Application of cellulose/lignin hydrogel beads as novel supports for immobilizing lipase

Park, Saerom; Kim, Sung Hee; Kim, Ji Hyun; Yu, Hyejeong; Kim, Hyung Joo; Yang, Yung‐Hun; Kim, Hyungsup; Kim, Yong Hwan; Ha, Sung Ho; Lee, Sang Hyun

doi:10.1016/j.molcatb.2015.05.014

Cited by 73 publications

(19 citation statements)

References 41 publications

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“…The authors attributed the active effect of lignin to its rough and relatively well-developed surface, where numerous cavities ensure optimal distribution and stable contact between starch and the enzyme. Researchers observed that lignin-immobilized enzymes were characterized by higher catalytic efficiency and storage stability than their free counterparts [51,58,59]. The observations made in the earlier studies [25,26] and the results of the current microbiological and biochemical analyses lead to a similar conclusion.…”

Section: Bacterial Count and Enzymatic Activitymentioning

confidence: 71%

“…So far, other researchers have mainly focused on assessing the activation and stabilization of specific enzymes by lignin. For example, lignin was proved to activate α-amylase and lipase [58], where lipase could be immobilized on cellulose/lignin beads [59] or on the chitin/lignin composite [23]. Gong et al (2017) used lignin extracted from bamboo shoot shells (BBS) to activate and α-amylase immobilization [54].…”

Section: Process Performancementioning

confidence: 99%

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The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

et al. 2019

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The aim of the article was to present the effects of lignin grafted with polyvinylpyrrolidone (PVP) as a microbial carrier in anaerobic co-digestion (AcoD) of cheese (CE) and wafer waste (WF). Individual samples of waste cheese and wafers were also tested. The PVP modifier was used to improve the adhesive properties of the carrier surface. Lignin is a natural biopolymer which exhibits all the properties of a good carrier, including nontoxicity, biocompatibility, porosity, and thermal stability. Moreover, the analysis of the zeta potential of lignin and lignin combined with PVP showed their high electrokinetic stability within a wide pH range, that is, 4-11. The AcoD process was conducted under mesophilic conditions in a laboratory by means of anaerobic batch reactors. Monitoring with two standard parameters: pH and the VFA/TA ratio (volatile fatty acids-to-total alkalinity ratio) proved that the process was stable in all the samples tested. The high share of N-NH 4 + in TKN (total Kjeldahl nitrogen), which exceeded 90% for WF+CE and CE at the last phases of the process, proved the effective conversion of nitrogen forms. The microbiological analyses showed that eubacteria proliferated intensively and the dehydrogenase activity increased in the samples containing the carrier, especially in the system with two co-substrates (WF+CE/lignin) and in the waste cheese sample (CE/lignin). The biogas production increased from 1102.00 m 3 Mg −1 VS (volatile solids) to 1257.38 m 3 Mg −1 VS in the WF+CE/lignin sample, and from 881.26 m 3 Mg −1 VS to 989.65 m 3 Mg −1 VS in the CE/lignin sample. The research results showed that the cell immobilization on lignin had very positive effect on the anaerobic digestion process.

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Section: Bacterial Count and Enzymatic Activitymentioning

confidence: 71%

Section: Process Performancementioning

confidence: 99%

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

et al. 2019

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“…Later, Kim and co‐workers reported the accomplishment of lipase activation through lyophilization with ionic liquids or ionic surfactants [45–48]. Koo and co‐workers also reported efficient activation of lipase through lyophilizaton of ionic liquid type sol‐gel silica [49]; similar activation methods have been demonstrated by several groups [50–57].…”

Section: Introductionmentioning

confidence: 79%

Phosphonium alkyl PEG sulfate ionic liquids as coating materials for activation of Burkholderia cepacia lipase

Matsubara

Kadotani

Nishihara

et al. 2015

Biotechnology Journal

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Lipases are among the most widely used enzymes applicable for various substrates; however, the slow reactions or poor enantioselective reactions are sometimes obtained. To develop ionic liquid type activating agents for lipase, four types of phosphonium cetyl(PEG)10 sulfate ionic liquids have been synthesized and used as coating materials of Burkholderia cepacia lipase (Lipase PS) through the lyophilization process. Tributyl ([2-methoxy]ethoxymethyl)phosphonium cetyl(PEG)10 sulfate ([P444MEM ][C16 (PEG)10 SO4 ]) (PL1) worked best among them, and PL1-coated lipase PS displayed high reactivity in transesterification of broad types of secondary alcohols using vinyl acetate as an acylating reagent with perfect enantioselectivity (E > 200). The substrate preference of PL1-PS differs from that of commercial lipase PS or [bdmim] [C16 (PEG)10 SO4 ]-coated lipase (IL1-PS); PL1-PS displayed excellent enantioselectivity in the reaction of 2-chloro-1-phenylethanol with E > 200, though insufficient E values were recorded for lipase PS (E = 12) and IL1-PS (E = 123) for this alcohol. PL1-PS also showed perfect enantioselectivity (E > 200) for the reaction of 1-(pyridin-2-yl)ethanol, while IL1-PS showed E = 130 for this compound. We further succeeded in demonstrating the recyclable use of PL1-PS five times in tributyl(3-methoxypropyl)phosphonium bis(trifluoromethylsulfonyl)amide ([P444PM ][Tf2 N]) as a solvent. Since PL1-PS is easily applicable to 10-20 gram-scaled reactions, it is expected that the IL-coated enzyme might be useful for practical preparation of a wide variety of chiral secondary alcohols.

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“…In recent years, porous cellulose beads (CBs) derived from regenerated cellulose are receiving intense research interest for their potential biomedical and biotechnological applications such as chromatography system [1], immobilization of enzymes [2], and drug delivery system [3]. CBs are potentially cost-effective drug delivery carriers due to their high porosity and high specific surface area, favourable drug loadings, and release profiles under specific conditions [4].…”

Section: Introductionmentioning

confidence: 99%

Porous Cellulose Beads Fabricated from Regenerated Cellulose as Potential Drug Delivery Carriers

Voon

Pang

Chin

2017

Journal of Chemistry

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Highly porous cellulose beads (CBs) of various mean sizes were successfully prepared from regenerated cellulose of paper wastes. The drug delivery characteristics of CBs with different mean sizes were investigated using curcumin as the model drug under controlled conditions. The loading capacity and efficiency of curcumin onto CBs were substantially influenced by factors such as their morphological characteristics, curcumin concentration, and duration of loading. The release kinetic profiles of curcumin from CBs of different mean sizes were investigated in media of pH values resembling digestive juices and intestinal fluids. Release kinetic models were used to simulate and elucidate release kinetics and mechanisms of curcumin from CBs under specific conditions. The loading capacity and efficiency of curcumin onto CBs could be enhanced via the optimization of curcumin solution concentration and the morphological characteristics of CBs, whereas the release kinetic profiles of curcumin from CBs could be modulated by varying the mean diameter of CBs. Optimized CBs derived from regenerated cellulose of paper wastes are potentially useful as cost-effective drug delivery carriers.

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Application of cellulose/lignin hydrogel beads as novel supports for immobilizing lipase

Cited by 73 publications

References 41 publications

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

Phosphonium alkyl PEG sulfate ionic liquids as coating materials for activation of Burkholderia cepacia lipase

Porous Cellulose Beads Fabricated from Regenerated Cellulose as Potential Drug Delivery Carriers

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