A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

Zhang, Zhen; Liu, Jing Fu; Yao, Yan; Jiang, Gui Bin

doi:10.1007/s11434-011-4412-4

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…Lanthanide chelates have unique fluorescent properties, such as high quantum yields, narrow band emission peak, long stoke shift, and fluorescence lifetime. Time-resolved fluoroimmunoassay (TRFIA) has higher sensitivity than most of the conventional methods, while they decrease the matrix interference in the assay [26,30]. An Eu 3+ fluorescent lanthanide-based TRFIA for detecting T&T in edible tissues is described, which offers cost and efficiency advantages, as well as simplified analysis steps [31].…”

Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Wang

Yang

et al. 2021

Foods

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Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.

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Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Wang

Yang

et al. 2021

Foods

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“…Time-resolved fluoroimmunoassay, which offers the advantages of rapidity, simplicity, high sensitivity, and specificity, and simultaneous detection of many indicators, has been successfully developed for detection of mycotoxin, antibiotics, hormones, veterinary drugs, diseases, pesticides, and halogenated or aromatic pollutants for agricultural, food, medical, and environmental analysis Zhang et al 2011;Zhao et al 2009;Zhang et al 2010;Bacigalupo et al 2007). Its sensitivity, measurement range, and stability are substantially superior to those of the existing enzyme-linked immunosorbent assay (ELISA, L. L. Cheng et al 2013;Yue et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Development of a Time-Resolved Fluoroimmunoassay for the Rapid Detection of Methyl-3-Quinoxaline-2-Carboxylic Acid in Porcine Tissues

Wei

Niu

et al. 2014

Analytical Letters

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A time-resolved fluoroimmunoassay for the specific determination of methyl-3-quinoxaline-2-carboxylic acid in animal tissues, a marker residue of olaquindox, was developed. The IC 50 of the assay was found to be 1.46 ± 0.19 ng/mL of methyl-3-quinoxaline-2-carboxylic acid in phosphate-buffered saline samples and the detection limit was 0.16 ± 0.03 ng/mL. For porcine liver and muscle samples spiked with 5, 10, and 15 ng/g, the recovery ranges were 95.7-112.3% and 98.5-116.2% and the coefficients of variation were 9.3-11.5% and 8.9-14.2%, respectively. The time-resolved fluoroimmunoassay results correlated well with high performance liquid chromatography results (correlation coefficients of 0.991 for liver and 0.988 for muscle). This study suggests that this method is simple, fast, and sensitive for the high-throughput determination of methyl-3-quinoxaline-2-carboxylic acid in animal tissues.

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“…The half-life of background fluorescence is very short so the long duration of lanthanide chelate emission allows longer measurement, which distinguishes specific fluorescence from the background. Thus, the sensitivity of a TRFIA is higher than that of conventional methods, which lessens the susceptibility of the TRFIA to matrix interference [21,23]. This is the first report describing an Eu 3+ fluorescent lanthanides-based TRFIA with high sensitivity and costefficiency for detection of tylosin and tilmicosin in edible tissues of food-producing animals.…”

mentioning

confidence: 99%

Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

Wei

Chen

et al. 2013

Chin. Sci. Bull.

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To quantitatively determine tylosin and tilmicosin in edible animal tissues, a time-resolved fluoroimmunoassay (TRFIA) has been developed and validated. For this purpose, desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates, standards and samples were loaded and, finally, diluted europium-labeled anti-tylosin antibodies were added. Results show that the limit of detection for tylosin was 0.03 ng mL −1 and that for tilmicosin was 0.05 ng mL −1 . The recoveries were 73.6% to 120.5%, with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ng g −1 . There was good correlation (R 2 >0.99) between the TRFIA, an enzyme-linked immunosorbent assay and high performance liquid chromatography data. In conclusion, the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening. The method is expected to be widely applicable.

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A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

Cited by 6 publications

References 26 publications

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Development of a Time-Resolved Fluoroimmunoassay for the Rapid Detection of Methyl-3-Quinoxaline-2-Carboxylic Acid in Porcine Tissues

Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

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