APLIKASI METABOLOMIK BERBASIS HPLC UNTUK MENGIDENTIFIKASI WAKTU RETENSI KOMPONEN ANTIBAKTERI Stapylococcus aureus PADA EKSTRAK BUNGA KECOMBRANG (Etlingera elatior)

Maser, Wahyu Haryati; Yuliana, Nancy Dewi

doi:10.20961/alchemy.v13i2.4890

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…According to Ghasemzadeh et al [3], Kelantan torch ginger flower extract at a concentration of 10 mg/mL gave 8.40 mm inhibitory power, while Johor torch ginger flower extract gave 4.00 mm inhibitory power, and Pahang torch flower ginger extract gave 9.20 mm inhibitory power. A previous report showed that the chloroform extract had an inhibitory value of 3.78 mm at a concentration of 500 mg/mL [7]. Differences in torch ginger flower source, extraction method, and solvent used can affect the concentration of secondary metabolites in the plants, consequently, influencing their antibacterial activity [3,19].…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…According to some previous research, torch ginger blossom has an antibacterial effect against S. aureus [3,6]. Testing the antibacterial activity of S. aureus on local torch ginger flower has been carried out by using the modification well diffusion method [7]. However, the identification of components of the antibacterial compound of S. aureus from local torch ginger flower has not yet been conducted.…”

Section: ■ Introductionmentioning

confidence: 99%

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GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (Etlingera elatior) Flowers Extract

et al. 2022

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Torch ginger (Etlingera elatior) flowers are well known for their antibacterial effects against Staphylococcus aureus, however, the active compounds are still unknown. The purpose of this study was to conduct GC-MS-based metabolite profiling of torch ginger flower and identify compounds correlated with its S. aureus antibacterial activity using Orthogonal Projection to Latent Structure (OPLS). Using the well diffusion method, the antibacterial activity of ethanol extract, hexane, chloroform, and ethyl acetate fractions with a concentration of 80 mg/mL were investigated. The ethyl acetate fraction inhibited S. aureus growth the most (diameter of inhibition zone, DIZ 13.00–13.20 mm), while the hexane (DIZ 9.55–10.05 mm) and chloroform (DIZ 10.00–11.00 mm) fractions had moderate inhibitory activity, but the ethanol extract had no antibacterial effect. Using OPLS analysis, the GC-MS metabolite profile of all extracts and fractions was linked with the profile of antibacterial activity. This analysis revealed that Dodecanoic acid, 5-Tetradecene, and n-Hexadecanoic acid were identified as the compounds that were significantly connected with antibacterial activity.

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Section: Antibacterial Activitymentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (Etlingera elatior) Flowers Extract

et al. 2022

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“…Pada penelitian metabolomik, teknik pengujian menggunakan kromatografi cair kinerja tinggi (HPLC) yang dikombinasi dengan uji statistik multivariat digunakan untuk mengetahui profil metabolit seperti senyawa antibakteri dari ekstrak bunga kecombrang (Maser et al, 2017), komposisi senyawa fenol pada kulit citrus kering (Choi et al, 2011), komponen bioaktif daun torbangun (Yulianto et al, 2017), dan senyawa antioksidan dari rumput kebar (Aminudin et al, 2021). Demikian juga dengan penelitian-penelitian terhadap senyawa bioaktif jahe merah telah banyak diteliti, namun riset untuk mengetahui adanya korelasi antara aktivitas antioksidan dengan senyawa bioaktif dalam jahe merah menggunakan pendekatan metabolomik berbasis HPLC belum dilakukan.…”

Section: Alchemy Jurnal Penelitian Kimiaunclassified

Identifikasi Komponen Aktif Jahe Merah (Zingiber officinale Roscoe var. Rubrum) sebagai Sumber Antioksidan dengan Pendekatan Metabolomik Berbasis HPLC

