a review)

The present review covers the main research in the area of mass spectrometry from the 1990s which was about the same time as the Russian Federation emerged from the collapse of the Soviet Union (USSR). It consists of two main parts-application of mass spectrometry to chemistry and related fields and creation and development of mass spectrometric technique. Both traditional and comparatively new mass spectrometric methods were used to solve various problems in organic chemistry (reactivity of gas-phase ions, structure elucidation and problems of identification, quantitative and trace analysis, differentiation of stereoisomers, derivatization approaches etc.), biochemistry (proteomics and peptidomics, lipidomics), medical chemistry (mainly the search of biomarkers, pharmacology, doping control), environmental, petrochemistry, polymer chemistry, inorganic and physical chemistry, determination of natural isotope ratio etc. Although a lot of talented mass spectrometrists left Russia and moved abroad after the collapse of the Soviet Union, the vitality of the mass spectral community proved to be rather high, which allowed the continuation of new developments in the field of mass spectrometric instrumentation. They are devoted to improvements in traditional magnetic sector mass spectrometers and the development of new ion source types, to analysis and modification of quadrupole, time-of-flight (ToF) and ion cyclotron resonance (ICR) analyzers. The most important achievements are due to the creation of multi-reflecting ToF mass analyzers. Special attention was paid to the construction of compact mass spectrometers, particularly for space exploration, of combined instruments, such as ion mobility spectrometer/mass spectrometer and accelerating mass spectrometers. The comparatively young Russian Mass Spectrometry Society is working hard to consolidate the mass spectrometrists from Russia and foreign countries, to train young professionals on new appliances and regularly holds conferences on mass spectrometry. For ten years, a special journal Mass-spektrometria has published papers on all disciplines of mass spectrometry.

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“…In other works, GC/MS method hasproved to be very efficient for the study of pharmacokinetics and metabolism of various anestetics. 213,214…”

Section: Pharmacology Drugs and Metabolitesmentioning

confidence: 99%

Mass Spectrometry in Russia

Заикин

Сысоев

2013

Eur J Mass Spectrom (Chichester)

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“…GC/MS, on the other hand, is rugged, selective and cost‐effective in terms of reagent disposal, but it lacks the sensitivity that is possible with RIA. A review by Savchuk et al of coupled chromatographic/mass spectrometric techniques for PCP quantitation in biological samples reported an LLOQ for PCP of 5 ng ml −1 in 200 µl of sample 13. More recently, Ishii et al have used GC with surface ionization organic MS for the quantitation of PCP in whole rat blood 14.…”

Section: Introductionmentioning

confidence: 99%

A validated liquid chromatographic/tandem mass spectrometric method for the determination of phencyclidine in microliter samples of rat serum

2004

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A liquid chromatographic/tandem mass spectrometric method is described for the determination of phencyclidine (PCP) in small volumes of rat serum (e.g. 50 microl). Samples were extracted using a mixed-mode strong cation-exchange column and then separated isocratically using a narrow-bore (2.1 mm i.d.) 3 microm Hypersil phenyl column and a mobile phase consisting of an ammonium formate buffer (pH 2.7) with 60% (v/v) methanol. Detection was accomplished using positive ion electrospray ionization in the multiple reaction monitoring mode. Mass spectra were obtained and peaks were observed at an m/z (% abundance) of 244 (100), 159 (25), and 86 (89). Tandem mass spectra were also obtained from the m/z 244 precursor ion with peaks observed at m/z 159 (100), 86 (96), and 91 (11). Optimum serum PCP sensitivity and precision were obtained at a transition of m/z 244 --> 159. Matrix-associated ion suppression did not significantly affect the accuracy (100-112%) or precision (CV < or =8%) of the assay. The lower limit of quantitation was 1 ng ml(-1) in 50 microl of serum. The method was used to study the serum pharmacokinetics of PCP in rats after an intravenous bolus dose of PCP.

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Analysis of tramadol in pharmaceutical preparations by high performance thin layer chromatography

Meyyanathan¹,

Kumar

Suresh

2003

J of Separation Science

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A new simple, precise, rapid, and selective high-performance thin layer chromatography (HPTLC) method has been developed for the analysis of tramadol in pharmaceutical formulations. The method uses chlorzoxazone as an internal standard. The stationary phase was silica gel 60F 254 prewashed with methanol; ethyl acetate-methanolammonia solution 7 + 1 + 0.5 (v/v) was used as mobile phase. Detection and quantification were performed densitometrically at k = 275 nm. The linear range of the analysis was 1.0 -2.5 lg and the percentage recovery was 104.6%.

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Application of chromatography and coupled chromatography-mass spectrometry for the investigation of pharmacokinetics and metabolism of propofol, clofelin, phencylidine, and tramadol (a review)

Cited by 3 publications

References 71 publications

Mass Spectrometry in Russia

Mass Spectrometry in Russia

A validated liquid chromatographic/tandem mass spectrometric method for the determination of phencyclidine in microliter samples of rat serum

Analysis of tramadol in pharmaceutical preparations by high performance thin layer chromatography

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