Apatite/Amelogenin Coating on Titanium Promotes Osteogenic Gene Expression

Du, Chang; Schneider, Galen B.; Zaharias, Rebecca; Abbott, Christopher; Seabold, Denise; Stanford, Clark M.; Moradian‐Oldak, Janet

doi:10.1177/154405910508401120

Cited by 46 publications

(52 citation statements)

References 23 publications

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“…In the present work, the cells were more rounded or cuboid, more regular and with shorter prolongations, as shown by other authors, [15,51]. However, authors described cells as having irregular edges and large numbers of filopodia or pseudopodia [16,52].…”

Section: Resultssupporting

confidence: 85%

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Behavior of Human Osteoblast Cells Cultured on Titanium Discs in Relation to Surface Roughness and Presence of Melatonin

Solá-Ruíz

Pérez-Martínez

Labaig-Rueda

et al. 2017

IJMS

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The aim of this work was to observe the behavior of osteoblast cells cultured in vitro on titanium discs in relation to disc surface roughness and the addition of melatonin to the culture medium. MG63 osteoblast cells were cultivated on 120 Grade 5 Ti divided into three groups: Group E, treated with dual acid etch; Group EP, treated with dual acid etch and calcium phosphate; and Group M, machined. Surface roughness was examined under a laser scanning confocal microscope (CLSM) and scanning electron microscopy (SEM). The proliferation and morphology of cells were determined under fluorescence microscopy and SEM. Messenger ribonucleic acid (mRNA) of different genes related to osteoblastic differentiation was quantified by means of real-time quantitative polymerase chain reaction (RT-PCR) assay. The greatest surface roughness was found in Group EP (Ra 0.354 µm), followed by Group E (Ra 0.266 µm), and Group M (Ra 0.131 µm), with statistically significant differences between the groups (p < 0.001). In the presence of melatonin a trend to a higher cell proliferation was observed in all groups although significant differences were only found in Group M (p = 0.0079). Among the genes studied, a significant increase in phosphate-regulating neutral endopeptidase, X-linked (PHEX) expression was observed in cells cultured on EP discs. The addition of melatonin increased osteoblast cell proliferation and differentiation, and may favor the osseointegration of dental implants.

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Section: Resultssupporting

confidence: 85%

“…Nevertheless, comparison of the present results with other studies is made difficult because of variations in cell culture times, genes and proteins quantified, quantification methods used, the surfaces on which cells were cultured, as well as the cell lines used [15,51,53]. …”

Section: Resultsmentioning

confidence: 96%

Behavior of Human Osteoblast Cells Cultured on Titanium Discs in Relation to Surface Roughness and Presence of Melatonin

Solá-Ruíz

Pérez-Martínez

Labaig-Rueda

et al. 2017

IJMS

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“…During the biomimetic approach, the osteoinductive protein is added to the modified simulated body fluid (m-SBF) solution and coprecipitated with the CaP coating onto the substrate material (Wen et al 1999;Du et al 2005;Liu et al 2007a). One disadvantage of this method is that the protein-incorporating rate reported is relatively low, with approximately 3 -15% of the proteins in the SBF being incorporated into the CaP coating (Liu et al 2004b(Liu et al , 2007bLuong et al 2006).…”

Section: Review Bone Tissue Engineering Y Liu Et Al S635mentioning

confidence: 99%

Biomimetic coatings for bone tissue engineering of critical-sized defects

Liu

Groot

2010

J. R. Soc. Interface.

124

119

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The repair of critical-sized bone defects is still challenging in the fields of implantology, maxillofacial surgery and orthopaedics. Current therapies such as autografts and allografts are associated with various limitations. Cytokine-based bone tissue engineering has been attracting increasing attention. Bone-inducing agents have been locally injected to stimulate the native bone-formation activity, but without much success. The reason is that these drugs must be delivered slowly and at a low concentration to be effective. This then mimics the natural method of cytokine release. For this purpose, a suitable vehicle was developed, the so-called biomimetic coating, which can be deposited on metal implants as well as on biomaterials. Materials that are currently used to fill bony defects cannot by themselves trigger bone formation. Therefore, biological functionalization of such materials by the biomimetic method resulted in a novel biomimetic coating onto different biomaterials. Bone morphogenetic protein 2 (BMP-2)-incorporated biomimetic coating can be a solution for a large bone defect repair in the fields of dental implantology, maxillofacial surgery and orthopaedics. Here, we review the performance of the biomimetic coating both in vitro and in vivo.

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“…In this way, the molecules can both sustain their biological activity for a considered period of time and support the mechanical properties of the coating in case of structural ECM components such as collagen. Both the biomimetic and electrospray deposition process (Table II) are among the most promising techniques for generating organic-inorganic composite coatings on implant materials due to their physiological process conditions (172)(173)(174).…”

Section: Organic-inorganic Composite Coatingsmentioning

confidence: 99%

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

et al. 2008

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Abstract. This paper reviews current physicochemical and biochemical coating techniques that are investigated to enhance bone regeneration at the interface of titanium implant materials. By applying coatings onto titanium surfaces that mimic the organic and inorganic components of living bone tissue, a physiological transition between the non-physiological titanium surface and surrounding bone tissue can be established. In this way, the coated titanium implants stimulate bone formation from the implant surface, thereby enhancing early and strong fixation of bone-substituting implants. As such, a continuous transition from bone tissue to implant surface is induced. This review presents an overview of various techniques that can be used to this end, and that are inspired by either inorganic (calcium phosphate) or organic (extracellular matrix components, growth factors, enzymes, etc.) components of natural bone tissue. The combination, however, of both organic and inorganic constituents is expected to result into truly bone-resembling coatings, and as such to a new generation of surface-modified titanium implants with improved functionality and biological efficacy.

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Apatite/Amelogenin Coating on Titanium Promotes Osteogenic Gene Expression

Cited by 46 publications

References 23 publications

Behavior of Human Osteoblast Cells Cultured on Titanium Discs in Relation to Surface Roughness and Presence of Melatonin

Behavior of Human Osteoblast Cells Cultured on Titanium Discs in Relation to Surface Roughness and Presence of Melatonin

Biomimetic coatings for bone tissue engineering of critical-sized defects

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

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