Appearance of Drug Resistance Mutations Among the Dominant HIV-1 Subtype, CRF01_AE in Maumere, Indonesia

Indriati, Dwi Wahyu; Kotaki, Tomohiro; Khairunisa, Siti Qamariyah; Witaningrum, Adiana Mutamsari; Matondang, Muhammad Qushai Yunifiar; Ueda, Shuhei; Nasronudin, -; Purnama, Asep; Kurniawan, Dwi Agus; Kameoka, Masanori

doi:10.2174/1570162x16666180502114344

Cited by 10 publications

(19 citation statements)

References 41 publications

(43 reference statements)

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“…The pattern of resistance-associated mutations found in this study differs to that found in Maumere, East Nusa Tenggara, except for M184V [11]. This discrepancy might relate to the subject of the genotypic study.…”

Section: Discussioncontrasting

confidence: 91%

“…As expected, almost all of the HIV-1 identified individuals in this study harbored CRF01_AE subtype, which is the most common circulating subtype in Indonesia [9, 11, 24]. Only one was of A subtype.…”

Section: Discussionsupporting

confidence: 76%

“…This discrepancy might relate to the subject of the genotypic study. We were targeting naïve as well as treatment failure patients, while the other study did not explain if the subjects were naïve or treatment failure patients [11]. The different time frame of sampling might also cause this dissimilar observation.…”

Section: Discussionmentioning

confidence: 99%

“…This is particularly valid for the HIV-B subtype that predominantly circulates in America, Europe, and Australia [6]. However, the most globally prevalent subtype is HIV-1 C [7, 8], and the most widespread circulating subtype in Indonesia is CRF01_AE [9-11]. Molecular epidemiological study of HIV resistance is likely to be problematic in areas where the dominant subtype is non-B, as various reports have shown that molecular markers related to drug resistance are less valid for non-B subtypes [12].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Antiretroviral Resistance Markers of Human Immunodeficiency Virus-1 of CRF01_AE Subtype in Bali, Indonesia

Budayanti

Merati

Bela

et al. 2019

CHR

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Background: Molecular epidemiological study of human immunodeficiency virus drug-resistant (HIVDR) markers is challenging in areas where the dominant subtype is non-B. Objective: Here we provide molecular data for HIVDR in the CRF01_AE subtype in Bali, Indonesia. Method: Seventy patients were enrolled in this study and grouped into treatment failure and treatment naïve groups. The full-length pol gene was amplified using nested reverse transcriptase polymerase chain reaction and the product was then sequenced. The readable sequence was then subjected to Stan-ford HIV Drug Resistance Database genotyping. Results: We found that clinical classification was in accordance with the presence of HIVDR markers in the pol gene. Independent of therapy history, the treatment failure group showed resistance markers against nucleoside reverse transcriptase inhibitors (NRTI) and non-nucleoside reverse transcriptase in-hibitors (NNRTI), ranging from 72%–100% of patients. Only a small proportion of naïve patients harbored HIV with drug resistance markers to NNRTI. No protease inhibitor-resistant marker was found in either patient group. Molecular marker mutations, which were found in more than 50% of treatment failure patients, were M184V (100%), T215A/Y/F (88.2%), D67N/G (76.5%), and M41L (58.8%). Conclusion: The protocol used in this study to determine genetic markers of HIVDR based on sub-type B can be applied for the rapid determination of resistance of the CRF01_AE subtype. All patients with progressive clinical signs and increased viral load should be recommended to undergo second-line treatment of the ARV regimen.

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Section: Discussioncontrasting

confidence: 91%

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Antiretroviral Resistance Markers of Human Immunodeficiency Virus-1 of CRF01_AE Subtype in Bali, Indonesia

Budayanti

Merati

Bela

et al. 2019

CHR

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“…Circulating recombinant form (CRF) 01_AE is one of the major CRFs of HIV-1 dominating the global epidemic and is prevalent throughout Southeast Asia. We previously reported that CRF01_AE was a dominant type of HIV-1 in Indonesia, while other subtypes and recombinants, including subtype B and the recombinant between CRF01_AE and subtype B, have emerged in Indonesia [2][3][4][5][6][7] .…”

Section: Textmentioning

confidence: 99%

Genetic Diversity and Drug Resistance of HIV-1 Circulating in North Sulawesi, Indonesia

Ueda

Witaningrum

Khairunisa

et al. 2019

AIDS Research and Human Retroviruses

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Manado, the capital city of North Sulawesi, is a unique region in Indonesia because of its religion. We collected peripheral blood samples from 63 individuals on antiretroviral therapy. The amplification of viral genomic fragments, viral subtyping, detection of HIV drug resistance-associated mutations (DRAMs), and phylogenetic analyses were performed. Viral subtyping revealed that the most prevalent HIV type 1 (HIV-1) subtype/circulating recombinant form (CRF) was CRF01_AE (84.1%), followed by subtype B (6.8%) and recombinants between CRF01_AE and CRF02_AG (4.5%). Although no major DRAMs were present in protease genes, they were detected in reverse transcriptase (RT) genes. Nine of 38 samples (23.7%) had major DRAMs against nucleoside RT inhibitors (NRTIs) and/or non-NRTIs. The results of phylogenetic analyses indicated that CRF01_AE in North Sulawesi is related to that in Bali. Therefore, Bali may play an important role in circulating CRF01_AE in North Sulawesi.

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Major Drug Resistance Mutations on Reverse Transcriptase Gene in Human Immunodeficiency Virus Type-1 in Indonesia: A Systematic Review

Nurjannah,

Jayanti,

Tanoerahardjo

et al. 2024

Curr HIV/AIDS Rep

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Appearance of Drug Resistance Mutations Among the Dominant HIV-1 Subtype, CRF01_AE in Maumere, Indonesia

Abstract: CRF01_AE was the major HIV-1 subtype prevalent in Maumere. The appearance of drug resistance-associated mutations found in the present study supports the necessity of monitoring the effectiveness of ART in Maumere.

Cited by 10 publications

References 41 publications

Molecular Antiretroviral Resistance Markers of Human Immunodeficiency Virus-1 of CRF01_AE Subtype in Bali, Indonesia

Molecular Antiretroviral Resistance Markers of Human Immunodeficiency Virus-1 of CRF01_AE Subtype in Bali, Indonesia

Genetic Diversity and Drug Resistance of HIV-1 Circulating in North Sulawesi, Indonesia

Major Drug Resistance Mutations on Reverse Transcriptase Gene in Human Immunodeficiency Virus Type-1 in Indonesia: A Systematic Review

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