Application of Chromatographic and Spectrophotometric Methods for The Analysis of Aliskiren and Hydrochlorothiazide Antihypertensive Combination.

Abstract

High performance liquid chromatographic (HPLC) and spectrophotometric methods developed for the simultaneous determination of Aliskiren (ALK) and Hydrochlorothiazide (HCT) combination in bulk powder and in tablets dosage form. Determination of ALK and HCT was achieved by chromatographic separation on Econosphere C-18 column using a mobile phase consisting of water (pH 7.5): acetonitrile (50:50) at a flow rate of 0.5 mL.min −1 and UV detection at 208 nm. Method validation parameters were found to be acceptable over the concentrations range of 5-150 µg.mL -1 , 1-50 µg.mL -1 for ALK and HCT respectively. Regarding the spectrophotometric methods, two methods were employed. Simultaneous Equation method, absorbance readings are taken at two wavelengths 277.48 nm (for ALK) and 267.48 nm (for HCT) in methanol. Dual Wavelength method, the difference between absorbance readings are taken at two wavelengths 273.3 nm, 260 nm (for Aliskiren) and 270 nm, 280 nm (for HCT) in methanol. The applied spectrophotometric methods were found to be rapid, specific, precise and accurate over the concentration range of 5 -150 µg.mL -1 and 1 -41 µg.mL -1 for ALK and HCT respectively and can be applied for the routine analysis of these drugs in bulk, and combined dosage form without any interference by the excipients.

Mean centering of double divisor ratio spectra, a novel spectrophotometric method for analysis of ternary mixtures

“…There are reported methods for the determination of AML, ALI or HCT in different dosage forms [27][28][29][30][31]. AML, ALI and HCT were simultaneously determined in their ternary mixture by HPLC and capillary electrophoresis [32,33].…”

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

“…Spectroscopic [8][9][10], High Performance Liquid Chromatography (HPLC) [11][12][13], Thin Layer Chromatography (TLC) [14], Electrochemical [15][16][17] and mass spectrometry [18] methods for estimation of MOX alone or in combination with other drugs in pharmaceutical preparation have been reported. Hydrochlorothiazide alone or in combination with other drugs is estimated by HPLC [19][20][21][22][23][24][25][26][27][28][29], TLC-densitometry [30][31][32][33][34][35][36], capillary electrophoresis [37][38][39], electrochemical [40][41][42][43] and spectroscopic methods [44][45][46][47][48][49][50][51][52][53]. Simultaneous estimation of MOX and HCT has been reported by RP-HPLC [54,…”

Simultaneous analysis of aliskiren and hydrochlorothiazide in pharmaceutical preparations and spiked human plasma by HPTLC

“…Several methods have been reported for the determination of ALS as an isolated sample or together with other antihypertensive drugs in more complicated matrices such as pharmaceutical formulations and biological fluids. These methods have been based on spectrophotometry [6][7][8], spectrofluorometry [9,10], electrophoresis [11,12], liquid chromatography (LC) [8,[13][14][15][16][17][18][19][20][21], LC-MS/MS [22][23][24][25] and UPLC-MS/MS [26]. Similarly, a number of analytical methods have been presented for the estimation of HCTZ as a single analyte [27] and in the presence of different antihypertensive agents [8,12,14,16,18,19,[28][29][30][31][32][33][34][35][36][37][38][39] in pharmaceutical preparations and biological matrices using spectrophotometry [29,32], capillary electrophoresis [28], HPTLC [30,…”