Application of an ultra-performance liquid chromatography-tandem mass spectrometric method for the detection and quantification of cannabis in cerumen samples

Phenolics from Defatted Black Cumin Seeds (Nigella sativa L.): Ultrasound-Assisted Extraction Optimization, Comparison, and Antioxidant Activity

“…The linear ranges for each analyte are represented in Table 1 . The limits of detection (LOD) and limit of quantification (LOQ), used to assess the sensitivity of the method, were calculated based on the standard deviation of the response (σ) and the slope of the corresponding calibration curve [ 22 ]. The detection limits were expressed as: LOD = 3.3 σ/Slope LOQ = 10 σ/Slope …”

HPLC and 32P‐radiolabeling method for quantification of microbial adenylate concentrations and turnover rates in seawater

“…Calibration curve linearity was calculated by the correlation coefficients ( R 2 ) from modeled linear regressions (R Core Team 2020). The limit of detection (LOD) and limit of quantification (LOQ) were calculated from the standard deviation of the calibration curve residuals ( S y ) and the slope of the nonradiolabeled calibration curve ( m n ), according to the formulas LOD = 3.3*( S y / m ) and LOQ = 10*( S y / m ) (Nicolaou et al 2021). For samples that are at or below the LOQ for adenylate concentrations, an internal standard can be used to increase the signal for quantification, either using the addition of a single concentration of internal standard (typically adding 10 μ L or less of a mixed standard of ATP, ADP, and AMP) where samples are then quantified using an external standard curve, or standard addition to several subsets of a sample at multiple concentrations enabling calculation of concentrations from the response within samples (Strehler 1968; Karl 1993b; Harris and Lucy 2020; Bochdansky et al 2021).…”

Cancer evaluation in dogs using cerumen as a source for volatile biomarker prospection

“…It refers to secretions of the sebaceous and sweat glands containing polar and non-polar substances, mainly metabolic lipid-derived components. 16,17 Recently, cerumen VOMs have been successfully applied in diabetes diagnosis, 18,19 forensic applications, [20][21][22][23][24] toxicological monitoring purposes, [25][26][27][28][29] identification of rare otolaryngological disorder (Me ´nie `re's disease), 30 and human cancer diagnosis. 31 The chemical fingerprint of VOMs for use in disease detection is known as Cerumenogram.…”