Application of a multi-sulfonamide biosensor immunoassay for the detection of sulfadiazine and sulfamethoxazole residues in broiler serum and its use as a predictor of the levels in edible tissue

Haasnoot, W.; Bienenmann-Ploum, M.E.; Lamminmäki, Urpo; Swanenburg, M.; Rhijn, Hans van

doi:10.1016/j.aca.2005.07.053

Cited by 37 publications

(20 citation statements)

References 18 publications

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“…In literature many papers concern the development of an immunoassay for the sensitive detection of antibiotic residues in food samples [20 -23]; several works are based on a biosensing approach with an optical transduction [5,24,25] and in our knowledge, only one work regarding the development of an electrochemical immunosensor for sulfonamides detection based on the use of magnetic beads is reported [6]. This device is based on the use of antibodies against sulfonamide antibiotics, tosyl-activated magnetic beads and electrochemical detection by graphite-epoxy composite (m-GEC) electrodes.…”

Section: Introductionmentioning

confidence: 99%

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

et al. 2010

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In this work an electrochemical immunoassay, based on a direct competitive assay, was developed using magnetic beads as solid phase and carbon screen-printed arrays as transducers for the detection of sulfonamides in food matrices such as honey. Magnetic beads coated with protein A were modified by immobilisation of specific antibodies and then the competition between the target analyte and the corresponding analyte-labelled with an enzyme was carried out; after the immunosensing step, beads were captured by a magnet onto the working surfaces of a screenprinted eight-electrodes array for a multiple electrochemical detection. Screen-printed eight-electrodes arrays were chosen as transducers due to the possibility to repeat multiple analysis and to test different samples simultaneously. Alkaline Phosphatase (AP) was used as enzyme label and Differential Pulse Voltammetry (DPV) as fast electrochemical technique. Calibration curves demonstrate that the developed electrochemical immunoassay was able to detect this class of drugs in standard solutions at low concentrations (ng/mL levels). The short incubation times (25 min) and the fast electrochemical measurement (10 sec) make of these systems a possible alternative to classic ELISA tests. The developed electrochemical immunoassay was coupled to Solid Phase Extraction (SPE) for the analysis of these compounds in a food matrix such as honey.

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Section: Introductionmentioning

confidence: 99%

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

et al. 2010

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“…Nowadays, one of the most rapid immunoassay technologies applies a commercially available automated surface plasmon resonance (SPR)-based biosensor (Biacore). For the fast detection of sulfonamides (5-10 min per sample), applications were described for the determination of sulfamethazine residues in milk [6,7], the detection of sulfamethazine and sulfadiazine in pig bile [8,9] and for the multi-sulfonamide detection in chicken serum [10][11][12] in which a recombinant multisulfonamide antibody (M.3.4) was selected as most sensitive towards most of the sulfonamides tested [11][12][13]. However, these biosensors are expensive, limited in high volume testing (6-12 samples per hour) and limited in multiplex detection (simultaneous measurements can be performed in 1-4 flow channels).…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric immunoassay for sulfonamides in raw milk

Keizer

Bienenmann-Ploum²,

Bergwerff

et al. 2008

Analytica Chimica Acta

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“…To date, most multi-analyte assays for veterinary drug residues have focused on structurally related compounds [17][18][19][20][21][22], and no method has been developed for the simultaneous detection of different drug classes in animal tissue samples.…”

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confidence: 99%

A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

et al. 2011

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A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples, using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+ ) and samarium (Sm 3+ ), respectively. The detection limits for CAP and RAC were 0.06 and 0.25 ng/g. The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5 ng/g, and 69.8%-85.8% for RAC at spiking levels of 1-10 ng/g. The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS, with correlation coefficients (R) between 0.92-0.98. dual-label time-resolved fluoroimmunoassay, chloramphenicol, ractopamine, residue, swine tissue Citation:Zhang Z, Liu J F, Yao Y, et al. A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue.

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Application of a multi-sulfonamide biosensor immunoassay for the detection of sulfadiazine and sulfamethoxazole residues in broiler serum and its use as a predictor of the levels in edible tissue

Cited by 37 publications

References 18 publications

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

Flow cytometric immunoassay for sulfonamides in raw milk

A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

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