Appearance and immunohistochemical localization of UDP-glucose: coniferyl alcohol glucosyltransferase in spruce (Picea abies (L.) Karst.) seedlings

Schmid, Günter; Hammer, D. K.; Ritterbusch, A.; Grisebach, Hans

doi:10.1007/bf00393726

Cited by 24 publications

(5 citation statements)

References 5 publications

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“…Even though most plant UGTs are predicted to occur in the cytoplasm, experimental evidence is varied. Cell fractionation studies have shown a number of UGTs to be localised to membranes (Loffelhardt and Kopp, 1981;Kalinowska and Wojciechowski, 1987;Warnecke and Heinz, 1994) although several other studies using cell fractionation, immunolocalisation or expression of GFP fusions have reported UGTs in the cytoplasm (Blume et al, 1979;Schmid et al, 1982;Yazaki et al, 1995;Dean et al, 2003;Achnine et al, 2005). The results from both our cell fractionation studies and GFP fusions strongly suggest that UGT85C2, UGT74G1 and UGT76G1 are located in the cytoplasm.…”

Section: Spatial Organisation Of the Steviol Glycoside Biosynthetic Psupporting

confidence: 52%

Spatial Organisation of Four Enzymes from Stevia rebaudiana that are Involved in Steviol Glycoside Synthesis

et al. 2006

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The sweet steviol glycosides found in the leaves of Stevia rebaudiana Bert. are derived from the diterpene steviol which is produced from a branch of the gibberellic acid (GA) biosynthetic pathway. An understanding of the spatial organisation of the two pathways including subcellular compartmentation provides important insight for the metabolic engineering of steviol glycosides as well as other secondary metabolites in plants. The final step of GA biosynthesis, before the branch point for steviol production, is the formation of (-)-kaurenoic acid from (-)-kaurene, catalysed by kaurene oxidase (KO). Downstream of this, the first committed step in steviol glycoside synthesis is the hydroxylation of kaurenoic acid to form steviol which is then sequentially glucosylated by a series of UDP-glucosyltransferases (UGTs) to produce the variety of steviol glycosides. The subcellular location of KO and three of the UGTs involved in steviol glycoside biosynthesis was investigated by expression of GFP fusions and cell fractionation which revealed KO to be associated with the endoplasmic reticulum and the UGTs in the cytoplasm. It has also been shown by expressing the Stevia UGTs in Arabidopsis that the pathway can be partially reconstituted by recruitment of a native Arabidopsis glucosyltransferase.

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Section: Spatial Organisation Of the Steviol Glycoside Biosynthetic Psupporting

confidence: 52%

Spatial Organisation of Four Enzymes from Stevia rebaudiana that are Involved in Steviol Glycoside Synthesis

et al. 2006

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“…Immunolocalisation has been employed in two instances. Schmid et al (1982) showed a cytosolic localisation of a coniferyl-GT in spruce seedlings, whilst Latchinian-Sadek and Ibrahim (1991) presented evidence of a vesicle-like and cytoplasmic membrane localisation of a¯avonol-B-ring speci®c GT in Chrysoplenium americanum. Ibrahim (1992) subsequently suggested that these results were indicative of a very loose association between the GT and the endoplasmic reticulum.…”

Section: Soup or Structure? ± The Organisation Of Glycosylationmentioning

confidence: 94%

“…Glycosyltransferases are also known to be developmentally regulated (Sparvoli et al 1994;Boss et al 1996;Miller et al 1999;Woo et al 1999;Kita et al 2000) and restricted in tissue localisation (Schmid et al 1982;Vogt and Taylor 1995;Boss et al 1996;Gong et al 1997;Ford and Hùj 1998;Martin et al 1999;Miller et al 1999). …”

Section: Soup or Structure? ± The Organisation Of Glycosylationmentioning

confidence: 98%

Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers

Jones

Vogt

2001

Planta

364

244

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Plants are exposed to a wide range of toxic and bioactive low-molecular-weight molecules from both exogenous and endogenous sources. Glycosylation is one of the primary sedative mechanisms that plants utilise in order to maintain metabolic homeostasis. Recently, a range of glycosyltransferases has been characterized in detail with regard to substrate specificity. The next step in increasing our understanding of the biology of glycosylation will require information regarding the exact role of individual glycosyltransferases in planta, as well as an insight into their potential involvement in metabolon-complexes. Hopefully, this will answer how a large number of glycosyltransferases with broad, rather than narrow, substrate specificity can be constrained in order to avoid interfering with other pathways of primary and secondary metabolism. These and other topics are discussed.

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“…Previous studies using cellular fractionation or immunolocalization have suggested cytosolic (Anhalt and Weissenböck, 1992; Blume et al. , 1979; Schmid et al. , 1982; Yazaki et al.…”

Section: Properties Of Triterpene Glycosyltransferasesmentioning

confidence: 99%

Genomics‐based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula

et al. 2005

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SummaryThe biosynthesis of triterpene saponins is poorly characterized in spite of the importance of these glycosylated secondary metabolites for plant defense and animal health. The model legume Medicago truncatula synthesizes more than 30 different saponins based on at least five triterpene aglycones; soyasapogenols B and E, medicagenic acid, hederagenin and bayogenin. We have employed an inducible cell culture system, DNA array-based and in silico transcript profiling, and targeted metabolite profiling, to identify triterpene glycosyltransferases (GTs) from among the more than 300 GTs expressed in M. truncatula. Two uridine diphosphate glucosyltransferases were functionally characterized; UGT73K1 with specificity for hederagenin and soyasapogenols B and E, and UGT71G1 with specificity for medicagenic acid. The latter enzyme also glycosylated certain isoflavones and the flavonol quercetin with higher efficiency than triterpenes; however, integrated transcript and metabolite profiling supported a function for UGT71G1 in terpenoid but not (iso)flavonoid biosynthesis in the elicited cell cultures.

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Appearance and immunohistochemical localization of UDP-glucose: coniferyl alcohol glucosyltransferase in spruce (Picea abies (L.) Karst.) seedlings

Cited by 24 publications

References 5 publications

Spatial Organisation of Four Enzymes from Stevia rebaudiana that are Involved in Steviol Glycoside Synthesis

Spatial Organisation of Four Enzymes from Stevia rebaudiana that are Involved in Steviol Glycoside Synthesis

Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers

Genomics‐based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula

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