Apoptosis-Inducing Activity of Marine Sponge<i>Haliclona</i>sp. Extracts Collected from Kosrae in Nonsmall Cell Lung Cancer A549 Cells

Bae, Wooli; Lim, Hyun; Kim, Kyoung Mee; Cho, Hyosun; Lee, Sun Yi; Jeong, Choon Sik; Lee, Hyi‐Seung; Jung, Joohee

doi:10.1155/2015/717959

Cited by 15 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colonies consisting of over 50 cells were counted by FluorChem E (San Jose, CA). Plating efficiency (PE) and survival fraction (SF) from the counted colonies were calculated according to Equations (2) and (3) , respectively 20 :…”

Section: Methodsmentioning

confidence: 99%

Chrysin Increases the Therapeutic Efficacy of Docetaxel and Mitigates Docetaxel-Induced Edema

et al. 2016

Self Cite

View full text Add to dashboard Cite

Docetaxel (DTX) is an effective commercial anticancer agent for chemotherapy in non–small cell lung cancer (NSCLC), breast cancer, gastric cancer, and prostate cancer, but its adverse effects including edema, neurotoxicity, and hair loss limit its application. To improve the chemotherapeutic efficacy of DTX and reduce adverse effects, combination therapy is one of the alternative methods. So chrysin, which has various biological activities including anticancer effects, was considered. In vitro, the combination of chrysin and DTX was investigated in A549 cells. Increased cytotoxicity, suppressed cellular proliferation, and induced apoptosis were observed with posttreatment of chrysin following DTX treatment. In vivo, chrysin enhanced the tumor growth delay of DTX and increased DTX-induced apoptosis in the A549-derived xenograft model. Furthermore, chrysin prevented DTX-induced edema in ICR mouse. These results indicated that chrysin strengthened the therapeutic efficacy of DTX and diminished the adverse effect of DTX, suggesting chrysin could be exploited as an adjuvant therapy for NSCLC.

show abstract

Section: Methodsmentioning

confidence: 99%

Chrysin Increases the Therapeutic Efficacy of Docetaxel and Mitigates Docetaxel-Induced Edema

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sponge extract of Haliclona sp. able to increase the percentage of apoptosis and significantly increase the expression of apoptotic gene p53, p21, caspase-8, and caspase-3 in A549 lung cancer cells 13 .…”

Section: Introductionmentioning

confidence: 92%

Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Tunjung

Sayekti

2019

F1000Res

View full text Add to dashboard Cite

Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane Ancorina sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50 of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry. Results: IC50 values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively. Conclusions: These results revealed that all extracts of Ancorina sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.

show abstract

“…This finding indicated that CA extract decreases the clonogenic property of individual cells of A549, synergistically as a result of cell proliferation assay. Compared to the previous A549 cells' clonogenic assays using a methanol extract of Haliclona sp., this result showed lower colony suppressing ability at a dose of 100 μg/mL, 23 but interestingly, the CA extract showed better performance for this assay at lower concentrations. It might be possible that the A549 cells are more sensitive toward CA extract compared to the extract of Haliclona sp.…”

Section: Discussionmentioning

confidence: 74%

Caspase Cascade Activation During Apoptotic Cell Death of Human Lung Carcinoma Cells A549 Induced by Marine Sponge Callyspongia aerizusa

Hadisaputri¹,

Andika²,

Sopyan³

et al. 2021

DDDT

View full text Add to dashboard Cite

In this study, Callyspongia aerizusa (CA), one of the most popular marine sponges for cancer therapy research, was investigated for its phytochemical compounds and evaluated for its anticancer activity in various cell lines. Since lung cancer is the most frequently diagnosed cancer, a solution from this marine source is a good choice to address the resistance to anticancer agents. Elucidation of the underlying mechanism of cell death elicited by a CA extract in human lung carcinoma cells A549 was undertaken. Methods: The presence of secondary metabolites in CA methanol extract was revealed by gas chromatography-mass spectrometry (GC-MS) and evaluated on four cancerous cell lines and a non-cancerous cell line using Cell Counting Kit-8. Since the activity of CA extract in A549 cells was then evaluated through clonogenic assay, morphological detection of apoptosis, polymerase chain reaction (PCR) and Western blot assay, were also presented in this study. Results: GC-MS analysis revealed the presence of two ergosteroids, ergost-22-en -3-one, (5β,22E), and ergost-7-en-3-ol, (35β) in the sponge extract that was suggested to suppress A549 cells (IC 50 9.38 μg/mL), and another cancerous cell's viability (IC 50 3.12-10.72 μg/mL) in 24 h, but not in the non-cancerous cells. Moreover, CA extract was also able to reduce the colony-forming ability of A549 cells, and through A549 cells morphology seems that apoptosis is the underlying mechanism of cell death. Further, the treatment with CA extract induced the up-regulation of caspase-9, caspase-3, and PARP-1, and the down-regulation of BCL-2, in both mRNA and proteins expression level, promoting apoptotic cell death via caspase cascade. Conclusion: These findings suggest that the compounds in CA extract possess the ability to induce apoptotic cell death in A549 cells and could become a promising candidate for future anticancer therapy.

show abstract

Apoptosis-Inducing Activity of Marine SpongeHaliclonasp. Extracts Collected from Kosrae in Nonsmall Cell Lung Cancer A549 Cells

Cited by 15 publications

References 23 publications

Chrysin Increases the Therapeutic Efficacy of Docetaxel and Mitigates Docetaxel-Induced Edema

Chrysin Increases the Therapeutic Efficacy of Docetaxel and Mitigates Docetaxel-Induced Edema

Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Caspase Cascade Activation During Apoptotic Cell Death of Human Lung Carcinoma Cells A549 Induced by Marine Sponge Callyspongia aerizusa

Contact Info

Product

Resources

About