et al. 2023

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Jahe merah (Zingiber officinale Roscoe var. Rubrum) merupakan tanaman rempah yang sering digunakan untuk pengobatan tradisional. Penelitian tentang senyawa bioaktif jahe merah telah banyak diteliti, namun penelitian untuk mengidentifikasi senyawa bioaktif sebagai sumber antioksidan menggunakan pendekatan metabolomik berbasis HPLC belum dilakukan. Tujuan dari penelitian ini adalah untuk mengidentifikasi komponen bioaktif jahe merah sebagai antioksidan dengan pendekatan metabolomik. Profil senyawa kimia dari ekstrak dan fraksi (heksana, kloroform, etil asetat, dan air) dianalisis menggunakan High Performance Liquid Chromatography (HPLC) yang dilanjutkan dengan analisis metabolomik Orthogonal Projection to Latent Structure (OPLS). Hasil uji metabolomik OPLS diperoleh fraksi etil asetat sebagai sampel yang paling berkontribusi terhadap aktivitas antioksidan. Sampel ini kemudian diidentifikasi menggunakan UHPLC-MS/MS dan diketahui bahwa komponen bioaktif atau specific marker pada jahe merah adalah senyawa [6]-gingerol. Kandungan senyawa [6]-gingerol merupakan senyawa yang paling dominan diikuti oleh 8-gingerol dan 10-gingerol dengan nilai masing-masing secara berurutan adalah 88,61±0,52; 23,59±0,41; dan 21,85±0,63 mg/g ekstrak.Identification of Active Components of Red Ginger (Zingiber officinale Roscoe var. Rubrum) as a Source of Antioxidants with HPLC-Based Metabolomics Approach. Red ginger (Zingiber officinale Roscoe var. Rubrum) is a rhizome used as a traditional medicine. Research on red ginger bioactive compounds has been widely studied but research to identify bioactive compound as source of antioxidant using HPLC-based metabolomic approaches has not been carried out. This study aims to identify the active compound of red ginger as an antioxidant with a metabolomic approach. Chemical profiles of the extracts and fractions (hexane, chloroform, ethyl acetate, and water) were analyzed using High Performance Liquid Chromatography (HPLC), followed by Orthogonal Projection to Latent Structure (OPLS) metabolomic analysis. The results of the OPLS metabolomic analysis showed that the ethyl acetate fraction is the sample that contributed the most to antioxidant activity. This sample was then identified using UHPLC-MS/MS showing that the bioactive component or specific marker in red ginger was [6]-gingerol. The [6]-gingerol content was the most dominant component, followed by [8]-gingerol and [10]-gingerol with values respectively 88.61±0.52, 23.59±0.41, and 21.85±0.63 mg/g extract.

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“…Table 2 shows that kecombrang has been used as a medicine for cough, wound, eye, and fever, and to improve breast milk production, remove odor, and fertilize hair. Several studies have shown that kecombrang's flowers had antibacterial activity (Maser et al 2017;Ghasemzadeh et al 2015;Wijekoon et al 2013;Abdelwahab et al 2010;Lachumy et al 2010). Kecombrang contains bioactive compounds such as polyphenols, alkaloids, flavonoids, steroids, saponins and essential oils which are thought to have potential as antioxidants and also alternative natural preservatives (Wijekoon et al 2011).…”

Section: The Patterns Of Kecombrang Cultivation In Agroforestry Systemsmentioning

confidence: 99%

The pattern of Etlingera elatior cultivation in agroforestry systems and its use as traditional medicines and food by local people of Kabanjahe, North Sumatra, Indonesia

2019

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Abstract. Purwoko A, Turnip H, Maser WH. 2019. The pattern of Etlingera elatior cultivation in agroforestry systems and its use as traditional medicines and food by local people of Kabanjahe, North Sumatra, Indonesia. Biodiversitas 20: 1998-2003. Etlingera elatior or kecombrang (Indonesian name) is a native plant of Indonesia. In Kabanjahe, this plant is planted in agroforestry systems. The purposes of this study were to describe the pattern of Etlingera elatior cultivation in agroforestry systems, and to document the use of Etlingera elatior as medicines and food by the local people of Kabanjahe, North Sumatra. Data were collected with field observations and interviews, and the collected data were analyzed qualitatively. The results showed that kecombrang was planted in agroforestry systems in three patterns, namely (i) kecombrang, cinnamon, and coffee, (ii) kecombrang, coffee, and bamboo, and (iii) and kecombrang, avocado, and vegetables. Kecombrang has been used as medicines for cough, wound, eye problems, fever, low breast milk production, odor, and hair thinning. In addition, kecombrang has also been used as a food ingredient in ubi tumbuk, ikan arsik, sambal kincung, getah tasak telu, cipera, terong dotak, gat-gat, and gulen manuk. With this research, it is suggested that agroforestry systems be developed to improve economic benefits for the local people of Kabanjahe.

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APLIKASI METABOLOMIK BERBASIS HPLC UNTUK MENGIDENTIFIKASI WAKTU RETENSI KOMPONEN ANTIBAKTERI Stapylococcus aureus PADA EKSTRAK BUNGA KECOMBRANG (Etlingera elatior)

Cited by 3 publications

References 8 publications

GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (<i>Etlingera elatior</i>) Flowers Extract

GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (<i>Etlingera elatior</i>) Flowers Extract

Identifikasi Komponen Aktif Jahe Merah (Zingiber officinale Roscoe var. Rubrum) sebagai Sumber Antioksidan dengan Pendekatan Metabolomik Berbasis HPLC

The pattern of Etlingera elatior cultivation in agroforestry systems and its use as traditional medicines and food by local people of Kabanjahe, North Sumatra, Indonesia

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