Mean centering of double divisor ratio spectra, a novel spectrophotometric method for analysis of ternary mixtures

Hassan, Said A.; Elzanfaly, Eman S.; Salem, Maissa Y.; El‐Zeany, Badr A.

doi:10.1016/j.saa.2015.07.109

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…The amplitude values of both active substances were determined using the regression equation for each component at its own selected wavelength [14][15][16][17].The obtained regression equation general formula is as follows: software can result the average value of the absorbance value of the ratio at each wavelength point. The result of this average value subtracted on the absorbance ratio is the MC value and is called the amplitude and plotted by the wavelength, and the regression equation is obtained [8,[11][12][13].…”

Section: Linearitymentioning

confidence: 99%

“…This method has supremacy than ordinary ultraviolet spectrophotometry and derivative spectrophotometry because without going through the derivatization process [6]. Some researchers have used MCR methods on the determination of mixed drugs such as thiomersal, lidocaine, and phenylephrine, ternary mixture of amlodipine, aliskiren, and hydrochlorothiazide, mixtures of diclofenac and sodium pantoprazole in tablets, gatifloxacin and dexamethasone in tablets, analysis of five-component mixture in drug analysis, salicylic acid and benzoic acid in fruit joice samples [7][8][9][10][11][12].The aim of this research is to a determination of SLF and TRM in tablet pharmaceutical form with 0.1 N NaOH as a solvent by MCR methods.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Simultaneous Sulfamethoxazole and Trimethoprim by Ultraviolet Spectrophotometry With Mean Centering of Ratio Spectra

Muchlisyam

Satiawan

2018

Asian J Pharm Clin Res

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Objective: Mean centering of ratio spectra (MCR) is analysis method for determination levels of mixture that has maximum wavelength is overlapping, and without preliminary separation. The purpose of this study is a determination of sulfamethoxazole (SLF) and trimethoprim (TRM) mixture by ultraviolet spectrophotometry with MCR.Methods: Absorption spectra of each drug were recorded and divided by the corresponding concentration of divisor, and the ratio spectra were then mean centered. SLF and t TRM concentrations were determined from calibration graphs by measuring at obtained maximum wavelengths 256 nm for SLF and 288 nm for TRM.Result: The result determination of SLF and TRM content in tablet S was 103.31±0.34% and 96.588±2.13%, respectively, result of validation method was fulfilled, percentage recovery for SLF was 101.24% and relative standard deviation (RSD) was 0.41%, and percentage recovery for TRM was 100.89% and RSD was 0.96%.Conclusion: The determination simultaneous by ultraviolet spectrophotometry of MCR was found to be accurate, precise, simple, and rapid method and can be used for SLF and TRM mixture.

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Section: Linearitymentioning

confidence: 99%