Antitumor Effects of Fucoidan Via Apoptotic and Autophagic Induction on HSC-3 Oral Squamous CellCarcinoma

Chantree, Pathanin; Surarak, Thanakorn; Sangpairoj, Kant; Aguilar, Panuroot; Hitakomate, Ekarat

doi:10.31557/apjcp.2020.21.8.2469

Cited by 10 publications

(8 citation statements)

References 43 publications

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“…These polysaccharides are used for various purposes especially in the food-processing industry, medicine, and biotechnology (Giavasis, 2014). Several previous studies suggested that fucoidan exerts the growth and proliferation inhibition in human cancer cells via apoptotic induction (Bai et al, 2020;Blaszczak et al, 2018;Chantree et al, 2020;Deepika et al, 2019;He et al, 2019;Liu et al, 2020;Narayani et al, 2019;Palanisamy et al, 2018). Moreover, some studies reported the effect of fucoidan in suppressing the invasion (Lin et al, 2017) and metastasis (Alekseyenko et al, 2007) of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Fucoidan via Apoptosis and Cell Cycle Arrest on Cholangiocarcinoma Cell

Chantree

Na‐Bangchang

Martviset

2021

Asian Pac J Cancer Prev

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Objective: Many previous studies reported that fucoidan has antitumor activities. The objective of the present study was to determine the cytotoxic effects and related mechanisms of cell death induced by fucoidan extracted from Fucus vesiculosus on CL-6 cholangiocarcinoma cell. Methods: CL-6 and OUMS cells were treated with 0, 100, 200, and 300 μg/mL of fucoidan. MTT assay was used to determine cytotoxicity. Flow cytometry-based assay was used to examine the distribution of apoptosis and cell cycle. The changes in nuclear morphology were determined using Hoechst 33,342 staining. Mitochondrial membrane potential (ΔΨm) was evaluated using the JC-1 kit. The apoptotic, anti-apoptotic, and cell cycle-related proteins study were examined by Western blot analysis. Results: The relative viable cell number of treated CL-6 cells was decreased but no effect was observed in OUMS normal cells. Furthermore, treated cells were arrested in the G0/G1 phase with down-regulation of cyclin D1 and CDK4. Annexin V/PI staining with flow cytometry analysis suggested that fucoidan could induce apoptosis in CL-6 cells. Western blot study revealed the up-regulation of apoptotic markers including Bax, cleaved PARP, cleaved caspase-3, but down-regulation of anti-apoptotic markers, cl-2. Moreover, fucoidan could induce nuclear fragmentation and chromatin condensation with alteration of ΔΨm. Conclusion: Fucoidan exerts antitumor properties against CL-6 cholangiocarcinoma cells illustrated by the induction of apoptosis and cell cycle arrest.

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Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Fucoidan via Apoptosis and Cell Cycle Arrest on Cholangiocarcinoma Cell

Chantree

Na‐Bangchang

Martviset

2021

Asian Pac J Cancer Prev

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“…; [4] Cerebral cortex or cerebellar lesions with a brainstem lesion diameter of > 1.5cm may be ischemic stroke caused by latent atherosclerosis of the great arteries. [5] Color ultrasound, transcranial Doppler ultrasound, or digital subtraction angiography can show the associated intracranial or extracranial artery stenosis or occlusion rate > 50%; In addition, the diagnosis of small artery stroke (symptomatic lacunar infarction) meets only one of its diagnostic criteria: typical clinical manifestations of lacunar infarction, and imaging findings showing stroke lesions <1.5cm are consistent with clinical symptoms.…”

Section: Methodsmentioning

confidence: 99%

“…With the rapid development of genetics, the genetics of stroke has gradually become a research hotspot in recent years. Studies have shown that genetic and environmental factors play an important role in the occurrence and development of stroke (4)(5)(6)(7)(8)(9). Genome-Wide Association Study (GWAS) based on single nucleotide polymorphisms (SNPs) is a powerful tool for studying complex diseases (10).…”

Section: Introductionmentioning

confidence: 99%

Common genetic variants on chromosome 9p21 confers risk of ischemic stroke: A large-scale genetic association study

