Antiulcer Activity of the Most Active Sub-Fraction of Methanolic Leaf Extract of Buchanania Lanzan Spreng

Abstract

<p><strong>Objective: </strong>To evaluate the antiulcer<strong> </strong>activity of the most active sub-fraction of <em>Buchanania lanzan</em> Spreng. leaves methanolic extract (BLE).</p><p><strong>Methods: </strong>The antioxidant activity of BLE fractions and sub-fractions has been assayed to determine the most active sub-fraction by using <em>in vitro </em>antioxidant<em> </em>methods like hydrogen peroxide free radical scavenging assay, hydroxyl radical scavenging assay, DPPH (1, 1-diphenyl-2-picryl hydrazyl) radical scavenging activity, total flavonoid and total phenolic content estimation. Then, the antiulcerogenic activity of most active sub-fraction of BLE (50 and 100 mg/kg, b.w., orally) was evaluated employing<strong> a</strong>spirin+pylorus ligation-induced (APL) and HCl/ethanol-induced (HE) gastric ulcer models in rats, and histopathological examination of stomach tissues of rats.</p><p><strong>Results: </strong>The most active sub-fraction of BLE exerted a significant (<em>P<</em>0.01) dose-dependent decrease in the ulcerative lesion index produced by APL and HE ulcer models in rats as compared to the standard drugs omeprazole (30 mg/kg, b.w. orally) and ranitidine (32 mg/kg, b.w. orally) respectively. The reduction in gastric fluid volume, total acidity and an increase in the pH of the gastric fluid in APL treated rats proved the antisecretory activity of most active subfraction of BLE. From histopathological examination, it was found that in tissues of both the models that received pretreatment with most activesub-fraction showed better protection of the gastric mucosa in a dose-dependent manner as indicated by reduction or absence of mucosal erosion and infiltration of leucocytes.</p><p><strong>Conclusion: </strong>These results suggest that leaves of <em>Buchanania lanzan </em>Spreng.<em> </em>possess potential antiulcer activity, which may be attributed to its antioxidant mechanism of action.</p>

Evaluation of Antiulcerogenic Activity of Methanol Extracts of Brassica Oleracea Var. Capitata Rubra on Albino Rat Gastric Ulceration

“…The reduction in gastric fluid volume, total acidity, and an increase in the pH of the gastric fluid in APL treated rats proved the antisecretory activity of most active subfraction of BLE. The oral administration of most active subfraction (F4) of ethyl acetate fraction of methanolic leaf extract of B. lanzan Spreng produced significant antiulcer activity [31]. The antiulcer activity was investigated in M. pudica.…”

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“…The reduction in gastric fluid volume, total acidity, and an increase in the pH of the gastric fluid in APL treated rats proved the anti-secretory activity of most active sub-fraction of BLE. The oral administration of most active sub-fraction (F4) of ethyl acetate fraction of methanolic leaf extract of B. lanzan Spreng produced significant antiulcer activity 20 . Several scientific studies revealed that the phytoconstituents like flavonoids, tannins, terpenoids, and saponins were responsible for gastro protective agents 21,22 .…”

Evaluation of Antipyretic and Antiulcer Activity of Ethanolic Extract of Leaves of Alstonia Scholaris L. In Albino Wistar Rats

“…As a result, more and more drugs, both herbal and synthetic are coming up presenting newer and better options for the treatment of peptic ulcer [7]. Alstonia scholaris L., belongs to the family Apocynaceae as shown in Figs.…”