Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants

Kusznierewicz, Barbara; Piasek, Anita; Bartoszek, Agnieszka; Namieśnik, Jacek

doi:10.1016/j.jfca.2011.01.010

Cited by 31 publications

(26 citation statements)

References 17 publications

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“…Profiles of antioxidants present in studied berry fruits were obtained by HPLC-DAD (Agilent Technologies, Santa Clara, CA, USA) coupled with post-column derivatization with ABTS reagent using a Pinnacle PCX Derivatization Instrument (Pickering Laboratories, Inc., Mountain View, CA, USA) according to the procedure described previously [21,22].…”

Section: Profiles Of Antioxidants and Antioxidant Capacitymentioning

confidence: 99%

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The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins

Koss-Mikołajczyk

Kusznierewicz

Bartoszek

2019

Foods

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The aim of this research was to correlate the composition of phenolic compounds and bioactivities (antioxidant, cytotoxic, antigenotoxic, and influence on selected enzymatic activities) exhibited by extracts from differently pigmented raspberry (yellow and red), grape (white and red), mulberry (white and black), and currant (white, red, and black) varieties. It was presumed that phytocomplexes of the same species will be similar while differing significantly only by the content of anthocyanins in colored varieties, which will impact biological properties. Additionally, to compare food matrix embedded and isolated anthocyanins, the influence of purified solution, in concentrations matching the total concentration of anthocyanins in appropriate colored plant samples, on investigated biological activities was studied. The phenolic compound composition and antioxidant profiles showed that anthocyanin content was correlated only with antioxidant capacity of tested plant extracts. The other determined biological activities failed to reveal any simple relationship between chemopreventive potential and anthocyanin composition in fruits studied nor any similarity to biological properties of isolated cyanidin-3-O-glucoside. These observations indirectly support the food synergy concept, that points to interactions between different phytochemicals as a factor deciding about the final bioactivity of edible plants.

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Section: Profiles Of Antioxidants and Antioxidant Capacitymentioning

confidence: 99%

“…Standard spectrophotometric methods (ABTS, DPPH, and FC) were used to determine antioxidant activity of tested fruit extracts as described earlier [22,23]. Obtained results were expressed as Trolox Equivalents (TE (µmol/g d.w.)).…”

Section: Profiles Of Antioxidants and Antioxidant Capacitymentioning

confidence: 99%

The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins

Koss-Mikołajczyk

Kusznierewicz

Bartoszek

2019

Foods

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“…Over the past two decades, a number of analytical methods measuring total antioxidative activity have been developed, one of them based on the ability of an antioxidant to quench ABTS radicals. The same chemical reaction has been exploited for online HPLC‐coupled method that enables profiling of antioxidants in complex mixtures following their chromatographic separation from the matrix . As in ABTS colorimetric assays, the reduction reaction leads to a significant shift in the UV–visible spectrum, so the change in absorption – discolouring of ABTS reagent – can serve as a quantitative measure of antioxidative potential of analytes separated by HPLC.…”

Section: Resultsmentioning

confidence: 99%

“…The profiling of antioxidants in apple extracts was performed by post‐column derivatisation as described earlier with slight modifications. The post‐column addition of ABTS derivatisation reagent to HPLC eluate was performed using a Pinnacle PCX derivatisation instrument (Pickering Laboratories Inc., Mountain View, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

The influence of plant protection by effective microorganisms on the content of bioactive phytochemicals in apples

et al. 2017

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“…The HPLCcolumn used was a C18 column, 150 mm in length and 4.6 mm in internal diameter. The mobile phase was acetonitrile: water (70: 30) [13][14][15]. The UV detector was operated at a wavelength of 220 nm.…”

Section: Methodsmentioning

confidence: 99%

Remediation of contaminated water from nitrate and diazinon by nanofiltration process

Mahmoodi

Borazjani

Farhadian

et al. 2014

Desalination and Water Treatment

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Nitrate and diazinon pesticide is among the environmental challenges which enter water resources, mostly as a result of agricultural activities. In this study, the effects of diazinon concentration, nitrate concentration and pH on the efficiency of simultaneous removal of contaminants from polluted water were investigated, applying a commercial polyamide nanofilter. Each factor was considered in three levels where diazinon concentration, nitrate concentration and solution pH were in the range of 10-1,000 μg/L, 40-160 mg/L and 5-9, respectively. The experiments were conducted at constant pressure of 6 bar. The response surface method was adopted in the experimental design to obtain the impact of mentioned factors. It was found that increasing diazinon concentration and pH enhance the pesticide removal efficiency up to 94%, while increasing nitrate concentration increases the commercial nanofilter efficiency from 80 to 85%. The diazinon removal percentage at optimum condition was estimated to be about 93% at diazinon concentration of about 90 μg/L, nitrate concentration of about 80 mg/L and pH of 9.

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Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants

Cited by 31 publications

References 17 publications

The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins

The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins

The influence of plant protection by effective microorganisms on the content of bioactive phytochemicals in apples

Remediation of contaminated water from nitrate and diazinon by nanofiltration process

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