Application of an orcinol-ferric chloride colorimetric assay in barley and wheat accessions for water-extractable and total arabinoxylan

Hu, Gongshe; Ellberg, S.; Burton, C. L.; Satterfield, K.; Bockelman, Harold E.

doi:10.1016/j.jcs.2020.102962

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…The leaf samples were treated according to the analytical procedure recommended by the national renewable energy laboratory (NREL) [ 80 ] to obtain the filtrate. The content of hemicellulose in the filtrate was determined by the orcinol colorimetric method [ 81 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Yuan

Liu

Takáč

et al. 2021

Plants

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The cell wall plays an important role in responses to various stresses. The cellulose synthase-like gene (Csl) family has been reported to be involved in the biosynthesis of the hemicellulose backbone. However, little information is available on their involvement in plant tolerance to low-temperature (LT) stress. In this study, a total of 42 Csls were identified in Musa acuminata and clustered into six subfamilies (CslA, CslC, CslD, CslE, CslG, and CslH) according to phylogenetic relationships. The genomic features of MaCsl genes were characterized to identify gene structures, conserved motifs and the distribution among chromosomes. A phylogenetic tree was constructed to show the diversity in these genes. Different changes in hemicellulose content between chilling-tolerant and chilling-sensitive banana cultivars under LT were observed, suggesting that certain types of hemicellulose are involved in LT stress tolerance in banana. Thus, the expression patterns of MaCsl genes in both cultivars after LT treatment were investigated by RNA sequencing (RNA-Seq) technique followed by quantitative real-time PCR (qPCR) validation. The results indicated that MaCslA4/12, MaCslD4 and MaCslE2 are promising candidates determining the chilling tolerance of banana. Our results provide the first genome-wide characterization of the MaCsls in banana, and open the door for further functional studies.

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Section: Methodsmentioning

confidence: 99%