Antiviral, Cyototoxic And Antimicrobial Activities Of Anthraquinones Isolated From The Roots Of Morinda Elliptica

Ali, Abdul Manaf; Ismail, Nor Hadiani; Mackeen, Muhammad Mukram Mohamed; Yazan, Latifah Saiful; Mohamed, Shar Mariam; Ho, A.S.H.; Lajis, Nordin H.

doi:10.1076/1388-0209(200009)3841-aft298

Cited by 51 publications

(47 citation statements)

References 3 publications

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“…In the present result from the action of these terpenes. The only extract active against both strains (GM-MIC of 250 µg/mL) was that of M. royoc L. Extracts from other species such as M. elliptica and M. angustifolia displayed different biological activities, including antifungal (Xiang et al, 2008;Ali et al, 2000). Nevertheless, to our knowledge, the antifungal activity of M. royoc L has not been reported.…”

Section: Resultsmentioning

confidence: 71%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Atividade antifúngica in vitro e os efeitos citotóxicos de óleos essenciais e extratos de plantas medicinais e aromáticas contra Candida krusei e Aspergillus fumigatus" As plantas são geralmente utilizadas na medicina tradicional como agentes antimicrobianos e seus óleos essenciais e extratos foram conhecidos por possuir atividade antifúngica. O objetivo deste estudo foi avaliar in vitro a atividade de 32 óleos essenciais e 29 extratos contra Candida krusei e Aspergillus fumigatus, bem como o efeito citotóxico em células Vero. A curva do tempo-morte e a interação entre antifúngicos e Chenopodium ambrosioidese do extrato de Myrcia cucullata mostraram atividade antifúngica contra C. krusei (geometric means of the minimal inhibitory concentration [GM-MIC] 7,82 e 31,25 µg/ mL, respectivamente). Lippia citriodora foi ativa contra C. krusei e A. fumigatus (GM-CIM = 99,21 µg/mL e 62,5 µg/mL, respectivamente). Os testes de tempo-morte feitos com óleo de C. ambrosioides mostraram atividade fungicida em 4x MIC. A interação do óleo C. ambrosioides com itraconazol e anfotericina B foi testada pela técnica de xadrez. Nenhuma interação foi detectada pela combinação do óleo C. ambrosioides com anfotericina B e itraconazol (intervalo fractional inhibitory index [FICI] = 1,03-1,06 e 1,03-1,00, respectivamente). Os ensaios de citotoxicidade para todas as amostras foram realizadas com MTT. Apenas os óleos Hedyosmun sp. e L. dulcis foram citotóxicos.Unitermos: Candida krusei, Aspergillus fumigatus, óleos essenciais, extratos, curva do tempomorte, técnica de xadrez. ABSTRACT:The plants are usually used in traditional medicine as antimicrobial agents and their essential oils and extracts have been known to possess antifungal activity. The aim of this study was to evaluate the activity of 32 essential oils and 29 extracts in vitro against C. krusei and A. fumigatus as well as the cytotoxic effect on Vero cells. Time-kill curve and interaction between antifungal and the most active sample against C. krusei, was also evaluated. The oils from C. ambrosioides and the extract of M. cucullata showed antifungal activity against C. krusei (GM-MIC 7.82 and 31.25 µg/mL, respectively). L. citriodora was active against C. krusei and A. fumigates (GM-MIC = 99.21 µg/mL and 62.5 µg/mL respectively). Time-kill assays done with C. ambrosioides oil showed fungicidal activity at 4x MIC. The interaction of C. ambrosioides oil with itraconazole and amphotericin B was tested following the chequerboard technique. No interaction was detected for the combination of C. ambrosioides oil with amphotericin B and itraconazole (FICI range = 1.03-1.06 and 1.03-1.00, respectively). Cytotoxicity assays for all samples were carried out with MTT. Only the oil from Hedyosmun sp. and L. dulcis were cytotoxic.

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Section: Resultsmentioning

confidence: 71%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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“…Purified compounds with \10 lg/mL of cytotoxicity concentration are considered as promising cancer chemotherapy agents (Shier 1991). This cytotoxicity is attributed to the chemical structure of DAM and NDAM, as shown by Ali et al (2000) where the chemical structure between these compounds is tightly associated and the presence of the hydroxyl group at C-1 and C-3 and/or a formyl group at C-2 in the anthraquinones structure might be responsible for their cytotoxicity effects against several cancer cell lines. The selective cytotoxic effect of DAM and NDAM was supported by several previous studies.…”

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

et al. 2016

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Oral cancer is the eleventh most prevalent cancer worldwide. The most prevalent oral cancer is oral squamous cell carcinoma (OSCC). Damnacanthal (DAM) and nordamnacanthal (NDAM), the anthraquinone compounds, are isolated from the root of Morinda citrifolia L. (Noni), which has been used for the treatment of several chronic diseases including cancer. The objectives of this study were to evaluate the cytotoxicity, cell death mode, cell cycle, and the molecular mechanism of apoptosis induced by DAM and NDAM on OSCC. The cytotoxic effects of these compounds against OSCC cell lines were determined by MTT assay. The cell death mode was analysed by DNA laddering and FITC-annexin V/PI flow cytometric assays. In addition, the mechanism of apoptosis induced by DAM and NDAM was detected using mitochondrial membrane potential, Cytochrome c, and caspases assays. Finally, the effect of DAM and NDAM on cell cycle phase distribution of OSCC cells was detected by flow cytometry. In the present study, DAM and NDAM showed cytotoxicity towards OSCC cell lines and the maximum growth inhibition for both compounds was observed in H400 cells with IC 50 value of 1.9 and 6.8 lg/ml, respectively, after 72 h treatment. The results also demonstrated the inhibition of H400 OSCC cells proliferation, internucleosomal cleavage of DNA, activation of intrinsic apoptosis pathway, and cell cycle arrest caused by DAM and NDAM. Therefore, these findings suggest that DAM and NDAM can be potentially used as antitumor agents for oral cancer therapy.

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“…The increased number of G 1 population is not a consequence of perturbation on G 2 arrest because the total cell number decreased and more fragmented DNAs increased over time (data not shown). Interestingly, a previous screen by Ali et al identified Dam as an agent that protects against cytotoxicity caused by HIV [30]. Their screen was performed on CEM-SS cell infected with HIV and the mechanism of inhibition has remained unknown.…”

Section: Damnacanthal Inhibits Cell Death Without Affecting Vpr's G 2mentioning

confidence: 99%

Cell-based chemical genetic screen identifies damnacanthal as an inhibitor of HIV-1 Vpr induced cell death

Kamata

et al. 2006

Biochemical and Biophysical Research Communications

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Viral protein R (Vpr), one of the human immunodeficiency virus type 1 (HIV-1) accessory proteins, contributes to multiple cytopathic effects, G 2 cell cycle arrest and apoptosis. The mechanisms of Vpr have been intensely studied because it is believed that they underlie HIV-1 pathogenesis. We here report a cell-based small molecule screen on Vpr induced cell death in the context of HIV-1 infection. From the screen of 504 bioactive compounds, we identified Damnacanthal (Dam), a component of noni fruit, as an inhibitor of Vpr induced cell death. Our studies illustrate a novel efficient platform for drug discovery and development in anti-HIV therapy which should also be applicable to other viruses.

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Antiviral, Cyototoxic And Antimicrobial Activities Of Anthraquinones Isolated From The Roots Of Morinda Elliptica

Cited by 51 publications

References 3 publications

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia

Cell-based chemical genetic screen identifies damnacanthal as an inhibitor of HIV-1 Vpr induced cell death

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