Li¹,

Zhang²,

Yin³

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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The objective of this experiment was to observe the allele frequency and genotype distribution of some 9p21 SNPs in the Anhui Han population, and to study its relationship with the susceptibility to ischemic stroke. For this purpose, a collection of 992 patients with ischemic stroke confirmed and hospitalized in our hospital from October 2017 to October 2020 were used as the IS case group, and 951 normal people who had a healthy physical examination in the physical examination center of our hospital during the same period were selected as the control group. After informed consent, cubital venous blood of all subjects was collected, and epidemiological data of the subjects were collected; the rs2383206, rs2383207, rs10757274, and rs1333049 on chromosome 9p21 as the sites to be tested, using Sequenom Mass Array system for genotyping, using Haploview4.2 software to calculate whether the genotype distribution meets Hardy-Weinberg equilibrium. Results showed that there were no significant differences between the two groups in gender, age, and smoking history. There are significant differences in the levels of hypertension, diabetes and hyperlipidemia, total cholesterol, triglycerides, HDL-C and apolipoprotein A1 between the two groups of study subjects. The genotype frequencies of the participating populations were in a balanced state. The results of the association analysis between SNPs and IS susceptibility showed that rs2383207, rs10757274, rs1333049 and rs2383206 are the susceptibility sites of ischemic stroke. It concluded that in Anhui, China, the inheritance of chromosome 9p21 region is associated with ischemic stroke.

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“…Fucoidans, natural compounds, have excellent and obvious biological activities, including antitumor (Chantree, Surarak, Sangpairoj, Aguilar, & Hitakomate, 2020; Park, Kim, Nam, Deuk Kim, & Hyun Choi, 2011), anti‐inflammatory (Li, Yang, et al, 2021), anticoagulant (Yao, Zaw, Anderson, Hinds, & Yim, 2020), antibacterial (Ashayerizadeh, Dastar, & Pourashouri, 2020), and antioxidant activities (El Rashed et al, 2021). Especially in the aspect of antitumor activity, many studies have reported the prominent effect of fucoidan, including in prostate cancers (Rui, Pan, Shao, & Xu, 2017), breast cancers (Zhang et al, 2016), liver cancers (Wu et al, 2020), colorectal cancers (Bai et al, 2020), and ovarian cancers (Liu, Yang, Peng, Li, & Zhu, 2020).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of fucoidan on breast cancer cells and potential enhancement of their sensitivity to chemotherapy by regulating autophagy

Zhang

Xue

Wang

et al. 2021

Phytotherapy Research

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Fucoidan is a marine‐origin sulfated polysaccharide that has gained attention for its anticancer activities. However, the inhibitory effect of fucoidan on breast cancers by regulating autophagy and its mechanism are not clear, and the chemotherapeutic sensitization of fucoidan is largely unknown. In the present study, the anticancer potential of fucoidan was revealed in MCF‐7 and MDA‐MB‐231 cells. Additionally, we also studied the chemotherapeutic sensitization of fucoidan by combining chemotherapeutic drugs doxorubicin (ADM) and cisplatin (DDP) with fucoidan on breast cancer cells. In the two kinds of human breast cancer cells, cell viability was determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay. Apoptosis was examined with flow cytometry. Transfection assay was used to examine autophagy flow. Western blot was used to examine the expressions of related proteins. Results suggested that fucoidan could induce autophagy and might enhance the sensitivity of breast cancer cells to chemotherapeutic drugs. Mechanistically, fucoidan induced autophagy in breast cancer cells by down‐regulating m‐TOR/p70S6K/TFEB pathway. In conclusion, our research revealed that fucoidan could induce autophagy of breast cancer cells by mediating m‐TOR/p70S6K/TFEB pathway, thus inhibiting tumor development. Furthermore, fucoidan might enhance the sensitivity of breast cancer cells to ADM and DDP, and this enhancement was related to autophagy.

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Antitumor Effects of Fucoidan Via Apoptotic and Autophagic Induction on HSC-3 Oral Squamous CellCarcinoma

Cited by 10 publications

References 43 publications

Anticancer Activity of Fucoidan via Apoptosis and Cell Cycle Arrest on Cholangiocarcinoma Cell

Anticancer Activity of Fucoidan via Apoptosis and Cell Cycle Arrest on Cholangiocarcinoma Cell

Common genetic variants on chromosome 9p21 confers risk of ischemic stroke: A large-scale genetic association study

Inhibition of fucoidan on breast cancer cells and potential enhancement of their sensitivity to chemotherapy by regulating autophagy

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