Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Tunjung, Woro Anindito Sri; Sayekti, Puspa Restu

doi:10.12688/f1000research.17584.2

Cited by 7 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IC50 value less than 10 μg/mL was classified into powerful cytotoxicity effect. In contrast, strong cytotoxic effects have IC50 between 10 -100 μg/mL, moderate toxic effect category with IC50 values of 100 -500 μg/mL [54,57], and IC50 value more than 1000 μg/mL was categorized as no toxicity [55]. Our findings, in line with previous studies, indicate that EBRB does not exhibit cytotoxic effects on Vero cells.…”

Section: Cytotoxicity Assay Ebrb 'Sembada Hitam' On Vero Cellssupporting

confidence: 88%

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Aprilianti,

Purwestri,

Saragih

et al. 2024

J.Trop.Life.Science

View full text Add to dashboard Cite

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2-induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.

show abstract

Section: Cytotoxicity Assay Ebrb 'Sembada Hitam' On Vero Cellssupporting

confidence: 88%

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Aprilianti,

Purwestri,

Saragih

et al. 2024

J.Trop.Life.Science

View full text Add to dashboard Cite

show abstract

“…Another advantage of using this model is their ability to reproduce rapidly with high homogeneity, making them ideal for cytotoxicity testing [21]. Moreover, T47D cells can investigate apoptosis induction due to their capacity to express caspase-3 [22].…”

Section: Resultsmentioning

confidence: 99%

Effect of Black Rice Bran ‘Sembada Hitam’ on T47D Breast Cancer Cells

Cios Conara,

Oktavya,

Asih Purwestri

et al. 2023

JTLS

View full text Add to dashboard Cite

Indonesians have a rich tradition of incorporating black rice into their diet as a functional food due to its high antioxidant content. This research investigates the effects of the ethanolic extract of "Sembada Hitam" black rice bran on T47D breast cancer cells, specifically its cytotoxic, cell growth, and apoptotic induction properties. The MTT assay method was used to evaluate the cytotoxicity of the black rice bran extract on T47D cells after 24 and 48 hours of incubation. The acridine orange/propidium iodide (AO/PI) dye double staining method was employed to assess the apoptosis-inducing properties of the extract. Additionally, a cell growth assay was conducted to evaluate the effect of the extract on cell growth, with observations recorded on days 0, 3, and 6. The results revealed that the ethanolic extract of black rice bran "Sembada Hitam," when administered at various concentrations ranging from 7.81 to 1000 µg/mL, did not exhibit cytotoxic effects on T47D cells during the 24 and 48 hours of incubation. The cell growth assay revealed that T47D cells treated with the ethanolic extract of "Sembada Hitam" black rice bran at concentrations of 250 and 500 µg/mL exhibited lower growth rates than the 1% DMSO group from the 3rd to 6th day after incubation. The ethanolic extract of "Sembada Hitam" at concentrations of 250, 500, and 1000 µg/mL resulted in cell death of 10.64 ± 2.98%, 9.99 ± 5.87%, and 5.84 ± 0.78%, respectively. In conclusion, this study found that the ethanolic extract of "Sembada Hitam" black rice bran did not demonstrate significant cytotoxic effects on T47D breast cancer cells and is, therefore, unlikely to be a suitable candidate for an anti-cancer agent.

show abstract

“…Later, Tanjung and Sayekti in 2019 showed that Ancorina sp. extracts can induce apoptosis on T47D cell line [14]. Hasson et al managed to induce apoptosis in BT474 cell line using lyophilized camel's milk [15].…”

Section: Breast Cancer Cell Linesmentioning

confidence: 99%

Breast cancer research: in vitro models, markers, and in silico analysis - a narrative review

Šutković,

Lokvančić,

Sezer

2023

Bioengineering Studies

View full text Add to dashboard Cite

Breast cancer (BC) is one of the most common cancers among women worldwide but can also affect men. Although the detection and diagnosis of BC is advanced, there is a demand for more efficient approaches to treatment. This review summarizes the most important and latest discoveries in the diagnosis and prevention of breast cancer, using scientific discoveries done in vitro cell models, molecular and genetic markers, and data from different in silico analysis studies. Using Google Scholar and PubMed, scientific articles were searched systematically from inception to November 2023. As search terms in this review, we used: breast cancer, in vitro cell lines in BC, genetic and biochemical markers, and miRNA in BC. Based on the literature search, biochemical and genetic markers play essential roles in breast cancer research, whereas in vitro and silico research utilizes breast cancer cell models. Cell models are indispensable tools for the identification of potential new drugs, offering valuable findings on breast cancer growth dynamics. Molecular markers and innovative research methods not only aid in early detection but also contribute to our understanding of BC at various stages, offering treatment strategies and improving outcomes.

show abstract

Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Cited by 7 publications

References 39 publications

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation

Effect of Black Rice Bran ‘Sembada Hitam’ on T47D Breast Cancer Cells

Breast cancer research: in vitro models, markers, and in silico analysis - a narrative review

Contact Info

Product

Resources

About