The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

Pilarska, Agnieszka A.; Wolna-Maruwka, Agnieszka; Pilarski, Krzysztof; Janczak, Damian; Przybył, Krzysztof; Gawrysiak‐Witulska, Marzena

doi:10.3390/polym11122073

Cited by 18 publications

(28 citation statements)

References 53 publications

(98 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are both positive and negative effects of increased concentration of Ca and Na on the operation of an anaerobic reactor [41,42]. Excessive levels of calcium may cause the formation of mill scale, however, regarding the amount of calcium in cheese waste [24] and applied to the reactor, that macronutrient may contribute to the formation of cell aggregates. Salt (including sodium), which can be found in cheese waste in an anaerobic system, may negatively impact the microorganism activity and disrupt their metabolism.…”

Section: Substrate Characteristicsmentioning

confidence: 99%

“…The rapid return to a state of full activity of methanogens did not adversely affect the results of biogas production. A decline in VFA/TA ratio indicates effective decomposition and removal of organic matter [13,24].…”

Section: Stability and Performance Of Anaerobic Digestionmentioning

confidence: 99%

“…In turn, lignin, a natural polymer, is the primary wood component; it has a porous structure and shows hydrolytic enzyme resistance and thermal stability [22]. In recent years, the possibilities to use lignin as a potential microbe carrier have been explored in organic waste mono-digestion and co-digestion as described by Pilarska et al (2018 [23,24]. The silica/lignin material was tested by as a way of anaerobic digestion of sewage sludge (SS) [25].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters

et al. 2021

Self Cite

View full text Add to dashboard Cite

The article aims to present results of research on anaerobic digestion (AD) of waste wafers (WF-control) and co-substrate system–waste wafers and cheese (WFC-control), combined with digested sewage sludge, as inoculum. The purpose of this paper is to confirm the outcome of adding silica/lignin (S/L; 4:1) material, as a microbial carrier, on the process performance and genetic diversity of microbial communities. The experiment was conducted in a laboratory under mesophilic conditions, in a periodical operation mode of bioreactors. Selected physicochemical parameters of the tested carrier, along with the microstructure and thermal stability, were determined. Substrates, batches and fermenting slurries were subjected to standard parameter analysis. As part of the conducted analysis, samples of fermented food were also tested for total bacterial count, dehydrogenase activity. Additionally, DNA extraction and next-generation sequencing (NGS) were carried out. As a result of the conducted study, an increase in the volume of produced biogas was recorded for samples fermented with S/L carrier: in the case of WF + S/L by 18.18% to a cumulative biogas yield of 833.35 m3 Mg−1 VS, and in the case of WFC + S/L by 17.49% to a yield of 950.64 m3 Mg−1 VS. The largest total bacterial count, during the process of dehydrogenase activity, was maintained in the WFC + S/L system. The largest bacterial biodiversity was recorded in samples fermented with the addition of cheese, both in the case of the control variant and in the variant when the carrier was used. In contrast, three phyla of bacteria Firmicutes, Proteobacteria and Actinobacteria predominated in all experimental facilities.

show abstract

Section: Substrate Characteristicsmentioning

confidence: 99%

Section: Stability and Performance Of Anaerobic Digestionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The anaerobic digestion process was conducted in a periodic mode of operation of digesters, under mesophilic conditions. The authors of this study presented a detailed diagram and described the construction and operation of biodigesters in their previous publications [32][33][34].…”

Section: Laboratory-scale Biogas Productionmentioning

confidence: 99%

The Efficiency of Industrial and Laboratory Anaerobic Digesters of Organic Substrates: The Use of the Biochemical Methane Potential Correction Coefficient

et al. 2020

Self Cite

View full text Add to dashboard Cite

This study is an elaboration on the conference article written by the same authors, which presented the results of laboratory tests on the biogas efficiency of the following substrates: maize silage (MS), pig manure (PM), potato waste (PW), and sugar beet pulp (SB). This article presents methane yields from the same substrates, but also on a technical scale. Apart from that, it presents an original methodology of defining the Biochemical Methane Potential Correction Coefficient (BMPCC) based on the calculation of biomass conversion on an industrial scale and on a laboratory scale. The BMPCC was introduced as a tool to enable uncomplicated verification of the operation of a biogas plant to increase its efficiency and prevent undesirable losses. The estimated BMPCC values showed that the volume of methane produced in the laboratory was overestimated in comparison to the amount of methane obtained under technical conditions. There were differences observed for each substrate. They ranged from 4.7% to 17.19% for MS, from 1.14% to 23.58% for PM, from 9.5% to 13.69% for PW, and from 9.06% to 14.31% for SB. The BMPCC enables estimation of biomass under fermentation on an industrial scale, as compared with laboratory conditions.

show abstract

“…The anaerobic bioreactor (12-chamber section) used in the biogas production experiment: 1-water heater; 2-water pump; 3-insulated tubes for heating medium; 4-water jacket (39 • C); 5-bioreactor (1.4 L); 6-slurry sampling valve; 7-tube for biogas transport; 8-graduated tank for biogas; 9-gas sampling valve[40].…”

mentioning

confidence: 99%

The Influence of the Process of Sugar Beet Storage on Its Biochemical Methane Potential

et al. 2020

Self Cite

View full text Add to dashboard Cite

The manner of storage of sugar beets largely influences their physical and chemical properties, which may subsequently determine their biochemical methane potential. In this study, samples of fresh sugar beets as well as beets stored in two ways—in airtight conditions and in an open-air container—were tested. In both cases, measurements were taken on specific dates, i.e., after 4, 8, 16 and 32 weeks of storage. A decrease in pH was observed in all samples, with the lowest decrease occurring in hermetically stored samples. The lowest pH value of 3.71 was obtained for sugar beets stored in an open-air container after 32 weeks of storage. During storage, a gradual decrease in total solids was also recorded along with accompanying losses of organic matter, more significant in the case of storage in an open-air container. In subsequent storage periods, the biogas/methane production efficiency differed slightly for both methods. The highest volume of biogas was obtained for fresh sugar beets—148.23 mL·g−1 fresh matter (FM)—and subsequently in the 8th and 16th weeks of storage: 139.35 mL·g−1 FM (H—airtight conditions) and 144.14 mL·g−1 FM (O—open-air container), and 147.58 H mL·g−1 FM (H) and 148.22 mL·g−1 FM (O), respectively. The storage period affected the time of anaerobic decomposition of the organic matter—fresh sugar beets took the longest to ferment (26 days), while the material stored for 32 weeks took the shortest to ferment. In the experiment, the content of selected organic compounds in individual samples, i.e., sugar, methanol, ethanol, lactic acid and acetic acid, was also analysed. Within these results, significant differences were found between the samples stored using the two different methods. A high content of sugar, methanol, ethanol and other chemical compounds in the “O” materials showed the hydrolysis and acidogenesis processes taking place in an open-air container, with the participation of catalytic microorganisms.

show abstract

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

Cited by 18 publications

References 53 publications

Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters

Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters

The Efficiency of Industrial and Laboratory Anaerobic Digesters of Organic Substrates: The Use of the Biochemical Methane Potential Correction Coefficient

The Influence of the Process of Sugar Beet Storage on Its Biochemical Methane Potential

Contact Info

Product

Resources

About