Application of Bio P60 and Bio T10 Alone or in Combination Against Stem Rot of Pakcoy

Soesanto, Loekas; Hiban, Atsil; Suharti, Woro Sri

doi:10.33089/jthort.v2i2.20

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…The secondary metabolites of P. fluorescens P60 was also able to inhibit the growth of R. solani. This is in accordance with the statement of Soesanto et al (2019), P. fluorescens P60 was able to delay the incubation period of disease, reduce disease incidence, and reduce the number of end pathogens.…”

Section: Effect Of P Fluorescens P60 Secondary Metabolites Enriched With Chitosan On the Components Of The Pathosystemsupporting

confidence: 91%

“…The high yield weight was in line with the low disease intensity which proved that the secondary metabolites was able to suppress development of the pathogens. As stated by Soesanto et al (2019), antagonists can stimulate plant growth and can also increase plant-induced resistance. The use of P. fluorescens P60 is very effective in accelerating seed germination and seed vigor so that it will affect the level of plant development and further to plant fresh weight.…”

Section: Effect Of the Secondary Metabolites Enriched With Chitosan On The Growth Componentsmentioning

confidence: 99%

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Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

2021

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Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.

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Section: Effect Of P Fluorescens P60 Secondary Metabolites Enriched With Chitosan On the Components Of The Pathosystemsupporting

confidence: 91%

Section: Effect Of the Secondary Metabolites Enriched With Chitosan On The Growth Componentsmentioning

confidence: 99%

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

2021

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“…The mixture was sterilized using an autoclave at a temperature of 121 ºC, a pressure of 15 psi for 30 min. After the mixture was cooled, each pure culture of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16, was taken from the PDA medium with a cork drill (10 mm in diameter) and each of 5 cork drills was put into the mixture and shaken with a shaker at 150 rpm at room temperature for 10 days (Soesanto et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Cross Application of Entomopathogenic Fungi Raw Secondary Metabolites for Controlling Fusarium Wilt of Chili Seedlings

Soesanto

Sari

Mugiastuti

et al. 2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Cross application of entomopathogenic fungi raw secondary metabolites for controlling fusarium wilt of chili seedlings. Theresearch aimed to determine the effect of entomopathogenic fungi raw secondary metabolites on fusarium wilt on chili plants and on growth of chili. In vitro test used a Completely Randomized Design with 5 treatments and 5 replicate and in planta using a Randomized Block Design with 5 treatments and 5 replicatie including control, secondary metabolites of Beauveria bassiana B10, B. bassiana B16, Metarhizium anisopliae M16, dan Lecanicillium lecanii L16. Variables observed included inhibition ability, incubation period, desease intensity, plant height, root length, and phenolic compounds (tannins, saponin, and hydroquinone) content qualitatively. The results showed that secondary metabolites of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16 were able to inhibit growth of Fusarium oxysporum f.sp. capsici by 50.62; 50,64; 48,62; 56.62%, respectively, extend incubation periods of 71.05; 73,38; 64.89; and 68.57%, respectively, suppress disease intensity by 99.99; 99.99; 99.99; and 99.99%, respectively, can increase plant height by 15.22; 18.8; 21.14; 21.69%, respectively, increasing the root length by 22.61; 25,71; 26,34; 33.50%, respectively, and can increase the content of tannins, saponins and hydroquinone compounds qualitatively compared to controls. The secondary metabolites of enthomopathogenic fungi could be used as organic control for soilborne pathogenic fungi.

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“…Production loss of horticulture crops in Serang Village is suggested because of pathogenic fungal infection. It has been reported by Soesanto et al (2019) that pathogenic fungi caused horticulture production loss.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Pathogenic Fungi and Disease on Vegetable Crops at Polyculture Systems

Sucianto

Abbas

2021

J Bio Bio Edu

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Vegetables polyculture system is potentially increasing pathogenic fungi diversity because various plant hosts are available. There is no data about patogenic fungi diversity at polyculture vegetable farming in Serang village, District of Karangreja, Purbalingga Regency. This study aimed to determine patogenic fungal diversity and disease percentage caused by the fungi at polyculture vegetable farming in Serang village, District of Karangreja, Purbalingga Regency. This research used purposive random sampling. Infected plants were collected at ten polyculture farming locations and fungal identification was performed at the laboratory. Fungi were identified morphologically based on the signs, symptoms, as well as macroscopic and microscopic characters. The fungi's pathogenity was determined by applying Koch's postulate test. The data were analyzed descriptively through literature comparison. The results showed that seven fungal species were found at polyculture farms in Serang Village. The obtained fungi were Colletotrichum sp., Fusarium sp., Alternaria sp., Septoria sp., Cercospora sp., Botryodiplodia sp., and Nigrospora sp. The lowest damage was 18.24% on tomato fruit infected by Fusarium sp. and the highest was on chili plants which was caused by Colletotrichum sp. The data is the first report for polycuture system. The obtained data has important implication for the management of vegetables farming in Serang Village.

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Application of Bio P60 and Bio T10 Alone or in Combination Against Stem Rot of Pakcoy

Cited by 5 publications

References 26 publications

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

Cross Application of Entomopathogenic Fungi Raw Secondary Metabolites for Controlling Fusarium Wilt of Chili Seedlings

Diversity of Pathogenic Fungi and Disease on Vegetable Crops at Polyculture Systems

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