Apoptotic effect of different self-etch dental adhesives on odontoblasts in cell cultures

El-Kholany, Naglaa Rezk; Abielhassan, Mohsen Hussein; ElEmbaby, Abeer; Maria, Ola M.

doi:10.1016/j.archoralbio.2011.11.019

Cited by 12 publications

(19 citation statements)

References 32 publications

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“…However, other studies have revealed that the main mechanism of cell death by bonding agents is apoptosis rather than necrosis (Mantellini et al ., ; El‐kholany et al ., ), which cannot be detected by the MTT assay. In early apoptosis, which is programmed cell death, metabolic collapse is not as obvious as it is in necrosis (Riss and Moravec, ) since the process of apoptosis requires energy (Ziegler and Groscurth, ).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

Lee

Park

et al. 2015

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Summary:The aim of this study was to evaluate the cytotoxic effects of one-step self-etching adhesives. Cells from an immortalized mouse odontoblast cell line (MDPC-23) were cultured with six different dental adhesive systems (diluted to concentrations of 0.5% for 4 h): Adper Easy Bond (EB), Xeno V (XV), iBond (IB), AdheSE One (AO), Clearfil SE primer (CS), and Adper Single Bond 2 (SB). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and flow cytometric apoptosis assays were used to evaluate cell viability and the rate of apoptosis. The odontoblasts were also examined under a scanning electron microscope. While all of the cultures with adhesives showed reduced viability, the viabilities in the IB and SB groups were not significantly different from the control group. Although increased apoptosis rates were observed in all of the cultures with adhesives, the rate in the SB group was not significantly different from the rate in the control. The control group showed the lowest apoptosis rate followed by the SB, AO, IB, EB, XV, and CS groups. When examined under a scanning electron microscope, control odontoblasts and the SB group exhibited relatively large cytoplasmic extensions. In contrast, in the EB and CS groups, fewer fibroblasts remained adhered to the plate surface. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, all cultures with one-step self-etching adhesives showed increased apoptotic activity. SB, an etch-and-rinse adhesive, was comparable to the control group, and CS and EB showed the lowest odontoblast viabilities according to the MTT assay. SCANNING 38:36-42, 2016.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

Lee

Park

et al. 2015

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“…1). Since satisfactory results were obtained with MTT assay and 1:10 dilution has been recommended 2) , this concentration was used in the following cytotoxicity test procedures.…”

Section: Discussionmentioning

confidence: 99%

“…Then the dentin bonding agent was light cured by LED (Elipar Freelight, 3M ESPE) for 20 s and the light source was kept 2 mm away from the base of vial during curing to simulate the clinical application. Medium (5 mL) was added per vial and incubated at 37 o C in 5% CO2 for 24 h. The incubated extract medium was filtered through a sterile 0.22 µm syringe filter 2) . The extract medium was added to the wells with three different concentrations [1:1 (1 mL extract medium), 1:10 (100 µL extract medium in 1 mL medium) and 1:20 (50 µL extract medium in 1 mL medium)] and then the cells were cultured with or without RES addition for 1 and 24 h respectively.…”

Section: Preparation Of Extracts and Test Specimensmentioning

confidence: 99%

“…One-component and onestep dentin bonding agents have been developed for easy application with the incorporation of the new monomers 2) . As the in vitro cytotoxicity of the resin monomers is reported in the studies [2][3][4][5][6][7] , there is concern about the biological safety of these materials. Dentin bonding agents may present different cytotoxicity by the interaction of the multiple components, thus the determination of the cytotoxicity of the product instead of the monomer is significant 3,8) .…”

Section: Introductionmentioning

confidence: 99%

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The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

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“…[25][26][27] Nagem-Filho et al 12 concluded that monomers become toxic only after their intimate contact with pulp or connective tissues. In additional, Hanks et al 28 suggested that low molecular weight monomers as HEMA show a greater cytotoxic effect than larger molecules due to their high interdiffusion ability through dentine tubules.…”

Section: Discussionmentioning

confidence: 99%

Effect on vascular permeability of a self-etching adhesive system containing an antimicrobial quaternary ammonium polymer QAMP into subcutaneous tissue of rats

Pupo

Farago

Nadal

et al. 2015

Archives of Oral Biology

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Apoptotic effect of different self-etch dental adhesives on odontoblasts in cell cultures

Cited by 12 publications

References 32 publications

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Effect on vascular permeability of a self-etching adhesive system containing an antimicrobial quaternary ammonium polymer QAMP into subcutaneous tissue of rats

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