Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates

Abstract

Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The preparation is obtained according to the original technology through maceration of animal endocrine raw materials, followed by purification, concentration, isolation and freeze dehydration of the enzyme complex. The results of the laboratory studies of the proteolytic activity of enzyme preparations show that Protepsin, the preparation of animal origin with a proteolytic activity of 100 units/g, has the greatest collagenase activity in the biomodification of native collagen-containing raw materials.

Obtaining and estimating the potential of protein nutraceuticals from highly mineralized collagen-containing beef raw materials

“…Initially, the most effective enzyme preparation and rational regimes of its use for enzymolysis of meat and bone raw materials were selected. This would allow maximum transformation of highly mineralized high molecular weight proteins from beef bones into the dissolved state [12,13,14,17,19,20,21,22]. The results of the experiments carried out using various enzyme preparations with varied doses are presented in Table 1.…”

“…In the latter case, they can be especially in demand among the elderly, sportspersons, workers with increased physical loads and others. The use of low molecular weight protein nutraceuticals is promising in the composition of several protein multicomponent BAAs as a source of active peptides intended for compensation of deficiency in the amino acids -neurotransmitters (glycine, alanine) and others increasing physiological and immune functions of the body [1,2,13,17,18,24,27].…”

“…Recovery of valuable organic components from secondary meat and bone raw materials is based on their destruction, which is performed by different methods. The main methods for degradation of highly mineralized raw materials are based on tough hydrolysis, which is carried out using chemical reagents (acids, alkali) as well as by enzymatic, thermal or combined ways [2,17,18,19,20,21,22,23]. However, acids and alkali destroy several valuable amino acids, reduce the biological value of proteins and peptides, hydrolyse lipids.…”

“…It is also necessary to note that the investigated enzymatic methods for protein production from meat and bone raw materials are not effective enough for industrial use of end products of hydrolysis in food systems [17,18,19]. It was established that enzymatic hydrolysates of animal raw material waste often contain emulsion-type protein-fat mixtures, which are often suitable only as a biodiesel fuel, as well as in the composition of feedstuff for animals, fertilizers for soil, as a carbohydrate and nitrogen source for microbial synthesis.…”

Characterization of Fish Gelatin Obtained from Atlantic Cod Skin Using Enzymatic Treatment

“…A more advanced approach was patented, which uses proteolytic enzymes produced by nonpathogenic bacteria [26]. It was shown that the enzyme preparation protosubtilin has higher collagenase activity in the temperature range 35-45 • C compared to papain, which exhibits maximum activity at temperatures close to the temperature of protein denaturation [27].…”